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adult ventricular cardiac fibroblasts hcfs  (PromoCell)


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    Structured Review

    PromoCell adult ventricular cardiac fibroblasts hcfs
    Adult Ventricular Cardiac Fibroblasts Hcfs, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcf+medium/Fibroblast+Growth+Medium/pmc10693443-134-2-23
    Average 94 stars, based on 16 article reviews
    adult ventricular cardiac fibroblasts hcfs - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Thoracic Aortic Aneurysm Development Is Dependent on Membrane Type-1 Matrix Metalloproteinase Activity and Abundance
    Article Snippet: The aorta was then cut into approximately ten 2 mm pieces and carefully placed onto the surface of a dry tissue culture flask (T-75, Cat#13-680-65; BD Falcon, Fisher Scientific, Pittsburgh, PA, USA). .. The tissue was allowed to adhere in the absence of medium for 5–10 min, then 10 mL of fibroblast growth medium (Fibroblast Growth Medium, Promocell Cat#C39315, Heidelberg, Germany), containing Fibroblast Growth Supplement, (Promocell Cat#C23010; consisting of 1.0 ng/mL basic fibroblast growth factor, and 5.0 μg/mL insulin at final concentration) and 10% heat-inactivated fetal-calf serum (Invitrogen, Cat# 10082-147, Carlsbad, CA, USA), https://doi.org/10.3390/biom16020237 was carefully added to the flask. ..

    Article Title: Thoracic Aortic Aneurysm Development Is Dependent on Membrane Type-1 Matrix Metalloproteinase Activity and Abundance
    Article Snippet: The aorta was then cut into approximately ten 2 mm pieces and carefully placed onto the surface of a dry tissue culture flask (T-75, Cat#13-680-65; BD Falcon, Fisher Scientific, Pittsburgh, PA, USA). .. The tissue was allowed to adhere in the absence of medium for 5–10 min, then 10 mL of fibroblast growth medium (Fibroblast Growth Medium, Promocell Cat# C39315 , Heidelberg, Germany), containing Fibroblast Growth Supplement, (Promocell Cat# C23010 ; consisting of 1.0 ng/mL basic fibroblast growth factor, and 5.0 μg/mL insulin at final concentration) and 10% heat-inactivated fetal-calf serum (Invitrogen, Cat# 10082-147, Carlsbad, CA, USA), was carefully added to the flask. ..

    Recombinant:

    Article Title: MicroAge mission: experimental design and hardware for a bespoke culture system supporting tissue-engineered skeletal muscle.
    Article Snippet: Unless stated otherwise, all other chemicals used in this study were obtained from Sigma Aldrich, Dorset, UK. .. Human immortalised myoblasts (AB1167_CTL) were routinely maintained in a complete growth medium consisting of PromoCell skeletal muscle basal medium (phenol red-free) supplemented with 20 % (v/v) FBS, 50 g/mL bovine fetuin, 10 ng/mL epidermal growth factor, 1 ng/mL basic fibroblast growth factor, 10 g/mL recombinant human insulin, 0.4 μg/mL dexamethasone, 10 g/ mL gentamicin and 4 mM Lglutamine 19,20. ..

    Cell Culture:

    Article Title: Exploratory Volatilome Profiling of Inflammation in Skin Fibroblasts: A Proof-of-Concept Study
    Article Snippet: .. Primary Normal Human Dermal Fibroblasts (NHDFs) from juvenile foreskin were obtained from PromoCell (Heidelberg, Germany) and cultured in Fibroblast Basal Medium (PromoCell) with added Fibroblast Growth Medium SupplementPack (PromoCell). .. Primary Normal Human Dermal Fibroblasts (NHDFs) from juvenile foreskin were obtained from PromoCell (Heidelberg, Germany) and cultured in Fibroblast Basal Medium (PromoCell) with added Fibroblast Growth Medium SupplementPack (PromoCell).

