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high binding 96 well plates  (R&D Systems)


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    R&D Systems high binding 96 well plates
    High Binding 96 Well Plates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+microplate/Clear+Polystyrene+Microplates/pm41841171-85-4-7
    Average 95 stars, based on 93 article reviews
    high binding 96 well plates - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: The Fatty Acid Transporter CD36 Mediates Uptake, Biodistribution, and Cardioprotection by Small Extracellular Vesicles From HEK293 Cells
    Article Snippet: .. Samples were loaded on high‐binding 96‐well plates (R&D Systems, UK, DY990) and incubated overnight at 4°C. .. Then, wells were washed with DELFIA Wash buffer (PerkinElmer, 1244‐114) and incubated with 1% BSA/PBS for 1 h at room temperature (RT).

    Article Title: Post-mortem evidence of pathogenic angiogenesis and abnormal vascular function in early Alzheimer’s disease
    Article Snippet: The CD31 level, a marker of endothelial content, was measured in 1% SDS homogenates by a commercially available duoset ELISA kit, according to the manufacturer’s instructions (DY806-05, R&D systems). .. High-binding microplates (DY990, R&D Systems) were coated with capture antibody (1:120) and incubated for 16 h overnight. ..

    Article Title: The Fatty Acid Transporter CD36 Mediates Uptake, Biodistribution, and Cardioprotection by Small Extracellular Vesicles From HEK293 Cells.
    Article Snippet: .. Samples were loaded on high-binding 96-well plates (R&D Systems, UK, DY990) and incubated overnight at 4◦C. .. Then, wells were washed with DELFIA Wash buffer (PerkinElmer, 1244-114) and incubated with 1% BSA/PBS for 1 h at room temperature (RT).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Adrenomedullin-RAMP2 enhances endothelial cell maturation synergically with shear stress
    Article Snippet: .. The levels of soluble intercellular adhesion molecule-1 (ICAM-1) in the culture medium were measured using a human ICAM-1 ELISA kit (#DY720) and a Clear Microplate (#DY990) from R&D Systems (MN, USA). ..

    Article Title: Adrenomedullin-RAMP2 Enhances Lung Endothelial Cell Homeostasis Under Shear Stress.
    Article Snippet: .. The levels of soluble intercellular adhesion molecule-1 (ICAM-1) in the culture medium were measured using a human ICAM-1 ELISA kit (#DY720) and a Clear Microplate (#DY990) from R&D Systems (Minneapolis, MN, USA). ..

    Article Title: Adrenomedullin-RAMP2 Enhances Lung Endothelial Cell Homeostasis Under Shear Stress
    Article Snippet: .. The levels of soluble intercellular adhesion molecule-1 (ICAM-1) in the culture medium were measured using a human ICAM-1 ELISA kit (#DY720) and a Clear Microplate (#DY990) from R&D Systems (Minneapolis, MN, USA). ..

    Phospho-proteomics:

    Article Title: Salts of 2-fluoro-n-methyl-4-[7-(quinolin-6-yl-methyl)-imidazo[1,2-b][1,2,4]triazin-2-yl]benzamide and processes related to preparing the same
    Article Snippet: Briefly, histidine-tagged c-Met catalytic domain fusion protein (Invitrogen, #PV3143) was used for the assay. .. IC50 measurements were based on the degree of phosphorylation of poly Glu-Tyr (Sigma-Aldrich, #P0275) that was coated (0.01 mg/per well) on 96-well microplates (R&D systems, #DY990). .. The reaction was carried out in a 50 μL solution containing 50 mM HEPES (pH 7.5), 10 mM MnCl2, 10 mM MgCl2, 0.5 mM DTT, 100 μM Na3VO4, 5 μM ATP (Cell Signaling Technology, #9804) and serial dilutions of the test compound.

    Binding Assay:

    Article Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
    Article Snippet: Aliquots (0.5 mL) of plasma and serum were then stored in microcentrifuge tubes (1.5 mL tube, Sarstedt) using sterile transfer pipettes (Sarstedt) and frozen at −80°C for subsequent biochemical analyses. .. Serum intestinal fatty acid binding protein concentration ([I‐FABP]; DY990, R&D Systems, USA; dilution 1:5; intra‐assay CV 3.6%), plasma claudin 3 concentration ([CLDN‐3]; NBP2‐75328, Novus Biologicals, Littleton, CO, USA; neat; intra‐assay CV 6.1%), plasma lipopolysaccharide binding protein concentration ([LBP]; DY870‐05, R&D Systems, Minneapolis, MN, USA, USA; dilution 1:800; intra‐assay CV 3.0%), plasma soluble cluster of differentiation 14 concentration ([sCD14]; DY870‐05, R&D Systems; dilution 1:800; intra‐assay CV 3.4%) and plasma interleukin 6 concentration ([IL‐6]; HS600C, R&D Systems; neat; intra‐assay CV 3.4%) were analysed in duplicate using commercially available enzyme‐linked immunosorbent assay kits and quantified using a plate reader (MRX, Dynex Technologies, Chantilly, VA, USA). .. Serum C‐reactive protein concentration ([CRP]) was analysed on a Cobas 701 analyser (Roche, Burgess Hill, UK) using the Tina‐quant C‐Reactive Protein IV kit with values <1 mg L −1 coded as 0 in analysis.

