Magnetic Beads:Article Title: FAK/SRC-JNK axis promotes ferroptosis via upregulating ACSL4 expression
Article Snippet: Primary antibodies used were anti-FAK (proteintech, Cat#66258-1-Ig), anti-SRC (proteintech, Cat#60315-1-Ig), anti-JNK1 (proteintech, Cat#66210-1-Ig), anti-JNK2 (PTMALO, Cat#PTM-6948), anti-ACSL4 (SANTA CRUZ, Cat#sc365230), anti-SMAD4 (proteintech, Cat#10231-1-AP), anti-SMAD4 (MCE, Cat#HY- P80326 ), anti-Phospho-FAK-Ser708 (Wanleibio, Cat#WL02764), anti-Phospho-SRC-Tyr530 (Wanleibio, Cat#WL02114), anti-Phospho-JNK-Thr183/Tyr185 (Wanleibio, Cat#WL01813), anti-GAPDH (proteintech, Cat#60004-1-Ig). anti-NFATC1 (Wanleibio, Cat#WL01632), anti-NFATC1 (SANTA CRUZ, Cat#sc-7294), anti-NFATC3 (SANTA CRUZ, Cat#sc-8405), anti-ATF2 (SANTA CRUZ, Cat#sc-242), anti-ELK1 (proteintech, Cat#27420-1-AP), anti-ELK1 (PTM BIO, Cat#20042), anti-STAT3 (SANTA CRUZ, Cat#sc-8019), anti-p-ELK1 (SANTA CRUZ, Cat#sc-8406), anti-p-NFATC3 (SANTA CRUZ, Cat#sc-365785), anti-c-Jun (CST, Cat#9165), anti-c-Jun (proteintech, Cat#24909-1-AP), anti-p-c-Jun (SANTA CRUZ, Cat#sc-8322), anti-p-ATF2 (SANTA CRUZ, Cat#sc-8398), anti-HSF1 (SANTA CRUZ, Cat#sc-17757), anti-SLC7A11 (proteintech, Cat#26864-1-AP), anti-GPX4 (abcam, Cat#ab231174), anti-alpha Tubulin (proteintech, Cat#11224-1-AP), anti-ATP1A1 (proteintech, Cat#14418-1-AP), anti-Histone H3 (Wanleibio, Cat#WL0984a), anti-MBOAT1 (proteintech, Cat#25615-1-AP), anti-MBOAT2 (abcepta, Cat#AP17786c), anti-DHODH (proteintech, Cat#14877-1-AP), anti-ALOX15 (abcam Cat#244205), anti-4-Hydroxynonenal (Thermo fisher, Cat# MA5-27570), anti-NCOA4 (proteintech, Cat#10727-1-AP), anti-FTH1 (HUABIO, Cat#ET1705-55) and anti-FTL1 (Affinity, Cat#DF6604), anti-RIP3 (HUABIO cat#HA722183), anti-p-RIP3 (HUABIO cat#HA721428), anti-caspase3 (proteintech, Cat#19677-1-AP), anti-p-MLKL (BOSTER cat# P00535 ), anti-MLKL (proteintech, Cat#66675-1-Ig) Chemical reagents used were erastin (Selleck, Cat#S7242), Defactinib (TargetMOL, Cat#1073154-85-4), Saracatinib (Selleck, Cat#AZDO530), Imidazole ketone erastin (Selleck, Cat#S8877), Kartogenin (Adoop Bioscience, Cat#A12926), DTHIB (TargetMOL, Cat#897326-30-6), HSF1A (TargetMOL, Cat#1196723-93-9), STAT3-IN-1 (TargetMOL, Cat#2059952-75-7), NDMC101 (TargetMOL, Cat#1308631-40-4), BODIPY C11 (Thermo Fisher, Cat# D3861). .. Hematoxylin-Eosin (H&E) HD Constant Dye Kit (Servicebio, Cat# G1076), PI (Sigma-Aldrich, Cat#25535-16-4), Protein A/G AgaroseThermo Fisher, Cat#20421, Protein A/G Magnetic Beads (MCE,Cat#HY-K0202), TRIzol (Thermo Fisher, Cat# 15596026) Xylene (SCRC, Cat# 10023418), Normal Butanol (SCRC, Cat# 100052190), Environmental Friendly Dewaxing Transparent Liquid (Servicebio, Cat# G1128-1L), Paraformaldehyde Fixative (Neutral) (Servicebio, Cat# G1101), Neutral Gum (SCRC, Cat# 10004160), L-Arginine (Sigma, Cat#74-79-3). .. HT1080, B16-F10 and HEK293T were purchased from ATCC.
Staining:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Article Title: Notch Signaling Is Associated with LED Light-Regulated Papilla Regeneration in the Sea Cucumber ( Apostichopus japonicus ).
Article Snippet: .. After paraffin embedding, 5 μm-thick longitudinal tissue sections were prepared and baked at 60 ◦C for 2 h. Hematoxylin–Eosin (HE) Staining: Sections were dewaxed and rehydrated using an environment-friendly dewaxing solution (Servicebio, Cat. No. G1128), treated with high-definition staining pretreatment solution (from HE staining kit, Servicebio, Cat. No. G1076) for 1 min, stained with hematoxylin for 3 min, differentiated with differentiation solution (Servicebio, Cat. No. G1039) for 3–5 s, rinsed with running water for bluing, stained with eosin for 15 s, dehydrated, cleared, and mounted with neutral gum. .. Masson’s Trichrome Staining: After dewaxing and rehydration, sections were incubated with Solution A from the Masson staining kit (Servicebio, Cat. No. G1006) at 65 ◦C for 30 min, stained with a mixture of Solutions B and C for 1 min, incubated with Solution https://doi.org/10.3390/ijms27094105 D for 6 min, stained with Solution F for 20 s, rinsed with 1% glacial acetic acid, dehydrated, cleared, and mounted with neutral gum.
Article Title: Alginate-TEMPO conjugate hydrogel with synergistic antioxidant and angiogenic activities for accelerating wound healing.
Article Snippet: Effective management of cutaneous wounds remains a significant clinical challenge, often exacerbated by excessive reactive oxygen species (ROS) in the wound microenvironment.. This study developed a novel hydrogel for accelerated wound healing based on a covalent conjugate (Alg-T) of sodium alginate, a natural polysaccharide macromolecule, and a ROS scavenger 4-amino-TEMPO.. Two kinds of conjugates with grafting ratios of approximately 3% (Alg-T1) and 8% (Alg-T2) were successfully synthesized.
Immunohistochemical staining:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Western Blot:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Marker:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
RNA Extraction:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Lysis:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Real-time Polymerase Chain Reaction:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
SYBR Green Assay:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
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