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BIONOVAS Biotechnology Co Ltd hiscript i reverse transcriptase
Hiscript I Reverse Transcriptase, supplied by BIONOVAS Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hiscript+ii+reverse+transcriptase/hiscript+i+reverse+transcriptase/pmc03230593-203-11-12
Average 90 stars, based on 1 article reviews
hiscript i reverse transcriptase - by Bioz Stars, 2026-09
90/100 stars

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Reverse Transcription:

Article Title: Effect of dietary supplementation with Moringa oleifera leaf extract and Lactobacillus acidophilus on growth performance, intestinal microbiota, immune response, and disease resistance in whiteleg shrimp (Penaeus vannamei).
Article Snippet: This study investigated the effect of the moringa (Moringa oleifera) leaf extract and Lactobacillus acidophilus individually or combined on growth performance, enzyme activity, intestinal and hepatopancreatic histology, intestinal microbiota, immune response, and resistance against Vibrio alginolyticus and Vibrio parahaemolyticus in whiteleg shrimp (Penaeus vannamei).. Six diets were formulated: three diets without L. acidophilus containining 0 (control, ME0), 2.5 (ME2.5), and 5.0 g/kg of moringa (ME5.0) and the same three diets containing L. acidophilus at 1 × 107 CFU/g of diet (ME0+P, ME2.5 + P, and ME5.0 + P, respectively).. Growth performance was measured after 60 days of the rearing period.

Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
Article Snippet: RNA from SAS, HSC3, HT29, HCT15, H1299, A549, MCF7, and MDA-MB231 cells was extracted using TRIzol, and the total amount of RNA was quantified using a Nanodrop spectrophotometer (Thermo Fisher Scientific). .. One microgram of RNA was used for reverse-transcription with the HiScript I Reverse Transcriptase (Bionovas Biotechnology) according to the manufacturer’s instructions. .. For RT-qPCR, the reaction mixtures (20 μL) contained 10 μL of SYBR Green (Thermo Fisher Scientific), 0.5-μmol/L forward and reverse primers, and 0.1 μL of cDNA.

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

Article Title: Dietary of Lactobacillus paracasei and Bifidobacterium longum improve nonspecific immune responses, growth performance, and resistance against Vibrio parahaemolyticus in Penaeus vannamei.
Article Snippet: This study investigated the effects of two probiotics, namely Lactobacillus paracasei and Bifidobacterium longum, as feed additives on growth performance, nonspecific immunity, immune-related gene expression, and disease resistance against Vibrio parahaemolyticus in Penaeus vannamei.. The experimental diets were prepared using L. paracasei and B. longum at concentrations of 105 and 107 CFU/g; these diets were referred to as P5, P7, B5, and B7.. After 8 weeks of the diets, regarding growth performance, the B7 group showed the highest weight gain rate (890.34 ± 103.65%), special growth rate (4.08 ± 0.19%), and feed conversion rate (1.52 ± 0.19%) compared with the other groups.

Article Title: Glucagon-like peptide-1 receptor regulates receptor of advanced glycation end products in high glucose-treated rat mesangial cells
Article Snippet: .. For reverse transcription, 1 μg of RNA was incubated with 200 U of HiScript I reverse transcriptase (Bionovas Biotechnology, Toronto, Canada) in a buffer containing a final concentration of 20 mmol/L Tris/HCl (pH 7.8), 100 mmol/L NaCl, 0.1 mmol/L Ethylenediaminetetraacetic acid, 1 mmol/L Dithiothreitol, 50% glycerol, 2.5 mol/l poly (dT)12–18 oligomer, and 0.5 mmol/L of each dNTP at a final volume of 20 μL. ..

other:

Article Title: Functional characterization of Malabar grouper (Epinephelus malabaricus) interferon regulatory factor 9 involved in antiviral response.
Article Snippet: IRF9 is a crucial component in the JAK-STAT pathway.. IRF9 interacts with STAT1 and STAT2 to form IFN-Istimulated gene factor 3 (ISGF3) in response to type I IFN stimulation, which promotes ISG transcription.. However, the mechanism by which IFN signaling regulates Malabar grouper (Epinephelus malabaricus) IRF9 is still elusive.

Article Title: Phage display-derived alpaca nanobodies as potential therapeutics for Naja atra snake envenomation.
Article Snippet: Naja atra, the Chinese cobra, is a major cause of snake envenomation in Asia, causing hundreds of thousands of clinical incidents annually.. The current treat ment, horse serum-derived antivenom, has unpredictable side effects and presents manufacturing challenges.. This study focused on developing new-generation snake venom antidotes by using microbial phage display technology to derive nanobodies from an alpaca immunized with attenuated N. atra venom.

RNA Extraction:

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

cDNA Synthesis:

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

Synthesized:

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

Article Title: Dietary of Lactobacillus paracasei and Bifidobacterium longum improve nonspecific immune responses, growth performance, and resistance against Vibrio parahaemolyticus in Penaeus vannamei.
Article Snippet: This study investigated the effects of two probiotics, namely Lactobacillus paracasei and Bifidobacterium longum, as feed additives on growth performance, nonspecific immunity, immune-related gene expression, and disease resistance against Vibrio parahaemolyticus in Penaeus vannamei.. The experimental diets were prepared using L. paracasei and B. longum at concentrations of 105 and 107 CFU/g; these diets were referred to as P5, P7, B5, and B7.. After 8 weeks of the diets, regarding growth performance, the B7 group showed the highest weight gain rate (890.34 ± 103.65%), special growth rate (4.08 ± 0.19%), and feed conversion rate (1.52 ± 0.19%) compared with the other groups.

Quantitative RT-PCR:

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

Polymerase Chain Reaction:

Article Title: Development of an oral vaccine delivery system for shrimp aquaculture using attenuated Listeria monocytogenes
Article Snippet: Shrimp aquaculture faces major challenges from viral diseases, such as the white spot syndrome virus (WSSV), leading to high mortality and economic losses.. Vaccination offers a potential solution, but practical oral delivery systems remain limited.. Therefore, this study aims to elucidate the feasibility of using heat-killed attenuated Listeria monocytogenes (HKALM) as a safer alternative for plasmid delivery in shrimp.

Incubation:

Article Title: Glucagon-like peptide-1 receptor regulates receptor of advanced glycation end products in high glucose-treated rat mesangial cells
Article Snippet: .. For reverse transcription, 1 μg of RNA was incubated with 200 U of HiScript I reverse transcriptase (Bionovas Biotechnology, Toronto, Canada) in a buffer containing a final concentration of 20 mmol/L Tris/HCl (pH 7.8), 100 mmol/L NaCl, 0.1 mmol/L Ethylenediaminetetraacetic acid, 1 mmol/L Dithiothreitol, 50% glycerol, 2.5 mol/l poly (dT)12–18 oligomer, and 0.5 mmol/L of each dNTP at a final volume of 20 μL. ..

Concentration Assay:

Article Title: Glucagon-like peptide-1 receptor regulates receptor of advanced glycation end products in high glucose-treated rat mesangial cells
Article Snippet: .. For reverse transcription, 1 μg of RNA was incubated with 200 U of HiScript I reverse transcriptase (Bionovas Biotechnology, Toronto, Canada) in a buffer containing a final concentration of 20 mmol/L Tris/HCl (pH 7.8), 100 mmol/L NaCl, 0.1 mmol/L Ethylenediaminetetraacetic acid, 1 mmol/L Dithiothreitol, 50% glycerol, 2.5 mol/l poly (dT)12–18 oligomer, and 0.5 mmol/L of each dNTP at a final volume of 20 μL. ..



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