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Enzo Biochem high-yield rna transcript labeling kit (t7
High Yield Rna Transcript Labeling Kit (T7, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hea+beadchip+kit+bioarray+solution/rna+transcript+labeling+kit/pmc02423092-50-8-14
Average 90 stars, based on 1 article reviews
high-yield rna transcript labeling kit (t7 - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Labeling:

Article Title: Infectomic Analysis of Gene Expression Profiles of Human Brain Microvascular Endothelial Cells Infected with Cryptococcus neoformans
Article Snippet: Total RNA was preparedusing TRIZOL reagent (Invitrogen, Calif, USA), and subjectedto isolation of poly(A) + RNA using the Oligotex-dT30 mRNA purification kit (TaKaRa Shuzo Co., Kyoto, Japan) according to the manufacturer's instructions. .. The biotinylated cRNA probe was prepared using the RNA Transcript labeling kit (Enzo Biochem, Farmingdale, NY, USA) according to the manufacturer's instructions. ..

Article Title: Transcriptome analysis of haploid male gametophyte development in Arabidopsis
Article Snippet: Double-stranded cDNA was synthesized using SuperScript Choice System (Life Technologies) with oligo(dT) 24 primer fused to T7 RNA polymerase promoter. .. Biotin-labeled target cRNA was prepared by cDNA in vitro transcription using the BioArray High-Yield RNA Transcript Labeling Kit (Enzo Biochem) in the presence of biotinylated UTP and CTP. ..

Article Title: Sex Differences in Global mRNA Content of Human Skeletal Muscle
Article Snippet: Ten micrograms of purified RNA was reverse transcribed using SuperScript II Reverse Transcriptase and T7-(dT) 24 primer followed by second strand DNA synthesis as per the manufacturer's instructions (Life Technologies, Rockville, MD). .. Contaminants were removed from the samples by phenol-chloroform-isoamyl alcohol extraction, and the clean cDNA was recovered by ethanol precipitation, using a RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), it was further converted into biotin-labeled cRNA, as per manufacturer's instructions. .. Moreover, cRNA was purified using a RNeasy Mini Kit (Quiagen, Life Technologies, Rockville, MD) and fragmented in pieces <200 bases by incubation in fragmentation buffer.

Article Title: The IL-10 and IFN-γ pathways are essential to the potent immunosuppressive activity of cultured CD8 + NKT-like cells
Article Snippet: .. In vitro transcription was performed on the above ds-cDNA using the Enzo RNA transcript Labeling kit. .. Biotin-labeled cRNA was purified by using an RNeasy affinity column (Qiagen), and fragmented randomly to sizes ranging from 35-200 bases by incubating at 94°C for 35 minutes.

Article Title: Gene expression profiling reveals effects of Cimicifuga racemosa (L.) NUTT. (black cohosh) on the estrogen receptor positive human breast cancer cell line MCF-7
Article Snippet: .. From the ds-cDNA template biotin-labeled cRNA was made with the Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics, Farmingdale, NY). .. From the ds-cDNA template biotin-labeled cRNA was made with the Enzo High Yield RNA Transcript labeling kit (Enzo Diagnostics, Farmingdale, NY).

In Vitro Transcription:

Article Title: Transcriptome analysis of haploid male gametophyte development in Arabidopsis
Article Snippet: Double-stranded cDNA was synthesized using SuperScript Choice System (Life Technologies) with oligo(dT) 24 primer fused to T7 RNA polymerase promoter. .. Biotin-labeled target cRNA was prepared by cDNA in vitro transcription using the BioArray High-Yield RNA Transcript Labeling Kit (Enzo Biochem) in the presence of biotinylated UTP and CTP. ..

Article Title: The IL-10 and IFN-γ pathways are essential to the potent immunosuppressive activity of cultured CD8 + NKT-like cells
Article Snippet: .. In vitro transcription was performed on the above ds-cDNA using the Enzo RNA transcript Labeling kit. .. Biotin-labeled cRNA was purified by using an RNeasy affinity column (Qiagen), and fragmented randomly to sizes ranging from 35-200 bases by incubating at 94°C for 35 minutes.

Extraction:

Article Title: Sex Differences in Global mRNA Content of Human Skeletal Muscle
Article Snippet: Ten micrograms of purified RNA was reverse transcribed using SuperScript II Reverse Transcriptase and T7-(dT) 24 primer followed by second strand DNA synthesis as per the manufacturer's instructions (Life Technologies, Rockville, MD). .. Contaminants were removed from the samples by phenol-chloroform-isoamyl alcohol extraction, and the clean cDNA was recovered by ethanol precipitation, using a RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), it was further converted into biotin-labeled cRNA, as per manufacturer's instructions. .. Moreover, cRNA was purified using a RNeasy Mini Kit (Quiagen, Life Technologies, Rockville, MD) and fragmented in pieces <200 bases by incubation in fragmentation buffer.

Ethanol Precipitation:

Article Title: Sex Differences in Global mRNA Content of Human Skeletal Muscle
Article Snippet: Ten micrograms of purified RNA was reverse transcribed using SuperScript II Reverse Transcriptase and T7-(dT) 24 primer followed by second strand DNA synthesis as per the manufacturer's instructions (Life Technologies, Rockville, MD). .. Contaminants were removed from the samples by phenol-chloroform-isoamyl alcohol extraction, and the clean cDNA was recovered by ethanol precipitation, using a RNA Transcript Labeling Kit (Enzo Diagnostics, Farmingdale, NY), it was further converted into biotin-labeled cRNA, as per manufacturer's instructions. .. Moreover, cRNA was purified using a RNeasy Mini Kit (Quiagen, Life Technologies, Rockville, MD) and fragmented in pieces <200 bases by incubation in fragmentation buffer.

other:

Article Title: Anticancer drug clustering in lung cancer based on gene expression profiles and sensitivity database
Article Snippet: Total RNA was used to synthesize double-strand cDNA with ReverTraAce and a T7-(dT)24 primer (Metabion, Germany).

Article Title: Three microarray platforms: an analysis of their concordance in profiling gene expression
Article Snippet: cRNA targets for the Affymetrix arrays were synthesized, labeled, and purified according to vendor's (Affymetrix) instructions.

Article Title: Specific age related signatures in Drosophila body parts transcriptome
Article Snippet: RNA quality was assessed using Agilent's Bioanalyser.



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