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hindiii digested lambda phage dna  (New England Biolabs)


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    New England Biolabs hindiii digested lambda phage dna
    Hindiii Digested Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11036 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hindiii+digested+lambda+dna/HindIII/pmc13179615-117-16-20
    Average 99 stars, based on 11036 article reviews
    hindiii digested lambda phage dna - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CHCHD2 mitigates age-associated proteostatic collapse by maintaining homeostasis of the mitochondria-lysosome axis.
    Article Snippet: .. MTS-BirA (empty vector), MTS-BirA-CHCHD2WT, and MTS-BirA-CHCHD2T61I plasmids were cut with HindIII, the 5’ overhang was blunted with Hot Start (New England Biolabs), and linearized plasmids cut with XhoI. ..

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Article Title: A wheat-specific proline-rich protein regulates grain weight through a ubiquitin-mediated delay of endosperm cellularization.
    Article Snippet: ligated into a PstI-digested pUC19-based intermediate vector carrying the Actin1 promoter from rice (OsActin1, GenBank accession S44221, 1.3 kb upstream region) and the nopaline synthase (Nos) terminator from Agrobacterium tumefaciens using T4 DNA ligase (M0202S, NEB), generating the Actin1:TaPGW1-A1:Ter expression cassette .. The resulting Actin1::TaPGW1-A1::Ter expression cassette was then excised with HindIII and ligated into the HindIIIdigested pUCBS vector using T4 DNA ligase (M0202S, NEB), generating the final biolistic transformation vector (Figure S13). ..

    Generated:

    Article Title: Reactivation of a loss-of-function SlGLK2 allele by frame-restoring genome editing in tomato
    Article Snippet: .. The pDe-Cas9_NTPII was generated by inserting a synthetic NTPII cassette into the Hind III-digested pDe-Cas9 fragment using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA, USA). .. The tt Lb Cas12a fragment was purified by gel extraction using the FastGene Gel/PCR Extraction Kit (NIPPON Genetics, Tokyo, Japan) and inserted into the Asc I-digested pDe-CAS9_NTPII vector using Ligation Mix (Takara, Shiga, Japan).

    Amplification:

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Polymerase Chain Reaction:

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Clone Assay:

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Construct:

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Sequencing:

    Article Title: Translocation-induced enhancer rewiring reveals cryptic oncogenic circuits in multiple myeloma.
    Article Snippet: .. Enhancer regions amplified from LP- 1 genomic DNA (2× SuperNova PCR Mix, GenStar; primers in table S11) were cloned into the pGL4.23[luc2/minP] vector (Promega) via SacI/HindIII (NEB) restriction sites and T4 DNA ligase (NEB), with constructs verified by sequencing. .. LP- 1 cells (5 × 105) were co- electroporated with 0.5 μg of enhancer- pGL4.23 and 5 ng of pRL- TK (Renilla control) using the Lonza 4D Nucleofector SF Kit (DS- 150 program).

    Expressing:

    Article Title: A wheat-specific proline-rich protein regulates grain weight through a ubiquitin-mediated delay of endosperm cellularization.
    Article Snippet: ligated into a PstI-digested pUC19-based intermediate vector carrying the Actin1 promoter from rice (OsActin1, GenBank accession S44221, 1.3 kb upstream region) and the nopaline synthase (Nos) terminator from Agrobacterium tumefaciens using T4 DNA ligase (M0202S, NEB), generating the Actin1:TaPGW1-A1:Ter expression cassette .. The resulting Actin1::TaPGW1-A1::Ter expression cassette was then excised with HindIII and ligated into the HindIIIdigested pUCBS vector using T4 DNA ligase (M0202S, NEB), generating the final biolistic transformation vector (Figure S13). ..

    Transformation Assay:

    Article Title: A wheat-specific proline-rich protein regulates grain weight through a ubiquitin-mediated delay of endosperm cellularization.
    Article Snippet: ligated into a PstI-digested pUC19-based intermediate vector carrying the Actin1 promoter from rice (OsActin1, GenBank accession S44221, 1.3 kb upstream region) and the nopaline synthase (Nos) terminator from Agrobacterium tumefaciens using T4 DNA ligase (M0202S, NEB), generating the Actin1:TaPGW1-A1:Ter expression cassette .. The resulting Actin1::TaPGW1-A1::Ter expression cassette was then excised with HindIII and ligated into the HindIIIdigested pUCBS vector using T4 DNA ligase (M0202S, NEB), generating the final biolistic transformation vector (Figure S13). ..

