merip and transcriptome sequencing (CloudSeq Biotech Inc)

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CloudSeq Biotech Inc merip and transcriptome sequencing
Merip And Transcriptome Sequencing, supplied by CloudSeq Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+throughput+transcriptome+sequencing/transcriptome+high+throughput+sequencing/pmc08640804-176-2-7
Average 90 stars, based on 1 article reviews

merip and transcriptome sequencing - by Bioz Stars, 2026-09

90/100 stars

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High Throughput Screening Assay:

Article Title: Interaction between the expression of hsa_circRPRD1A and hsa_circHERPUD2 and classical coronary risk factors promotes the development of coronary artery disease
Article Snippet: .. CloudSeq Biotech (Shanghai, China) performed high throughput transcriptome sequencing. .. RNA libraries were constructed by NEBNext® rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) and TruSeq Stranded Total RNA Library Prep Kit(Illumina, USA).

Article Title: Interaction between the expression of hsa_circRPRD1A and hsa_circHERPUD2 and classical coronary risk factors promotes the development of coronary artery disease.
Article Snippet: .. Data Availability CloudSeq Biotech (Shanghai, China) performed high throughput transcriptome sequencing. .. RNA libraries were constructed by NEBNext® rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) and TruSeq Stranded Total RNA Library Prep Kit(Illumina, USA).

Sequencing:

Article Title: Interaction between the expression of hsa_circRPRD1A and hsa_circHERPUD2 and classical coronary risk factors promotes the development of coronary artery disease.
Article Snippet: .. Data Availability CloudSeq Biotech (Shanghai, China) performed high throughput transcriptome sequencing. .. RNA libraries were constructed by NEBNext® rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) and TruSeq Stranded Total RNA Library Prep Kit(Illumina, USA).

other:

Article Title: Screening and molecular mechanism research on bile microRNAs associated with chemotherapy efficacy in perihilar cholangiocarcinoma
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-b-actin Abcam Cat#ab6276; RRID: AB_2223210 Rabbit monoclonal anti-SPARC Abcam Cat#ab207743; RRID: AB_2924283 Bacterial and virus strains Lv-miR-532-3p Genechem N/A Lv-sponge-miR-532-3p Genechem N/A Biological samples Bile samples Peking university cancer hospital (China) N/A Chemicals, peptides, and recombinant proteins Oxaliplatin MedChemexpress Cat#HY-17371 5-fluorouracil MedChemexpress Cat#HY-90006 Critical commercial assays High-throughput sequencing of miRNAs and mRNAs CloudSeq Biotech Inc N/A Lipofectamine RNAiMAX Invitrogen Cat#13778030 Lipofectamine 3000 Invitrogen Cat#L3000001 MicroRNA Reverse Transcription Kit EZBioscience Cat#EZB-miRT3 PrimeScriptTM RT Reagent Kit Takara Bio USA Cat#RR037A bicinchoninic acid protein assay kit Servicebio Cat#G2026-200T ECL Plus Western Blotting Detection Reagents Solarbio Cat#PE0010 Counting Kit-8 (CCK-8) kit Solarbio Cat#CA1210 Trizol reagent Thermo Fisher Cat#15596018CN Deposited data miRNA-seq count data This paper https://data.mendeley.com/datasets/6rx2k6xhjg/2 mRNA-seq count data This paper https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE280797 Experimental models: Cell lines QBC939 cells Qingqi (Shanghai) Biotechnology development Co., LTD Cat#BFN60810642 FRH0201 cells Otwo Biotech Cat#HTX2093 293T cell line Cell Bank of the Chinese Academy of Sciences SCSP-502 Oligonucleotides See Table S1 for sequences of miRNA mimics, inhibitor, and siRNA N/A N/A See Table S2 for primer sequences in qPCR N/A N/A Recombinant DNA pcDNA3.1-secreted protein acidic and rich in cysteine (SPARC) Genechem N/A control vector plasmids Genechem N/A (Continued on next page) iScience 27, 111437, December 20, 2024 e1

Article Title: Identification of potentially functional circRNAs and prediction of the circRNA-miRNA-hub gene network in mice with primary blast lung injury.
Article Snippet: The transcriptome high-throughput sequencing service was provided by CloudSeq Biotech (Shanghai, China).

Next-Generation Sequencing:

Article Title: Sequential Ultrasound-Triggered and Hypoxia-Sensitive Nanoprodrug for Cascade Amplification of Sonochemotherapy.
Article Snippet: Hypoxia, the typical and conspicuous characteristic of most solid tumors, worsens the tumor invasiveness and metastasis.. Here, we engineered a sequential ultrasound (US)/ hypoxia-sensitive sonochemotherapeutic nanoprodrug by initially synthesizing the hypoxia-activated azo bond-containing camptothecin (CPT) prodrug (CPT2-Azo) and then immobilizing it into the mesopores of sonosensitizer-integrated metal organic frameworks (MOF NPs).. Upon entering the hypoxic tumor microenvironment (TME), the structure of CPT2-Azo immobilized MOFs (denoted as MCA) was ruptured and the loaded nontoxic CPT2-Azo prodrug was released from the MOF NPs.

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