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high-throughput rna-sequencing microarray screening  (Arraystar inc)

 
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    Arraystar inc high-throughput rna-sequencing microarray screening
    Validation of individual circRNAs detected in <t>microarray</t> profiling using RT-qPCR. WT mice were exposed to Acid (0.1 N HCl) or LPS (1µg) for 1 day. BALF EVs and cells were collected and circRNAs were evaluated using qPCR (normalized to β-actin or GAPDH). (A) To validate the specific circRNAs using RT-qPCR, we first designed the specific primers targeting the BSJ region to detect circular forms of RNAs, as illustrated here (left panel). The specific sequence of each specific circRNAs is listed on the right panel. (B) Validation of specific circRNAs using RT-qPCR in BALF EVs. We selected five circRNAs that were highly altered in the BALF EVs using microarray profiling. Next, we validated the expression and alteration of each circRNA using RT-qPCR in BALF EVs. *p<0.05. (C) To confirm that the specific primers used to detect circRNAs fail to detect the linear form host RNA. Using circ30884 as an example, we confirmed that using the primer listed above, we failed to detect its host linear RNA XDH. The figures shown here represent repeats from three independent experiments. *p<0.05. (D) AMs and neutrophils were separately isolated from LPS-exposed mouse lung as the above. Circular RNAs were then detected using RT-qPCR. *p<0.05, ns, not significant.
    High Throughput Rna Sequencing Microarray Screening, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+throughput+microarray+platform/pmc11024224-138-4-9?v=Arraystar+inc
    Average 90 stars, based on 1 article reviews
    high-throughput rna-sequencing microarray screening - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections"

    Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1354676

    Validation of individual circRNAs detected in microarray profiling using RT-qPCR. WT mice were exposed to Acid (0.1 N HCl) or LPS (1µg) for 1 day. BALF EVs and cells were collected and circRNAs were evaluated using qPCR (normalized to β-actin or GAPDH). (A) To validate the specific circRNAs using RT-qPCR, we first designed the specific primers targeting the BSJ region to detect circular forms of RNAs, as illustrated here (left panel). The specific sequence of each specific circRNAs is listed on the right panel. (B) Validation of specific circRNAs using RT-qPCR in BALF EVs. We selected five circRNAs that were highly altered in the BALF EVs using microarray profiling. Next, we validated the expression and alteration of each circRNA using RT-qPCR in BALF EVs. *p<0.05. (C) To confirm that the specific primers used to detect circRNAs fail to detect the linear form host RNA. Using circ30884 as an example, we confirmed that using the primer listed above, we failed to detect its host linear RNA XDH. The figures shown here represent repeats from three independent experiments. *p<0.05. (D) AMs and neutrophils were separately isolated from LPS-exposed mouse lung as the above. Circular RNAs were then detected using RT-qPCR. *p<0.05, ns, not significant.
    Figure Legend Snippet: Validation of individual circRNAs detected in microarray profiling using RT-qPCR. WT mice were exposed to Acid (0.1 N HCl) or LPS (1µg) for 1 day. BALF EVs and cells were collected and circRNAs were evaluated using qPCR (normalized to β-actin or GAPDH). (A) To validate the specific circRNAs using RT-qPCR, we first designed the specific primers targeting the BSJ region to detect circular forms of RNAs, as illustrated here (left panel). The specific sequence of each specific circRNAs is listed on the right panel. (B) Validation of specific circRNAs using RT-qPCR in BALF EVs. We selected five circRNAs that were highly altered in the BALF EVs using microarray profiling. Next, we validated the expression and alteration of each circRNA using RT-qPCR in BALF EVs. *p<0.05. (C) To confirm that the specific primers used to detect circRNAs fail to detect the linear form host RNA. Using circ30884 as an example, we confirmed that using the primer listed above, we failed to detect its host linear RNA XDH. The figures shown here represent repeats from three independent experiments. *p<0.05. (D) AMs and neutrophils were separately isolated from LPS-exposed mouse lung as the above. Circular RNAs were then detected using RT-qPCR. *p<0.05, ns, not significant.

    Techniques Used: Biomarker Discovery, Microarray, Quantitative RT-PCR, Sequencing, Expressing, Isolation



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    Image Search Results


    Number of present miRNAs at different stages (day 0, day 12, day 19 and day 26) during cardiomyocyte specific differentiation and maturation.

    Journal: PLoS ONE

    Article Title: MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms

    doi: 10.1371/journal.pone.0025809

    Figure Lengend Snippet: Number of present miRNAs at different stages (day 0, day 12, day 19 and day 26) during cardiomyocyte specific differentiation and maturation.

    Article Snippet: In the present manuscript paper, a transgenic mouse ES cell clone was used to generate uniform Cor.At® cardiomyocytes. miRNAs were profiled for undifferentiated transgenic ES cells (day 0) and at time points day 12, day 19 and day 26 during cardiomyocyte-specific differentiation and maturation using 2 high throughput microarray platforms provided from Affymetrix and Febit.

    Techniques:

    The number of up/down regulated miRNAs at different maturation stages (day 12, day 19 and day 26) compared to undifferentiated ES cells were denoted with arrows up/down.

    Journal: PLoS ONE

    Article Title: MicroRNA Profiling during Cardiomyocyte-Specific Differentiation of Murine Embryonic Stem Cells Based on Two Different miRNA Array Platforms

    doi: 10.1371/journal.pone.0025809

    Figure Lengend Snippet: The number of up/down regulated miRNAs at different maturation stages (day 12, day 19 and day 26) compared to undifferentiated ES cells were denoted with arrows up/down.

    Article Snippet: In the present manuscript paper, a transgenic mouse ES cell clone was used to generate uniform Cor.At® cardiomyocytes. miRNAs were profiled for undifferentiated transgenic ES cells (day 0) and at time points day 12, day 19 and day 26 during cardiomyocyte-specific differentiation and maturation using 2 high throughput microarray platforms provided from Affymetrix and Febit.

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