Review



high binding polystyrene microplate  (Greiner Bio)


Bioz Verified Symbol Greiner Bio is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Greiner Bio high binding polystyrene microplate
    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
    High Binding Polystyrene Microplate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+F-Bottom+Clear/pmc13033559-99-7-11
    Average 97 stars, based on 875 article reviews
    high binding polystyrene microplate - by Bioz Stars, 2026-11
    97/100 stars

    Images

    1) Product Images from "Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study"

    Article Title: Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2026.1745166

    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
    Figure Legend Snippet: Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Infection, Control

    Related Articles

    Recombinant:

    Article Title: Recombinant Antigen for Newcastle Disease Virus Vaccine Focusing on Currently Circulating Virus Variants.
    Article Snippet: .. For anti-Castle1, anti-rotavirus recombinant protein, and anti-NDVBor-74 antibody titre determination, 96-well microplates (Greiner Bio-One 655001, Frickenhausen, Germany) were applied. ..

    96 Well Plates:

    Article Title: Structural optimization of donepezil-like hybrids toward an improved multi-target and ADME-related profile.
    Article Snippet: .. The assays were performed in clear flat-bottom, 96-well plates (Greiner BioOne GmbH, Fickenhausen, Germany). ..

    Article Title: Cross-reactive anti-prophage antibodies and bacterial heteroresistance implicated in phage therapeutic failure.
    Article Snippet: Phage therapy is an exciting strategy against antimicrobial-resistant bacterial infections, but critical knowledge gaps regarding its clinical application persist.. Here we present a case study of a 22-year-old male patient with cystic fibrosis, presenting with a recurrent, invasive and ultimately lethal Bordetella bronchialis infection, who failed compassionate-use phage therapy.. Using longitudinal clinical samples, we found that our patient harbored pre-existing antibodies against active prophages induced from the genome of the infecting pathogen.

    Microscopy:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Incubator:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Control:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Polymerase Chain Reaction:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Adhesive:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Incubation:

    Article Title: Turning RuBisCO-enriched snap pea protein concentrates into function: A novel plant protein for fat-free whipped cream alternatives
    Article Snippet: With the growing concerns over the environmental and ethical impacts associated with the use of animal-based protein, there is a need to explore new sustainable alternative protein sources.. In this study, the potential of a novel snap pea protein (SPP) as a functional ingredient for aerated food products development was evaluated.. Through the in-house established and validated miniaturized assay, the techno-functional properties, including emulsifying, foaming, and gelling properties of SPP were evaluated and compared with egg white protein (EWP) and mung bean protein (MBP).

    Saline:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Staining:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Transfection:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Modification:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Cell Culture:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Cell Counting:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Automated:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Sterility:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Qubit:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..



    Similar Products

    97
    Greiner Bio high binding elisa plates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+F-Bottom/bio_rxiv__64898__2026__09__22__753525-203-0-3
    Average 97 stars, based on 1 article reviews
    high binding elisa plates - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    95
    R&D Systems high binding microplates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding Microplates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Clear+Polystyrene+Microplates/pmc13058453-104-0-3
    Average 95 stars, based on 1 article reviews
    high binding microplates - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    97
    Greiner Bio high binding clear bottom plates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding Clear Bottom Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+384+Well+Ps+F-Bottom/pm41922952-83-2-6
    Average 97 stars, based on 1 article reviews
    high binding clear bottom plates - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    95
    Greiner Bio high binding
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+Half+Area+Black/us12590142-958-27-29
    Average 95 stars, based on 1 article reviews
    high binding - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    97
    Greiner Bio high binding 96 well plates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding 96 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+F-Bottom+Clear/pm41872358-228-0-3
    Average 97 stars, based on 1 article reviews
    high binding 96 well plates - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Greiner Bio high binding plates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+F-Bottom/pm41861097-53-3-6
    Average 97 stars, based on 1 article reviews
    high binding plates - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    95
    R&D Systems high binding 96 well plates
    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
    High Binding 96 Well Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Clear+Polystyrene+Microplates/pm41841171-85-4-7
    Average 95 stars, based on 1 article reviews
    high binding 96 well plates - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    97
    Greiner Bio high binding polystyrene microplate
    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
    High Binding Polystyrene Microplate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+F-Bottom+Clear/pmc13033559-99-7-11
    Average 97 stars, based on 1 article reviews
    high binding polystyrene microplate - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    95
    Greiner Bio high binding half area microplates
    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
    High Binding Half Area Microplates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Microplate+96+Well+Ps+Half+Area+White/pm41742422-306-13-16
    Average 95 stars, based on 1 article reviews
    high binding half area microplates - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    95
    Greiner Bio high binding microplate
    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
    High Binding Microplate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Elisa+Microplate+96+Well+Ps+C-Bottom/bio_rxiv__64898__2026__03__04__709591-269-3-5
    Average 95 stars, based on 1 article reviews
    high binding microplate - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    Image Search Results


    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Journal: bioRxiv

    Article Title: Symmetric Dimethylarginine Modification Drives Shared and Divergent Antigen Reactivity in Lupus

    doi: 10.64898/2026.09.22.753525

    Figure Lengend Snippet: (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Article Snippet: High-binding ELISA plates (Greiner Bio-One, 655081) were coated with 250 ng/well of streptavidin (Leinco Technologies, S203) overnight at 4°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, Modification, Western Blot, Lysis, Clone Assay, Clinical Proteomics, Bioprocessing, Autoantibodies, Peptide Microarray, Produced

    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

    doi: 10.3389/fimmu.2026.1745166

    Figure Lengend Snippet: Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Article Snippet: For the MPO-specific ELISA kit, a 96-well high-binding polystyrene microplate (F-bottom, Greiner Bio-One, #655061) was used.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Control