    Article Title: Antioxidant and Anti-Aging Effects of Porphyra-334 Produced from Saccharomyces cerevisiae in Human Skin Models.
    Article Snippet: .. NHDF (Promocell, Heidelberg, Germany) were cultured in Fibroblast Growth Medium (Promocell) containing supplement mix and 1% penicillin–streptomycin (PS; Gibco, Grand Island, NY, USA). .. HEKa (Gibco) were cultured in Epilife medium (ThermoFisher, Waltham, MA, USA) with Keratinocyte Growth Supplement (HKGS, Gibco) and 1% PS.

    Modification:

    Article Title: From Fiber Architecture to Functional Attachment: A Clinically Relevant, Mechanically Tunable Cardiac Patch.
    Article Snippet: .. At day 8, the media was changed to Advanced Dulbecco ́s Modified Eagle ́s Medium (ADMEM, Gibco) supplemented with 2% KSR, and kept for 72 h. At day 11, cellswere replated on 0.1% gelatin (MerckMilipore) coated plates, and switched to Fibroblast GrowthMedium3 (FGM3, PromoCell) supplemented with 10 μM SB431542 (Sigma–Aldrich), 10 ng/mL of FGF2, and 10 μM Y27. ..



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    Image Search Results


    Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E and F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. (H) Western blot of CXCR1, CXCR2, and α-tubulin in human iCMs after DMSO or erastin treatment. (I) Normalized band (immuno-blotting) intensity of CXCR1 and CXCR2 in (H). All bar graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; NS, not significant by t test.

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet: Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E and F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. (H) Western blot of CXCR1, CXCR2, and α-tubulin in human iCMs after DMSO or erastin treatment. (I) Normalized band (immuno-blotting) intensity of CXCR1 and CXCR2 in (H). All bar graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; NS, not significant by t test.

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Derivative Assay, Cell Culture, Control, Protein Array, Purification, Western Blot

    Cardiac fibroblasts interact with cardiomyocytes through gap junctions to share free iron (A and B) Wild-type mouse heart tissue stained for Fth1 [magenta, (A)] or Ftl [magenta, (B)], with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows: non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C and D) Mouse heart tissue stained for Fth1 [red, (C)] or Ftl [red, (D)], with Pdgfrα (gray), cTnT (green), and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E and F) Mouse heart tissue stained for Fth1 [green, (E)] or Ftl [green, (F)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H and I) Mouse heart section stained for Cx45 [green, (H)] or Cx43 [green, (I)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) after P7 LAD-O. Arrows: potential locations of gap junctions between cardiomyocytes and fibroblasts. (J–L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, gray), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks: HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture; Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. All bar graphs represent mean ± SD. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by t test. Scale bar, 75 μm (A, B, E, F, H), 25 μm (C, D, I, J–L). See also <xref ref-type=Figures S4 and . " width="100%" height="100%">

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet: Cardiac fibroblasts interact with cardiomyocytes through gap junctions to share free iron (A and B) Wild-type mouse heart tissue stained for Fth1 [magenta, (A)] or Ftl [magenta, (B)], with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows: non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C and D) Mouse heart tissue stained for Fth1 [red, (C)] or Ftl [red, (D)], with Pdgfrα (gray), cTnT (green), and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E and F) Mouse heart tissue stained for Fth1 [green, (E)] or Ftl [green, (F)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H and I) Mouse heart section stained for Cx45 [green, (H)] or Cx43 [green, (I)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) after P7 LAD-O. Arrows: potential locations of gap junctions between cardiomyocytes and fibroblasts. (J–L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, gray), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks: HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture; Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. All bar graphs represent mean ± SD. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by t test. Scale bar, 75 μm (A, B, E, F, H), 25 μm (C, D, I, J–L). See also Figures S4 and .

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Staining, Cell Culture, Knockdown, Co-Culture Assay

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet:

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Recombinant, Produced, Virus, Bicinchoninic Acid Protein Assay, Purification, Sequencing, Negative Control, Software