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Carl Roth GmbH random hexameric primers
Random Hexameric Primers, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hexanucleotide+random+primers/hexanucleotide+random+primer+mix/pmc06593527-101-14-17
Average 90 stars, based on 1 article reviews
random hexameric primers - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Platelets regulate ischemia-induced revascularization and angiogenesis by secretion of growth factor–modulating factors
Article Snippet: Transcription into cDNA was performed using Random 620 hexamer primer mix (Carl Roth GmbH, Karlsruhe, Germany), MMLV RT (Thermo 621 Fisher Scientific) and RiboLock (Thermo Fisher Scientific).

Article Title: Genetic profiling: Differentiation and identification of hazelnut cultivars (Corylus avellana L.) using RAPD-PCR
Article Snippet: Accepted Manuscript Genetic profiling: Differentiation and identification of hazelnut cultivars (Corylus avellana L.) using RAPD-PCR Christine Felbinger, Florian Kutzsche, Stella Mönkediek, Markus Fischer PII: S0956-7135(19)30380-9 DOI: https://doi.org/10.1016/j.foodcont.2019.106791 Article Number: 106791 Reference: JFCO 106791 To appear in: Food Control Received Date: 28 May 2019 Revised Date: 28 July 2019 Accepted Date: 29 July 2019 Please cite this article as: Felbinger C., Kutzsche F., Mönkediek S. & Fischer M., Genetic profiling: Differentiation and identification of hazelnut cultivars (Corylus avellana L.) using RAPD-PCR, Food Control (2019), doi: https://doi.org/10.1016/j.foodcont.2019.106791.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Rho-family GTPase 1 (Rnd1) is a biomechanical stress-sensitive activator of cardiomyocyte hypertrophy.
Article Snippet: Accepted Manuscript Rho-family GTPase 1 (Rnd1) is a biomechanical stress-sensitive activator of cardiomyocyte hypertrophy Annika Kluge, Ashraf Yusuf Rangrez, Lucia Sophie Kilian, Jost Pott, Alexander Bernt, Robert Frauen, Astrid Rohrbeck, Norbert Frey, Derk Frank PII: S0022-2828(18)30728-4 DOI: https://doi.org/10.1016/j.yjmcc.2019.01.028 Reference: YJMCC 8912 To appear in: Journal of Molecular and Cellular Cardiology Received date: 31 July 2018 Revised date: 29 January 2019 Accepted date: 31 January 2019 Please cite this article as: A. Kluge, A.Y.. Rangrez, L.S.. Kilian, et al., Rho-family GTPase 1 (Rnd1) is a biomechanical stress-sensitive activator of cardiomyocyte hypertrophy, Journal of Molecular and Cellular Cardiology, https://doi.org/10.1016/j.yjmcc.2019.01.028 This is a PDF file of an unedited manuscript that has been accepted for publication.

Article Title: MicroRNA miR-301a is a novel cardiac regulator of Cofilin-2
Article Snippet: Contaminating DNA was digested using DNase I (Thermo Fisher Scientific) and total RNA concentration was measured on NanoDrop spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized using 1μg total RNA with hexanucleotide random-primer-mix (Carl Roth) and SuperScript III cDNA synthesis kit (Thermo Fisher Scientific).

Random Hexamer:

Article Title: The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.
Article Snippet: .. Transcription into cDNA was performed using Random hexamer primer mix (Carl Roth GmbH, Karlsruhe, Germany), MMLV RT (Thermo Fisher Scientific), and RiboLock (Thermo Fisher Scientific). .. Equal amounts of cDNA were applied for qPCR amplification using Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA) and 7900 HT Real-Time PCR System (Applied Biosystems).

Article Title: AHR Signaling Dampens Inflammatory Signature in Neonatal Skin γδ T Cells
Article Snippet: For the visualization of the GSEA-results, the EnrichmentMap-Plugin [ ] in Cytoscape [ ] was used according to the protocol of Reimand et al. 2019 [ ]. .. RNA from sorted cells was extracted from frozen cell pellets using the NucleoSpin RNA XS kit (Macherey Nagel) according to the manufacturer’s protocol. cDNA was prepared with random hexamer primers (Carl Roth), dithiothreitol (Sigma-Aldrich), RiboLock (ThermoFisher Scientific) and M-MLV reverse transcriptase, (Promega, Madison, WI, USA). qPCR was performed in a Qiagen rotor gene using Sybr-green master mix (Biorad, Hercules, CA, USA). ..

Reverse Transcription:

Article Title: AHR Signaling Dampens Inflammatory Signature in Neonatal Skin γδ T Cells
Article Snippet: For the visualization of the GSEA-results, the EnrichmentMap-Plugin [ ] in Cytoscape [ ] was used according to the protocol of Reimand et al. 2019 [ ]. .. RNA from sorted cells was extracted from frozen cell pellets using the NucleoSpin RNA XS kit (Macherey Nagel) according to the manufacturer’s protocol. cDNA was prepared with random hexamer primers (Carl Roth), dithiothreitol (Sigma-Aldrich), RiboLock (ThermoFisher Scientific) and M-MLV reverse transcriptase, (Promega, Madison, WI, USA). qPCR was performed in a Qiagen rotor gene using Sybr-green master mix (Biorad, Hercules, CA, USA). ..

Article Title: Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects
Article Snippet: .. Reverse transcription was performed with 50 ng of isolated RNA using the Omniscript reverse transcriptase (Qiagen, Germany) and random hexanucleotide primer (Carl Roth, Austria). .. Quantitative PCR (qPCR) was performed using the Maxima SyBr Green/ROX qPCR master mix (Thermo Scientific, Austria).

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: Total RNA was isolated using the RNeasy Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions. .. 500 ng of total RNA was reverse transcribed into cDNA with Omniscript (Qiagen) using random hexameric primers (Carl Roth, Karlsruhe, Germany) according to manufacturer’s instructions. .. Real-time PCR was done on a Rotor-Gene Q (Qiagen) using SYBR Green PCR reagents (Thermo Fisher Scientific).

Real-time Polymerase Chain Reaction:

Article Title: AHR Signaling Dampens Inflammatory Signature in Neonatal Skin γδ T Cells
Article Snippet: For the visualization of the GSEA-results, the EnrichmentMap-Plugin [ ] in Cytoscape [ ] was used according to the protocol of Reimand et al. 2019 [ ]. .. RNA from sorted cells was extracted from frozen cell pellets using the NucleoSpin RNA XS kit (Macherey Nagel) according to the manufacturer’s protocol. cDNA was prepared with random hexamer primers (Carl Roth), dithiothreitol (Sigma-Aldrich), RiboLock (ThermoFisher Scientific) and M-MLV reverse transcriptase, (Promega, Madison, WI, USA). qPCR was performed in a Qiagen rotor gene using Sybr-green master mix (Biorad, Hercules, CA, USA). ..

Isolation:

Article Title: Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects
Article Snippet: .. Reverse transcription was performed with 50 ng of isolated RNA using the Omniscript reverse transcriptase (Qiagen, Germany) and random hexanucleotide primer (Carl Roth, Austria). .. Quantitative PCR (qPCR) was performed using the Maxima SyBr Green/ROX qPCR master mix (Thermo Scientific, Austria).



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