    Protein Concentration:

    Article Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
    Article Snippet: Aliquots (0.5 mL) of plasma and serum were then stored in microcentrifuge tubes (1.5 mL tube, Sarstedt) using sterile transfer pipettes (Sarstedt) and frozen at −80°C for subsequent biochemical analyses. .. Serum intestinal fatty acid binding protein concentration ([I‐FABP]; DY990, R&D Systems, USA; dilution 1:5; intra‐assay CV 3.6%), plasma claudin 3 concentration ([CLDN‐3]; NBP2‐75328, Novus Biologicals, Littleton, CO, USA; neat; intra‐assay CV 6.1%), plasma lipopolysaccharide binding protein concentration ([LBP]; DY870‐05, R&D Systems, Minneapolis, MN, USA, USA; dilution 1:800; intra‐assay CV 3.0%), plasma soluble cluster of differentiation 14 concentration ([sCD14]; DY870‐05, R&D Systems; dilution 1:800; intra‐assay CV 3.4%) and plasma interleukin 6 concentration ([IL‐6]; HS600C, R&D Systems; neat; intra‐assay CV 3.4%) were analysed in duplicate using commercially available enzyme‐linked immunosorbent assay kits and quantified using a plate reader (MRX, Dynex Technologies, Chantilly, VA, USA). .. Serum C‐reactive protein concentration ([CRP]) was analysed on a Cobas 701 analyser (Roche, Burgess Hill, UK) using the Tina‐quant C‐Reactive Protein IV kit with values <1 mg L −1 coded as 0 in analysis.

    Intra Assay:

    Article Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
    Article Snippet: Aliquots (0.5 mL) of plasma and serum were then stored in microcentrifuge tubes (1.5 mL tube, Sarstedt) using sterile transfer pipettes (Sarstedt) and frozen at −80°C for subsequent biochemical analyses. .. Serum intestinal fatty acid binding protein concentration ([I‐FABP]; DY990, R&D Systems, USA; dilution 1:5; intra‐assay CV 3.6%), plasma claudin 3 concentration ([CLDN‐3]; NBP2‐75328, Novus Biologicals, Littleton, CO, USA; neat; intra‐assay CV 6.1%), plasma lipopolysaccharide binding protein concentration ([LBP]; DY870‐05, R&D Systems, Minneapolis, MN, USA, USA; dilution 1:800; intra‐assay CV 3.0%), plasma soluble cluster of differentiation 14 concentration ([sCD14]; DY870‐05, R&D Systems; dilution 1:800; intra‐assay CV 3.4%) and plasma interleukin 6 concentration ([IL‐6]; HS600C, R&D Systems; neat; intra‐assay CV 3.4%) were analysed in duplicate using commercially available enzyme‐linked immunosorbent assay kits and quantified using a plate reader (MRX, Dynex Technologies, Chantilly, VA, USA). .. Serum C‐reactive protein concentration ([CRP]) was analysed on a Cobas 701 analyser (Roche, Burgess Hill, UK) using the Tina‐quant C‐Reactive Protein IV kit with values <1 mg L −1 coded as 0 in analysis.

    Clinical Proteomics:

    Article Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
    Article Snippet: Aliquots (0.5 mL) of plasma and serum were then stored in microcentrifuge tubes (1.5 mL tube, Sarstedt) using sterile transfer pipettes (Sarstedt) and frozen at −80°C for subsequent biochemical analyses. .. Serum intestinal fatty acid binding protein concentration ([I‐FABP]; DY990, R&D Systems, USA; dilution 1:5; intra‐assay CV 3.6%), plasma claudin 3 concentration ([CLDN‐3]; NBP2‐75328, Novus Biologicals, Littleton, CO, USA; neat; intra‐assay CV 6.1%), plasma lipopolysaccharide binding protein concentration ([LBP]; DY870‐05, R&D Systems, Minneapolis, MN, USA, USA; dilution 1:800; intra‐assay CV 3.0%), plasma soluble cluster of differentiation 14 concentration ([sCD14]; DY870‐05, R&D Systems; dilution 1:800; intra‐assay CV 3.4%) and plasma interleukin 6 concentration ([IL‐6]; HS600C, R&D Systems; neat; intra‐assay CV 3.4%) were analysed in duplicate using commercially available enzyme‐linked immunosorbent assay kits and quantified using a plate reader (MRX, Dynex Technologies, Chantilly, VA, USA). .. Serum C‐reactive protein concentration ([CRP]) was analysed on a Cobas 701 analyser (Roche, Burgess Hill, UK) using the Tina‐quant C‐Reactive Protein IV kit with values <1 mg L −1 coded as 0 in analysis.

