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streptavidin ap  (Agilent technologies)


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    Structured Review

    Agilent technologies streptavidin ap
    Streptavidin Ap, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 25380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+resolution+slide+seq+spatial+transcriptomics+map/Streptavidin/pm11920617-56-11-13
    Average 99 stars, based on 25380 article reviews
    streptavidin ap - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Capybara Oil‐Based Nanoemulsion Attenuates Obesity‐Induced Renal Injury Through Modulation of Inflammation, Oxidative Stress, and Fibrosis in Mice
    Article Snippet: .. Subsequently, sections were incubated with antibodies, and these reactions were amplified using a biotin‐streptavidin system (Dako, Santa Clara, California, USA). .. Immunoreactive products were visualized using diaminobenzidine (DAB) reagent (Dako) and counterstained with hematoxylin.

    Article Title: CX3CL1–CX3CR1 axis promotes restraint stress-induced thymic involution through macrophage-mediated thymocyte apoptosis
    Article Snippet: Subsequently, sections were incubated overnight at 4°C with the following primary antibodies (Abs): goat anti-mouse CX3CL1 polyclonal antibody (pAb) (1:200, sc-7227, SantaCruz, Dallas, TX) or rabbit anti-mouse CX3CR1 pAb (1:100, PAB16479 , Abnova, Taipei city, Taiwan). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-goat or goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Article Title: Multiple simulated spaceflight stressors impact cardiac fibrosis, calcium dynamics, immune function, cytokines and gene variants in rat, Rattus norvegicus
    Article Snippet: .. Biotinylated secondary antibodies were incubated for 30 minutes at room temperature (anti-mouse Jackson Immuno 715-066-151 and anti-rabbit Jackson Immuno 715-066-152) followed by a 15-minute incubation with streptavidin-HRP (DAKO P039701-2). .. Antibody visualization was achieved with the DAB+ application (DAKO DAB + K346811-2).

    Article Title: ATI-2341 TFA promotes acquisition of epithelial-like immunophenotype in bone marrow mesenchymal stem cells and alleviates intrauterine adhesion in a rat model
    Article Snippet: After three washes with PBS, biotinylated goat anti-rabbit/mouse secondary antibody (1:200, Cat. No. E0432, Dako) was applied and incubated for 30 min at room temperature. .. Following another PBS wash, streptavidin-horseradish peroxidase (1:200, Cat. No. K0679, Dako) was applied and incubated for 30 min at room temperature. .. Diaminobenzidine (DAB) chromogen solution (Cat. No. K3468, Dako) was applied for 3-5 min in the dark; color development was monitored microscopically and stopped by rinsing with running water.

    Article Title: Chronic Excessive Exercise Aggravates Apical Periodontitis in Mice.
    Article Snippet: The sections were incubated overnight at 4°C with primary antibodies against IL- 6 (bs- 0782R) and IL- 10 (bs- 0698R), both rabbit polyclonal antibodies (Bioss Antibodies, Woburn, MA, USA), diluted 1:200, or with a rabbit polyclonal antiTNF- α antibody (NB600- 587, Novus Biologicals, Littleton, CO, USA), diluted 1:100. .. The sections were then incubated with a biotinylated secondary antibody for 40 min, followed by incubation with the streptavidin–peroxidase complex from the EnVision FLEX/HRP detection system (K8000; Dako, Glostrup, Denmark). ..

    Article Title: Immunohistochemical cerebral cortex expression of aquaporin 4, aquaporin 5, aquaporin 9 Vasopressin and GFAP in saltwater drowning and freshwater drowning.
    Article Snippet: Samples were dewaxed, hydrated, and microwave antigen unmasking with Dako Target-1X at 95 °C. .. The staining process was performed as follows: incubation with a blocking solution (1% H2O2 in 10% methanol with aluminum); wash buffer; incubation with bovine serum albumin; incubation at room temperature with primary antibodies in Dako REAL; wash buffer; incubation with biotinylated antibodies and diluent; wash buffer; incubation with antibodies Streptavidin-horseradish peroxidase; wash buffer; DAB (3,3’-diaminobenzidine); washed in distilled water and staining with hemalaum. .. A general and overall evaluation of each slide was performed by optical microscopy (Nikon Eclipse 50i).

