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hiscript ii reverse transcriptase  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript ii reverse transcriptase
    Hiscript Ii Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+reverse+transcriptase+kit/HiScript+II+Reverse+Transcriptase/custom%40r201%4040091174
    Average 97 stars, based on 1496 article reviews
    hiscript ii reverse transcriptase - by Bioz Stars, 2026-10
    97/100 stars

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    Reverse Transcription:

    Article Title: Isolation, identification, and pathogenicity of Novel Duck Reovirus strain from ducklings in China.
    Article Snippet: .. Viral RNA was then reverse transcribed into complementary DNA (cDNA) with the HiScript II Reverse Transcriptase Kit (Vazyme, Nanjing, China). ..

    Article Title: Transcriptional architecture of African swine fever virus
    Article Snippet: .. Total RNA (1 μg) was purified and reverse-transcribed into cDNA via HiScript II Reverse Transcriptase (Vazyme, China) with a specific mix of primers following the manufacturer’s instructions. .. Using 0.2-2 ug of total RNA from ASFV-infected PAMs cells, reverse transcription (HiScript II Reverse Transcriptase [Vazyme, China]) was performed with strand-specific primers—forward primers for negative-strand and reverse primers for positive-strand-specific reverse transcription.

    Article Title: Revitalizing bioinert PCL: A biomimetic 3D-Printed gradient-pore scaffold with CSMA hydrogel-mediated BMP-2/QK delivery for vascularized bone regeneration.
    Article Snippet: Critical-sized bone defects exceed bone’s regenerative capacity and require space-maintaining implants that support vascular and bone-tissue ingrowth.. Three-dimensional (3D)-printed polycaprolactone (PCL) scaffolds are widely investigated for bone repair owing to controllable geometry, interconnected macropores, and structural stability.. However, uniform-pore designs cannot recapitulate heterogeneous bone porosity, while hydrophobic PCL has limited bioactivity.

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    Article Title: Environmentally relevant phthalic acid exposure promotes pulmonary fibrogenesis through IL-1α/NF-κB pathway: Implications for microplastic-associated respiratory health risks.
    Article Snippet: Total RNA was isolated from cells using Trizol reagent (Invitrogen). .. Subsequently, 1 μg of RNA was reverse-transcribed into cDNA with HiScript Reverse Transcriptase (Vazyme) following the manufacturer's protocol. .. Quantitative real-time PCR (RT-qPCR) was performed in triplicate using a LightCycler 480 II system (Roche) with ChamQ Universal SYBR qPCR Master Mix (Vazyme) (Shan et al., 2021).

    Article Title: Integrating in vivo metabolism, pharmacokinetics, and dual-indicator scoring for PK-marker selection of Celosiae Semen saponins.
    Article Snippet: The total saponins from Celosiae Semen (TSCS) show hepatoprotective and lipid-lowering effects against metabolic dysfunction-associated steatotic liver disease (MASLD).. However, their in vivo metabolic fate and holistic pharmacokinetic (PK) features remain largely uncharacterized.. In this study, an integrated PK-marker screening strategy was established by combining in vivo metabolic profiling, area under the curve (AUC)weighted composite PK analysis, and a dual-indicator scoring model that weights both systemic exposure and in vitro lipid-lowering activity.

    Article Title: Precision base-editing of the cryptic 3' acceptor site to correct the RNA splicing defect of β 654 -thalassemia.
    Article Snippet: .. Total RNA was reverse transcribed using the HiScript II Reverse Transcriptase Kit (Vazyme). ..

    Article Title: Comparative evaluation of three protein nanoscaffolds for IHNV G ectodomain display in rainbow trout (Oncorhynchusmykiss).
    Article Snippet: Infectious hematopoietic necrosis virus (IHNV) causes severe losses in rainbow trout (Oncorhynchus mykiss) aquaculture.. Vaccination is a critical control strategy, and the surface glycoprotein (G) is the primary target for protective immunity.. However, recombinant G protein alone is poorly immunogenic, prompting the use of selfassembling nanoparticle scaffolds to display its ectodomain and enhance immunogenicity.

    Purification:

    Article Title: Transcriptional architecture of African swine fever virus
    Article Snippet: .. Total RNA (1 μg) was purified and reverse-transcribed into cDNA via HiScript II Reverse Transcriptase (Vazyme, China) with a specific mix of primers following the manufacturer’s instructions. .. Using 0.2-2 ug of total RNA from ASFV-infected PAMs cells, reverse transcription (HiScript II Reverse Transcriptase [Vazyme, China]) was performed with strand-specific primers—forward primers for negative-strand and reverse primers for positive-strand-specific reverse transcription.

    Isolation:

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    cDNA Synthesis:

    Article Title: α-KG conformationally stabilizes nuclear GLUD1-SNAIL complex to fuel trans-endothelial migration in metastasis.
    Article Snippet: Quantify tube formation using Fiji (ImageJ) with the Angiogenesis Analyzer2 plugin to automatically assess endothelial network parameters (e.g., tube length, branching points). .. Total RNA was isolated using TRIzol Reagent, followed by cDNA synthesis with the HiScript Reverse Transcriptase kit (Vazyme). .. Quantitative PCR was performed on a CFX96 Real-Time System (BioRad) using SYBR Green PCR Master Mix (Bio-Rad) under the following conditions: 95 °C for 15 sec (denaturation), 60 °C for 30 sec (annealing/ extension) for 40 cycles.

    RNA Extraction:

    Article Title: Integrating in vivo metabolism, pharmacokinetics, and dual-indicator scoring for PK-marker selection of Celosiae Semen saponins.
    Article Snippet: The total saponins from Celosiae Semen (TSCS) show hepatoprotective and lipid-lowering effects against metabolic dysfunction-associated steatotic liver disease (MASLD).. However, their in vivo metabolic fate and holistic pharmacokinetic (PK) features remain largely uncharacterized.. In this study, an integrated PK-marker screening strategy was established by combining in vivo metabolic profiling, area under the curve (AUC)weighted composite PK analysis, and a dual-indicator scoring model that weights both systemic exposure and in vitro lipid-lowering activity.



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