    other:

    Article Title: Protocol for eVLP-mediated CRISPR-Cas9 and prime editing in pancreatic cancer cell lines.
    Article Snippet: PBS, pH 7.2 Gibco Cat# 20012019 FBS Gibco Cat# 16000044 Hank’s Balanced Salt Solution (HBSS) Gibco Cat# 14025100 Polyethylenimine Polysciences Cat# 23966-2 PEG-it Virus Precipitation Solution System Biosciences Cat# LV810A-1 GoTaq G2 DNA Polymerase Promega Cat# M7841 Agarose Invitrogen Cat# 16500500 Polybrene Sigma-Aldrich Cat# TR-1003-G TAE Buffer 503 Geneflow Cat# B9-0030 T4 ligase Promega Cat# ADM1801 dNTP Promega Cat# ADU1240 Glycerol Fisher Scientific Cat# 10579570 Carbenicillin Sigma-Aldrich Cat# C1613 Purple 100bp ladder New England Biolabs Cat# N3231S PEI MAX Transfection Reagent Kyfora Bio Cat# 24765 Tryptone Melford Cat# 91079–40–2 Yeast extract Melford Cat# 8013-01-2 Potassium Chloride KCl Fisher Scientific Cat# 10375810 Glucose Fisher Scientific Cat# 10141520 Agar Sigma-Aldrich Cat# A1296 Stationary phase for GC, phase Synperonic PE/F68 Supelco Cat# 81112 Opti-MEM Reduced Serum Medium Gibco Cat#31985062 BsaI-HFv2 New England Biolabs Cat# R3733S XmaI New England Biolabs Cat# R0180S HindIII New England Biolabs Cat#R0104S Esp3I (BsmBI) Thermo Fisher Scientific Cat# ER0451 Critical commercial assays Wizard SV Genomic DNA Purification System Promega Cat# A2360 Monarch Spin Plasmid Miniprep Kit New England Biolabs Cat# T1110S Pierce BCA Protein Assay Kits Thermo Scientific Cat#23225 Wizard SV Gel and PCR Clean-Up System Promega Cat# A9281 Deposited data Human NQO1 Ensembl ENSG00000181019 Human EPHA8 (HEK3) Ensembl ENSG00000070886 Experimental models: Cell lines SUIT-2 Creative Bioarray Cat# CSC-C6655J PANC-1 Creative Bioarray Cat#CSC-C7095J MIA-PaCa-2 Creative Bioarray Cat#CSC-C9492L HEK293T ATCC Cat# CRL-3216 Oligonucleotides HEK site3 guide antisenseF (29-mer): caccgacgccctctggaggaagcagttta IDT DNA N/A HEK site3 guide antisenseR (29-mer): ctcttaaactgcttcctccagagggcgtc IDT DNA N/A HEK site 3 PEG-RT_PBS antisense for Tevo (84-mer): gtgcgggcccagact gagcacggattacaaggatgacgacgataagtgatggcagaggaaaggaagccctgcttcctccagagg IDT DNA N/A HEK site 3 PEG-RT_PBS antisense rev Tevo (84-mer): cgcgcctctggag gaagcagggcttcctttcctctgccatcacttatcgtcgtcatccttgtaatccgtgctcagtctgggccc IDT DNA N/A HEK site3 guide senseF (29-mer): caccggcccagactgagcacgtgagttta IDT DNA N/A HEK site3 guide senseR (29-mer): ctcttaaactcacgtgctcagtctgggcc IDT DNA N/A HEK site 3 PEG-RT_PBS sense for Tevo (74-mer): gtgctggaggaagca gggcttcctttcctctgccatcacttatcgtcgtcatccttgtaatccgtgctcagtct IDT DNA N/A HEK site 3 PEG-RT_PBS anti rev Tevo (74-mer): cgcgagactgagcacgg attacaaggatgacgacgataagtgatggcagaggaaaggaagccctgcttcctcca IDT DNA N/A sgRNA guide 1, 5′- CAC CGA ATG CAG AAT GCC ACT CTG- 3′ IDT DNA N/A sgRNA guide 2, 5′- CAC CGC CCG AAT TCA AAT CCT GGA-3′ IDT DNA N/A F1 HEK3 5′- CGG ATT ACA AGG ATG ACG ATA AGT G- 3′ IDT DNA N/A (Continued on next page) STAR Protocols 7, 104904, December 18, 2026 7 MATERIALS AND EQUIPMENT eVLP plasmid DNA mix CRITICAL: Make fresh for each use.



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