    Amplification:

    Article Title: Capybara Oil‐Based Nanoemulsion Attenuates Obesity‐Induced Renal Injury Through Modulation of Inflammation, Oxidative Stress, and Fibrosis in Mice
    Article Snippet: .. Subsequently, sections were incubated with antibodies, and these reactions were amplified using a biotin‐streptavidin system (Dako, Santa Clara, California, USA). .. Immunoreactive products were visualized using diaminobenzidine (DAB) reagent (Dako) and counterstained with hematoxylin.

    Article Title: CX3CL1–CX3CR1 axis promotes restraint stress-induced thymic involution through macrophage-mediated thymocyte apoptosis
    Article Snippet: Subsequently, sections were incubated overnight at 4°C with the following primary antibodies (Abs): goat anti-mouse CX3CL1 polyclonal antibody (pAb) (1:200, sc-7227, SantaCruz, Dallas, TX) or rabbit anti-mouse CX3CR1 pAb (1:100, PAB16479 , Abnova, Taipei city, Taiwan). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-goat or goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotin-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions. ..

    Labeling:

    Article Title: Pharmacological preconditioning with cilostazol reverses steatosis and protects the liver against ischemia-reperfusion injury in steatotic rats
    Article Snippet: .. Biotinylated goat anti-rat and goat anti-rabbit IgG antibodies were used as secondary antibodies in a streptavidin-biotin complex peroxidase system (1:200; Labeled Streptavidin-Biotin 2 System horseradish peroxidase; DakoCytomation, Glostrup, Denmark). .. 3,3′-Diaminobenzidine (DakoCytomation, Glostrup, Denmark) served as chromogen.

    Staining:

    Article Title: Immunohistochemical cerebral cortex expression of aquaporin 4, aquaporin 5, aquaporin 9 Vasopressin and GFAP in saltwater drowning and freshwater drowning.
    Article Snippet: Samples were dewaxed, hydrated, and microwave antigen unmasking with Dako Target-1X at 95 °C. .. The staining process was performed as follows: incubation with a blocking solution (1% H2O2 in 10% methanol with aluminum); wash buffer; incubation with bovine serum albumin; incubation at room temperature with primary antibodies in Dako REAL; wash buffer; incubation with biotinylated antibodies and diluent; wash buffer; incubation with antibodies Streptavidin-horseradish peroxidase; wash buffer; DAB (3,3’-diaminobenzidine); washed in distilled water and staining with hemalaum. .. A general and overall evaluation of each slide was performed by optical microscopy (Nikon Eclipse 50i).

    Blocking Assay:

    Article Title: Immunohistochemical cerebral cortex expression of aquaporin 4, aquaporin 5, aquaporin 9 Vasopressin and GFAP in saltwater drowning and freshwater drowning.
    Article Snippet: Samples were dewaxed, hydrated, and microwave antigen unmasking with Dako Target-1X at 95 °C. .. The staining process was performed as follows: incubation with a blocking solution (1% H2O2 in 10% methanol with aluminum); wash buffer; incubation with bovine serum albumin; incubation at room temperature with primary antibodies in Dako REAL; wash buffer; incubation with biotinylated antibodies and diluent; wash buffer; incubation with antibodies Streptavidin-horseradish peroxidase; wash buffer; DAB (3,3’-diaminobenzidine); washed in distilled water and staining with hemalaum. .. A general and overall evaluation of each slide was performed by optical microscopy (Nikon Eclipse 50i).

    other:

    Article Title: Dual-function thermoresponsive antibiotic-loaded hydrogel with antimicrobial and osteogenic properties for implant-related infection control
    Article Snippet: Immunoreactivity was visualized with 3,3′-diaminobenzidine (DAB) as the chromogen (DAB Chromogen Kit, Dako Laboratories, CA, USA).



