5x phusion reaction buffer (New England Biolabs)

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New England Biolabs 5x phusion reaction buffer
5x Phusion Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hf+buffer/Phusion+HF+Buffer/pmc13018877-52-0-5
Average 96 stars, based on 1637 article reviews

5x phusion reaction buffer - by Bioz Stars, 2026-09

96/100 stars

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Polymerase Chain Reaction:

Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD
Article Snippet: .. PCR was performed with Phusion HF DNA polymerase with 5 × Phusion GC reaction buffer (New England Biolabs). .. Each reaction contained 10 μl of buffer, 4 μl of deoxynucleotide triphosphate (dNTPs; final concentration of 200 μM), 5 μl of a 10 μM forward and reverse primer mix, 50 ng of template, 10 μl of 3 M betaine monohydrate (Sigma-Aldrich), 1 μl of dimethyl sulfoxide, 0.5 μl (1 U) of polymerase, and nuclease-free water to 50 μl.

Article Title: HIV-1 antibodies
Article Snippet: Antibodies were eluted from chromatography columns using 50 mM glycine (pH=2.7) and immediately neutralised by addition of 1/10 volume of 1 M Tris (pH=8.0) before being buffer exchanged into PBS, concentration using Amicon 50 kDa spin membranes (Millipore) and sterilization using 0.22 μm filters. .. Generation of HIV-1 AD8 pseudovirus mutant: Specific amino acid changes to HIV-1 AD8 gp160 Env were introduced using the following PCR reaction set up: 100 ng Full-length AD8 gp160 Env plasmid, 5% Dimethyl sulfoxide (DMSO), 10 μl 5× Phusion Reaction buffer (New England BioLabs), 1 μl dNTP mix (10 mM, Promega), 3 U Phusion HF DNA Polymerase (New England BioLabs, (MEL-2000 units/ml)), 0.5 μl from each forward and Reverse primer (20 μM) (Table 4) and nuclease-free H2O up to the total volume of 50 μl. .. The plate was incubated at room temperature for 1 h, washed then developed by adding SureBlue TMB (Australian Biosearch) according to the manufacturer's instructions.

Article Title: Longitudinal Characterization of Gingival Microbiota Following Periodontally Accelerated Osteogenic Orthodontics Surgery in Skeletal Class III Orthognathic Patients
Article Snippet: .. Each 20 μl PCR reaction contained 25 ng of gel-extracted product, 0.2 μM of each primer, 0.3 mM dNTP, 1 × Phusion HF buffer, and 0.4 U Phusion high-fidelity DNA polymerase (NEB). .. PCR products were purified with the QIAquick PCR purification kit (Qiagen) and sequenced on the Illumina Nova PE150 platform.

Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD.
Article Snippet: .. PCR was performed with Phusion HF DNA polymerase with 5 × Phusion GC reaction buffer (New England Biolabs). .. Each reaction contained 10 μl of buffer, 4 μl of deoxynucleotide triphosphate (dNTPs; final concentration of 200 μM), 5 μl of a 10 μM forward and reverse primer mix, 50 ng of template, 10 μl of 3 M betaine monohydrate (Sigma- Aldrich), 1 μl of dimethyl sulfoxide, 0.5 μl (1 U) of polymerase, and nuclease- free water to 50 μl.

other:

Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation
Article Snippet: 5x Phusion Reaction Buffer , NEB , Cat# B0518S.

Mutagenesis:

Plasmid Preparation:

Amplification:

Article Title: Powassan Virus LB Neurovirulence and Lethality is Determined by Envelope Protein Domain III Residues
Article Snippet: POWV cDNA was amplified into six individual POWV DNA fragments: initial denaturation 98°C for 30 s; 32 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C with 30 s per kb; and a final extension at 72°C for 5 min. Fragments were gel purified and isolated using Monarch DNA Gel Extraction kit (New England Biolabs). .. DNA fragments 1-5 and linker (0.09 pmol each) were CPER amplified in a 25-μL reaction containing 200 μM of dNTPs, Phusion polymerase GC reaction buffer, and 0.5 μL Phusion polymerase (New England Biolabs). ..

Incubation:

Article Title: DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activity
Article Snippet: Then, an equal amount of isopropanol + 1.5 μl of GlycoBlue coprecipitant (Invitrogen, #AM9515) was added to the supernatant and incubated for 2 h at −20 °C before precipitation by centrifugation (20 000 × g for 30 min at 4 °C). .. DNA was resuspended in 5 μl of nuclease-free water (Ambion, #AM9937) and incubated with 1 mM dNTPs, 1× Phusion buffer, 1 U of high-fidelity Phusion polymerase (New England BioLabs, #M0530, 2000 U/mL), and 1.25 μM of OLIGO-Reverse primer ( ) in a final volume of 25 μl. ..

Review