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96 well microplates  (Revvity)


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    Revvity 96 well microplates
    96 Well Microplates, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/SpectraPlate-96+HB%2C+Clear+96-well+Microplate+with+High+Protein+Binding+Capacity/ppr0631481-229-4-7
    Average 91 stars, based on 7 article reviews
    96 well microplates - by Bioz Stars, 2026-10
    91/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Protein Binding:

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Article Title: Tumor necrosis factor primes and metal particles activate the NLRP3 inflammasome in human primary macrophages
    Article Snippet: .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol. .. Enzyme-Linked immunosorbent assay (ELISA) The concentration of cytokines IL-1 β , TNF, IL-6, IL-1Ra, and IL-18 was measured from the culture supernatants using ELISA DuoSet kits (R&D) and high protein binding affinity 96-well microplates (SpectraPlate-96 HB, Perkin Elmer, Waltham, MA) following the manufacturers protocol.

    Incubation:

    Article Title: Optimal combination of beneficial mutations for improved ADCC effector function of aglycosylated antibodies.
    Article Snippet: The Fc region of IgG antibodies is crucial for binding to Fc receptors expressed on the surfaces of various immune leukocytes and eliciting therapeutic effector functions such as clearance of antibody-opsonized tumor cells.. Despite abrogated Fc gamma receptor (FcγR) binding and therapeutic effector function in the absence of Nlinked glycosylation at Asn297, the aglycosylated Fc region of IgG antibodies has bioprocessing advantages such as the absence of glycan heterogeneity and simple bacterial antibody production.. Therefore, these antibodies have been comprehensively engineered as effector functional units for human therapy.

    Article Title: Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice
    Article Snippet: .. Samples were serially-diluted and incubated on antigen-coated and blocked SpectraPlate-96 HB (PerkinElmer) plates. .. Samples were serially-diluted and incubated on antigen-coated and blocked SpectraPlate-96 HB (PerkinElmer) plates.

    Centrifugation:

    Article Title: An Fc variant with two mutations confers prolonged serum half-life and enhanced effector functions on IgG antibodies.
    Article Snippet: .. After centrifugation of the plate at 300 × g for 3min, 50 μl of supernatant from each well was transferred into a Spectraplate 96-well plate (PerkinElmer), and 50 μl of Cytotox 96® reagent (Promega) was added. ..



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    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Image Search Results


    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Journal: bioRxiv

    Article Title: Symmetric Dimethylarginine Modification Drives Shared and Divergent Antigen Reactivity in Lupus

    doi: 10.64898/2026.09.22.753525

    Figure Lengend Snippet: (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Article Snippet: High-binding ELISA plates (Greiner Bio-One, 655081) were coated with 250 ng/well of streptavidin (Leinco Technologies, S203) overnight at 4°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, Modification, Western Blot, Lysis, Clone Assay, Clinical Proteomics, Bioprocessing, Autoantibodies, Peptide Microarray, Produced

    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

    doi: 10.3389/fimmu.2026.1745166

    Figure Lengend Snippet: Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Article Snippet: For the MPO-specific ELISA kit, a 96-well high-binding polystyrene microplate (F-bottom, Greiner Bio-One, #655061) was used.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Control