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    Spatial Transcriptomics Inc high resolution slide seq spatial transcriptomics map
    Fig. 1 | Single-cell and spatial <t>transcriptomics</t> of cardiac and ileum tissue of reovirus-infected neonatal mice. a, Experiment and analysis workflow. Four- day-old neonatal mice weighing 3 g per pup were infected (per os) with reovirus T1L. Neonatal mice infected with 1× PBS were used as mock controls. Ileum tissue (1 dpi and 4 dpi) and heart tissues (4 dpi, 7 dpi and 10 dpi) were assayed and used for scRNA-seq and spatial transcriptomics. b, UMAP plot of 31,684 single-cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi (one animal per condition), clustered by gene expression and colored by cell type (left). UMAP plots showing cardiac cell type clusters across samples for the heart scRNA-seq data (right). c, 8,243 spatial transcriptomes of cardiac tissue sections from mock-infected and reovirus-infected mice at 4 dpi and 7 dpi (one animal per condition). H&E-stained image of reovirus-infected myocarditic
    High Resolution Slide Seq Spatial Transcriptomics Map, supplied by Spatial Transcriptomics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+resolution+slide+seq+spatial+transcriptomics+map/seq+slide/pm36970396-158-20-22
    Average 86 stars, based on 1 article reviews
    high resolution slide seq spatial transcriptomics map - by Bioz Stars, 2026-10
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    Fig. 1 | Single-cell and spatial transcriptomics of cardiac and ileum tissue of reovirus-infected neonatal mice. a, Experiment and analysis workflow. Four- day-old neonatal mice weighing 3 g per pup were infected (per os) with reovirus T1L. Neonatal mice infected with 1× PBS were used as mock controls. Ileum tissue (1 dpi and 4 dpi) and heart tissues (4 dpi, 7 dpi and 10 dpi) were assayed and used for scRNA-seq and spatial transcriptomics. b, UMAP plot of 31,684 single-cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi (one animal per condition), clustered by gene expression and colored by cell type (left). UMAP plots showing cardiac cell type clusters across samples for the heart scRNA-seq data (right). c, 8,243 spatial transcriptomes of cardiac tissue sections from mock-infected and reovirus-infected mice at 4 dpi and 7 dpi (one animal per condition). H&E-stained image of reovirus-infected myocarditic

    Journal: Nature cardiovascular research

    Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

    doi: 10.1038/s44161-022-00138-1

    Figure Lengend Snippet: Fig. 1 | Single-cell and spatial transcriptomics of cardiac and ileum tissue of reovirus-infected neonatal mice. a, Experiment and analysis workflow. Four- day-old neonatal mice weighing 3 g per pup were infected (per os) with reovirus T1L. Neonatal mice infected with 1× PBS were used as mock controls. Ileum tissue (1 dpi and 4 dpi) and heart tissues (4 dpi, 7 dpi and 10 dpi) were assayed and used for scRNA-seq and spatial transcriptomics. b, UMAP plot of 31,684 single-cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi (one animal per condition), clustered by gene expression and colored by cell type (left). UMAP plots showing cardiac cell type clusters across samples for the heart scRNA-seq data (right). c, 8,243 spatial transcriptomes of cardiac tissue sections from mock-infected and reovirus-infected mice at 4 dpi and 7 dpi (one animal per condition). H&E-stained image of reovirus-infected myocarditic

    Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

    Techniques: Infection, Gene Expression, Staining

    Fig. 3 | Cytotoxic T cells recruited by inflamed endothelial cells induce pyroptosis in myocarditic tissue. a, UMAP plot of 9,786 single-cell endothelial cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by endothelial cell subtype clusters (phenotypes) (top) and condition (bottom). b, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for endothelial cell subtypes. c, UMAP plot showing the expression of genes upregulated in Cxcl9-high endothelial cells. d, Spatial transcriptomic maps of cardiac tissue from reovirus infected hearts at 7 dpi showing gene module scores calculated for four GO terms enriched in Cxcl9-high endothelial cells. e, UMAP plot of 2,205 single-cell T cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by T cell subtype clusters (top) and condition (bottom). f, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for T cell subtypes. g, UMAP plot showing the expression of genes upregulated in cytotoxic T cells from myocarditic heart at 7 dpi. h, Spatial transcriptomics maps of cardiac tissue from reovirus infected hearts at 7 dpi

