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fetal osteoblast cell line hfob1 19  (ATCC)


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    ATCC fetal osteoblast cell line hfob1 19
    Fetal Osteoblast Cell Line Hfob1 19, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfob1+19+cells/hFOB+1%2E19/pm41898628-131-13-19
    Average 98 stars, based on 840 article reviews
    fetal osteoblast cell line hfob1 19 - by Bioz Stars, 2026-10
    98/100 stars

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    Cell Culture:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..

    Article Title: Porphyromonas gingivalis lipopolysaccharide induces osteoblast senescence and defective mineralization associated with indoleamine 2,3-dioxygenase 1-kynurenine-aryl hydrocarbon receptor signaling.
    Article Snippet: Aim: To determine whether Porphyromonas gingivalis-derived lipopolysaccharide (P.g-LPS) is associated with osteoblast senescence and defective mineralization involving inflammation-related indoleamine 2,3-dioxygenase 1 (IDO1)–kynurenine (Kyn)–aryl hydrocarbon receptor (AhR) pathway and to examine its upstream regulation.. Methods: hFOB1.19 human osteoblasts and zebrafish were exposed to P.g-LPS.. Osteogenic differentiation, senescence, IDO1/Kyn/AhR signaling, Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB)/RELA regulation, and rescue by the IDO1 inhibitor epacadostat or AhR inhibitor CH223191 were assessed using staining, qPCR, western blotting, ELISA, gene silencing, and dual-luciferase reporter assays.

    Concentration Assay:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Incubator:

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..

    In Vitro:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..

    Activity Assay:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..

    Functional Assay:

    Article Title: Bioactive Multilayer Poly(ε-caprolactone)–Chitosan Coatings for Spinal Screws
    Article Snippet: .. The hFOB 1.19 human cell line (American Type Culture Collection, ATCC, Manassas, VA, USA) was employed as the in vitro model to investigate the biocompatibility of PCLbased coatings, as well as to assess their capacity to sustain osteoblastic activity and functional maturation. ..



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