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human osteoblast cell line hfob1 19  (ATCC)


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    ATCC human osteoblast cell line hfob1 19
    Human Osteoblast Cell Line Hfob1 19, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 836 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hfob1+19+cells/hFOB+1%2E19/pm41896887-56-16-24
    Average 98 stars, based on 836 article reviews
    human osteoblast cell line hfob1 19 - by Bioz Stars, 2026-10
    98/100 stars

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    Cell Culture:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..

    Article Title: Single-cell transcriptomics reveals glycolytic heterogeneity and identifies STC2 as a key regulator of metabolic reprogramming in osteosarcoma.
    Article Snippet: 1 Department of Orthopedic Surgery, Shengli Clinical Medical College, Fujian Medical University, Affiliated Provincial Hospital of Fuzhou University, No. 134 East Street, Fuzhou 350001, China Abstract Osteosarcoma (OS) exhibits profound metabolic reprogramming, yet the specific cellular subpopulations and regulatory networks governing glycolysis within the tumor microenvironment remain elusive.. We integrated single-cell RNA sequencing (scRNA-seq) data from 12 OS samples with bulk transcriptomics and high-dimensional Weighted Gene Coexpression Network Analysis (hdWGCNA).. The identified core metabolic regulator was comprehensively validated in vitro using functional assays and Seahorse extracellular flux analysis in MG-63 and U2OS cells.

    Concentration Assay:

    Article Title: Extracellular vesicle-mediated preconditioning of osteoblasts by ER+ breast cancer cells: implications for the bone metastatic niche
    Article Snippet: .. Human hFOB 1.19 osteoblast cells (OBs) (ATCC, CRL-3602) were cultured in DMEM/Ham’s F-12 media (Gibco 11039021) supplemented with 10% FBS (ATCC, 3020) and G418 at a final concentration of 0.3 mg/mL (Gibco, 10131035) per ATCC guidelines (osteogenic media). .. Human T47D breast cancer cells (ATCC, HTB-133) were cultured in RPMI-1640 medium (ATCC, 30–2001).

    Incubator:

    Article Title: Bone organoid–derived extracellular vesicles promote angiogenesis and osteogenesis in vitro
    Article Snippet: .. The human osteoblast cell line hFOB1.19 was purchased from ATCC and cultured in DMEM containing 10% exosome-free fetal bovine serum and 1% penicillin– streptomycin (10,000 U/mL) at 37 °C in a 5% CO2 incubator. ..



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