    Concentration Assay:

    Article Title: Faecal microbiome, gastrointestinal integrity, inflammation and thermoregulation in recent exertional heat illness patients and matched controls
    Article Snippet: Aliquots (0.5 mL) of plasma and serum were then stored in microcentrifuge tubes (1.5 mL tube, Sarstedt) using sterile transfer pipettes (Sarstedt) and frozen at −80°C for subsequent biochemical analyses. .. Serum intestinal fatty acid binding protein concentration ([I‐FABP]; DY990, R&D Systems, USA; dilution 1:5; intra‐assay CV 3.6%), plasma claudin 3 concentration ([CLDN‐3]; NBP2‐75328, Novus Biologicals, Littleton, CO, USA; neat; intra‐assay CV 6.1%), plasma lipopolysaccharide binding protein concentration ([LBP]; DY870‐05, R&D Systems, Minneapolis, MN, USA, USA; dilution 1:800; intra‐assay CV 3.0%), plasma soluble cluster of differentiation 14 concentration ([sCD14]; DY870‐05, R&D Systems; dilution 1:800; intra‐assay CV 3.4%) and plasma interleukin 6 concentration ([IL‐6]; HS600C, R&D Systems; neat; intra‐assay CV 3.4%) were analysed in duplicate using commercially available enzyme‐linked immunosorbent assay kits and quantified using a plate reader (MRX, Dynex Technologies, Chantilly, VA, USA). .. Serum C‐reactive protein concentration ([CRP]) was analysed on a Cobas 701 analyser (Roche, Burgess Hill, UK) using the Tina‐quant C‐Reactive Protein IV kit with values <1 mg L −1 coded as 0 in analysis.



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    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by <t>ELISA</t> using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a <t>microplate</t> reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.
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    (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Journal: bioRxiv

    Article Title: Symmetric Dimethylarginine Modification Drives Shared and Divergent Antigen Reactivity in Lupus

    doi: 10.64898/2026.09.22.753525

    Figure Lengend Snippet: (A) Sera from MRL lpr mice (n=12, bled weekly from 8 to 13 weeks of age) were analyzed by ELISA using synthetic 10×RG peptides: native sequence (GRGRGRGRGRGRGRGRGRGR) vs. SDMA-modified (lowercase ‘r’ denotes SDMA; GrGrGrGRGRGrGrGrGRGR). All 12 mice developed anti SDMA antibody titers above the corresponding native controls, and the majority (9/12) showed a gradual increase in anti-SDMA titers over time. (B) Immunoblotting of HEp-2 lysates revealed progressive changes in band patterns from 10 weeks onward. To identify SDMA specific reactive proteins, cells were pretreated with the methyltransferase inhibitor EPZ015666 (EPZ) before lysis. A prominent ∼15 kDa band (arrowhead) was markedly reduced by EPZ pretreatment, supporting its recognition by anti SDMA antibodies. (C) Sera from three 12 week old MRL lpr littermates (Lpr 1, 2, 3) each showed distinct band patterns against the cell lysates. EPZ pretreatment of the cells reduced the intensity of several bands in all three mice. (D) Workflow for isolating hybridoma clones from individual plasma cells to obtain monoclonal antibodies (mAbs) specific for SDMA. MRL lpr mice were not immunized with any antigen; thus, the resulting mAbs represent naturally arising autoantibodies. (E) Two antibody clones were tested against the native versus SDMA peptide array shown in Figurè3. Clone P4A2F4 reacted extensively with most SDMA containing peptides, whereas clone P2C1C10 showed no reactivity. (F) The two mAbs produced different band patterns on cell lysates. P4A2F4 recognized bands that were reduced in EPZ pretreated lysates, consistent with SDMA specificity. (G) After implantation of the SDMA-reactive hybridoma clone into the peritoneal cavity of healthy BALB/c mice, the kidneys showed extensive IgG deposition, reminiscent of human lupus nephritis.

    Article Snippet: High-binding ELISA plates (Greiner Bio-One, 655081) were coated with 250 ng/well of streptavidin (Leinco Technologies, S203) overnight at 4°C.

    Techniques: Enzyme-linked Immunosorbent Assay, Sequencing, Modification, Western Blot, Lysis, Clone Assay, Clinical Proteomics, Bioprocessing, Autoantibodies, Peptide Microarray, Produced

    Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

    doi: 10.3389/fimmu.2026.1745166

    Figure Lengend Snippet: Detection of cathelicidin (LL-37) and myeloperoxidase (MPO) in human urine samples. Protein concentrations in human urine samples were detected by ELISA. The samples were analyzed in a microplate reader. Bar represents mean ± SD of each group. Protein levels were compared across groups (A, C) and subgroups (B, D). (A, C) LL-37 and MPO concentrations are higher in infected (Group A and B) than in healthy urines (Control). (B, D) No difference in protein concentrations was found when comparing the subgroups of Group A. ****p < 0.0001.

    Article Snippet: For the MPO-specific ELISA kit, a 96-well high-binding polystyrene microplate (F-bottom, Greiner Bio-One, #655061) was used.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Control