lambda dna hindiii digest (New England Biolabs)

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New England Biolabs lambda dna hindiii digest
Lambda Dna Hindiii Digest, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hindiii+digested+lambda+dna/Lambda+DNA-Hind+III+Digest/pm41433061-337-13-17
Average 95 stars, based on 92 article reviews

lambda dna hindiii digest - by Bioz Stars, 2026-09

95/100 stars

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Molecular Weight:

Article Title: Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1
Article Snippet: .. For molecular weight marker, 12.5 μg HindIII-digested phage lambda DNA (NEB, N3012) was dephosphorylated in 1X rCutSmart buffer (NEB, B6004) with 10 U of Quick-CIP (NEB, M0525) in a final volume of 30 μl for 1 h at 37°C. .. The phosphatase was inactivated at 80°C for 2 min and the DNA was purified through a Microspin G-50 column (Cytiva, 27533002) and stored at -20°C.

Marker:

Article Title: Chromosome-scale genome assembly of the European common cuttlefish Sepia officinalis
Article Snippet: The purity of gDNA was assessed using A260/280 and A260/230 absorbance ratios measured by NanoDrop spectrophotometer (ThermoFisher Scientific). .. The integrity of the obtained gDNA was checked using 0,75% agarose gel electrophoresis by loading 100 ng of gDNA sample and using Hind III digested Lambda DNA (NEB#3012) as a marker. .. DNA sequencing libraries were prepared from 500 ng of high MW gDNA using Illumina DNA PCR-Free Tagmentation Library Prep Kit (20041794) and UD Indexes Set A (20026121).

Article Title: Anellovirus protein encoded by ORF2/3 functions as the viral replication initiation protein.
Article Snippet: Membranes were blocked, incubated with IRDye800CW Streptavidin (LI- COR #926- 32230), and imaged using the Bio- Rad ChemiDoc MP Imaging System. .. A mixed ladder (Φ+λ) composed of ΦX174 DNA- HaeIII Digest (NEB N3026S) and Lambda DNA HindIII Digest (NEB 3012S) was used as a marker to determine DNA band size. ..

Lambda DNA Preparation:

Agarose Gel Electrophoresis:

Molecular Cloning:

Article Title: Universal nucleic acid preservation in biological fluids with boron clusters
Article Snippet: Barnase (cat no. MBS1146186) and Barstar (cat no. MBS1053017) were purchased from MyBioSource. .. RNase inhibitor was purchased from Molecular Cloning Laboratories (cat no. RNIN200). λ DNA-HindIII Digest (cat no. N3012) and dsRNA Ladder (cat no. N0363S) were purchased from New England BioLabs. λ DNA was purchased from Takara Bio (cat no. 3010). ssRNA was purchased from Millipore (cat no. 55714). tRNA (cat no. 10109495001) and MS2 single-stranded RNA genome (cat no. 10165948001) were purchased from Roche. .. Proteinase K was purchased from New England Biolabs (cat no. P8107S).

other:

Article Title: Augmenting off-the-shelf enzyme activities by multiple folds with a supramolecular complex additive
Article Snippet: Reactions containing 1X T4 ligase buffer (New England Biolabs, cat no. B0202S), 0.5 286 μg digested lambda DNA (New England Biolabs, cat no. N3012L), CHAPSO and 6NβCD at the 287 indicated concentrations, and T4 ligase (New England Biolabs, cat no. M0202L), in a total volume 288 of 10 μl.

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