rcutsmart buffer (New England Biolabs)
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New England Biolabs
rcutsmart buffer
Rcutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hf+buffer/AgeI-HF/pmc13125632-250-30-32
Average 97 stars, based on 901 article reviews
Rcutsmart Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hf+buffer/AgeI-HF/pmc13125632-250-30-32
Average 97 stars, based on 901 article reviews
rcutsmart buffer - by Bioz Stars,
2026-11
97/100 stars
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Amplification:Article Title: Modeling Protein Diffusion Across ER-Nuclear Envelope Junctions Reveals Efficient Transport via Simple Diffusion. Article Snippet: .. The amplified fragment was then inserted into the moxGFP-KDEL plasmid using Article Title: A massively parallel reporter assay of MECP2cis-regulatory elements reveals genetic candidates for male-biased autism. Article Snippet: .. The amplicon was then cloned into the vector pLS-SceI (Addgene 137725), prepared by linearizing with Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction. Article Snippet: .. For this, Plasmid Preparation:Article Title: Modeling Protein Diffusion Across ER-Nuclear Envelope Junctions Reveals Efficient Transport via Simple Diffusion. Article Snippet: .. The amplified fragment was then inserted into the moxGFP-KDEL plasmid using Article Title: A massively parallel reporter assay of MECP2cis-regulatory elements reveals genetic candidates for male-biased autism. Article Snippet: .. The amplicon was then cloned into the vector pLS-SceI (Addgene 137725), prepared by linearizing with Article Title: Mitotic bypass and continued endocycling promote cancer cell survival after genotoxic chemotherapy Article Snippet: For inducible p21 overexpression, Gateway donor pDONR223_CDKN1A_WT (a gift from Jesse Boehm & William Hahn & David Root; Addgene plasmid #82201) was recombined with Gateway destination pLIX_403 (a gift from David Root; Addgene plasmid #41395) using Gateway LR Clonase II (Invitrogen #11791020) to generate the CDKN1A expression clone. .. For inducible p21 knockdown, Tet-pLKO-Neo [59] (a gift from Dmitri Wiederschain; Addgene plasmid # 21916) was digested with Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction. Article Snippet: .. For this, Article Title: Harnessing glucocorticoid receptor antagonism to enhance the efficacy of cardiac regenerative growth factors and cytokines. Article Snippet: Myocardial injuries lead to cardiomyocyte loss and heart failure.. Endogenous glucocorticoids, via the glucocorticoid receptor (GR), limit cardiomyocyte regeneration.. Here we show that glucocorticoids suppress mammalian (murine) cardiomyocyte proliferative response to regenerative growth factors and cytokines. Clone Assay:Article Title: A massively parallel reporter assay of MECP2cis-regulatory elements reveals genetic candidates for male-biased autism. Article Snippet: .. The amplicon was then cloned into the vector pLS-SceI (Addgene 137725), prepared by linearizing with Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into pLKO.1puro (Addgene, #8453), which was sequentially digested with Eco RI-HF and Knockdown:Article Title: Mitotic bypass and continued endocycling promote cancer cell survival after genotoxic chemotherapy Article Snippet: For inducible p21 overexpression, Gateway donor pDONR223_CDKN1A_WT (a gift from Jesse Boehm & William Hahn & David Root; Addgene plasmid #82201) was recombined with Gateway destination pLIX_403 (a gift from David Root; Addgene plasmid #41395) using Gateway LR Clonase II (Invitrogen #11791020) to generate the CDKN1A expression clone. .. For inducible p21 knockdown, Tet-pLKO-Neo [59] (a gift from Dmitri Wiederschain; Addgene plasmid # 21916) was digested with shRNA:Article Title: Mitotic bypass and continued endocycling promote cancer cell survival after genotoxic chemotherapy Article Snippet: For inducible p21 overexpression, Gateway donor pDONR223_CDKN1A_WT (a gift from Jesse Boehm & William Hahn & David Root; Addgene plasmid #82201) was recombined with Gateway destination pLIX_403 (a gift from David Root; Addgene plasmid #41395) using Gateway LR Clonase II (Invitrogen #11791020) to generate the CDKN1A expression clone. .. For inducible p21 knockdown, Tet-pLKO-Neo [59] (a gift from Dmitri Wiederschain; Addgene plasmid # 21916) was digested with Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into pLKO.1puro (Addgene, #8453), which was sequentially digested with Eco RI-HF and Construct:Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens Article Snippet: .. To clone the downstream lentiMPRA construct, we first digested the lentiviral backbone ( table S4 ) with Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into pLKO.1puro (Addgene, #8453), which was sequentially digested with Eco RI-HF and Gel Purification:Article Title: Systematic mapping of enhancers, silencers, and topological elements with prime editing deletion screens Article Snippet: .. To clone the downstream lentiMPRA construct, we first digested the lentiviral backbone ( table S4 ) with Expressing:Article Title: TRAM promotes Toll-like-receptor-free myddosome signal transduction. Article Snippet: .. For this, Over Expression:Article Title: CircACVR2A(2-4) regulates stem cell pluripotency by uncoupling CDK-activating kinase functions. Article Snippet: .. Short hairpin RNA and circRNA overexpression constructs All human RNA-targeting shRNAs were cloned into pLKO.1puro (Addgene, #8453), which was sequentially digested with Eco RI-HF and |