    Journal: Nature cardiovascular research

    Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

    doi: 10.1038/s44161-022-00138-1

    Figure Lengend Snippet: Fig. 3 | Cytotoxic T cells recruited by inflamed endothelial cells induce pyroptosis in myocarditic tissue. a, UMAP plot of 9,786 single-cell endothelial cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by endothelial cell subtype clusters (phenotypes) (top) and condition (bottom). b, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for endothelial cell subtypes. c, UMAP plot showing the expression of genes upregulated in Cxcl9-high endothelial cells. d, Spatial transcriptomic maps of cardiac tissue from reovirus infected hearts at 7 dpi showing gene module scores calculated for four GO terms enriched in Cxcl9-high endothelial cells. e, UMAP plot of 2,205 single-cell T cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by T cell subtype clusters (top) and condition (bottom). f, Heat map showing top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for T cell subtypes. g, UMAP plot showing the expression of genes upregulated in cytotoxic T cells from myocarditic heart at 7 dpi. h, Spatial transcriptomics maps of cardiac tissue from reovirus infected hearts at 7 dpi

    Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

    Techniques: Infection, Expressing

    Fig. 4 | Myocarditic regions and the border zone have distinct transcriptomic profiles and cell-type-specific signatures. a, Spatial transcriptomics map of cardiac tissue section from reovirus-infected mice at 7 dpi colored by spot clusters representing transcriptionally distinct tissue regions. b, Spatial transcriptomics maps of cardiac tissue sections from reovirus-infected mice at 7 dpi showing the expression of differentially expressed genes of interest in the myocarditic and the border zone. c, Changes in average predicted cell type proportions across the infected ventricle, for cell types enriched in the myocarditic region and the border zone. d, UMAP plot of 502 single-cell cardiomyocyte cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by myocyte cell subtype (phenotypes) (left) and condition (right). e, Heat map showing the top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for cardiomyocyte cell subtypes. f, Venn diagram showing myocyte-specific genes

    Journal: Nature cardiovascular research

    Article Title: Spatiotemporal transcriptomics reveals pathogenesis of viral myocarditis.

    doi: 10.1038/s44161-022-00138-1

    Figure Lengend Snippet: Fig. 4 | Myocarditic regions and the border zone have distinct transcriptomic profiles and cell-type-specific signatures. a, Spatial transcriptomics map of cardiac tissue section from reovirus-infected mice at 7 dpi colored by spot clusters representing transcriptionally distinct tissue regions. b, Spatial transcriptomics maps of cardiac tissue sections from reovirus-infected mice at 7 dpi showing the expression of differentially expressed genes of interest in the myocarditic and the border zone. c, Changes in average predicted cell type proportions across the infected ventricle, for cell types enriched in the myocarditic region and the border zone. d, UMAP plot of 502 single-cell cardiomyocyte cell transcriptomes from mock-infected and reovirus-infected hearts at 4 dpi, 7 dpi and 10 dpi colored by myocyte cell subtype (phenotypes) (left) and condition (right). e, Heat map showing the top five differentially expressed genes (two-sided Wilcoxon test, log fold change > 1.0 and P < 0.01) for cardiomyocyte cell subtypes. f, Venn diagram showing myocyte-specific genes

    Article Snippet: UMAP plot showing the expression of myocyte-specific genes that are upregulated in the border zone of myocarditic regions (right). g, High-resolution Slide-seq spatial transcriptomics map of cardiac ventricular tissue from reovirus infected mice at 7 dpi colored by Slide-seq bead clusters.

    Techniques: Infection, Expressing