phusion reaction buffer (New England Biolabs)
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Phusion Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hf+buffer/Phusion+HF+Buffer/us12612449-755-38-41
Average 96 stars, based on 1641 article reviews
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Polymerase Chain Reaction:Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD Article Snippet: .. PCR was performed with Phusion HF DNA polymerase with 5 × Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients Article Snippet: .. Each 20 μl PCR reaction contained 25 ng of gel-extracted product, 0.2 μM of each primer, 0.3 mM dNTP, 1 × Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD. Article Snippet: .. PCR was performed with Phusion HF DNA polymerase with 5 × other:Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation Article Snippet: 5x Phusion Reaction Buffer , Article Title: HIV-1 antibodies Article Snippet: The plate was incubated at room temperature for 1 h, washed then developed by adding SureBlue TMB (Australian Biosearch) according to the manufacturer's instructions. Amplification:Article Title: Powassan Virus LB Neurovirulence and Lethality is Determined by Envelope Protein Domain III Residues Article Snippet: POWV cDNA was amplified into six individual POWV DNA fragments: initial denaturation 98°C for 30 s; 32 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C with 30 s per kb; and a final extension at 72°C for 5 min. Fragments were gel purified and isolated using Monarch DNA Gel Extraction kit (New England Biolabs). .. DNA fragments 1-5 and linker (0.09 pmol each) were CPER amplified in a 25-μL reaction containing 200 μM of dNTPs, Incubation:Article Title: DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity Article Snippet: Then, an equal amount of isopropanol + 1.5 μl of GlycoBlue coprecipitant (Invitrogen, #AM9515) was added to the supernatant and incubated for 2 h at −20 °C before precipitation by centrifugation (20 000 × g for 30 min at 4 °C). .. DNA was resuspended in 5 μl of nuclease-free water (Ambion, #AM9937) and incubated with 1 mM dNTPs, 1× |
