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hypoxanthine, aminopterin, and thymidine (hat) selection medium supplement  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hypoxanthine, aminopterin, and thymidine (hat) selection medium supplement
    Hypoxanthine, Aminopterin, And Thymidine (Hat) Selection Medium Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hat+selective+medium/hypoxanthine++aminopterin++and+thymidine/pm38203331-153-9-17
    Average 90 stars, based on 1 article reviews
    hypoxanthine, aminopterin, and thymidine (hat) selection medium supplement - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: 15-keto-PGE 2 alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factors
    Article Snippet: Hybridomas were selected in complete Dulbecco's modified Eagle’s medium (DMEM) containing hypoxanthine-aminopterin-thymidine (Thermo Scientific) and UltraCruz Hybridoma Cloning Supplement (Santa Cruz, TX).

    Article Title: Identification of PTGR2 inhibitors as a new therapeutic strategy for diabetes and obesity.
    Article Snippet: Hybridomas were selected in complete Dulbecco’s modified Eagle’s medium (DMEM) containing hypoxanthine-aminopterin-thymidine (cat. no. 11067030, Thermo Fisher) and UltraCruz Hybridoma Cloning Supplement (cat. no. sc224479, Santa Cruz) for 12–14 days.

    Article Title: Development of a novel anti-human glypican 5 monoclonal antibody (G 5 Mab-1) for multiple applications
    Article Snippet: We conducted cell-fusion of the harvested splenocytes from LN229/GPC5-immunized mice with P3U1 cells using polyethylene glycol 1500 (PEG1500; Roche Diagnostics, Indianapolis, IN, USA) under heated conditions.

    Article Title: Gold nanoparticle-based lateral flow immunoassay for the repaid and sensitive detection of pyraclostrobin in wheat, apple, Chinese cabbage, and soil samples
    Article Snippet: Pyraclostrobin is widely employed for controlling fungal pathogens.. Given its potential adverse effects on aquatic organisms, beneficial insects, and mammals, there is an urgent need to develop on-site detection methods for monitoring pyraclostrobin residues.. In this study, monoclonal antibodies (mAbs) targeting pyraclostrobin were developed, and a reliable structure of the mAb 4B4C3 was predicted using AlphaFold 3.

    Produced:

    Article Title: Development of specific anti-mouse atypical chemokine receptor 4 monoclonal antibodies
    Article Snippet: Two five-week-old Sprague-Dawley rats (CLEA Japan, Tokyo, Japan) were intraperitoneally immunized with 100 μg of the KLH-conjugated mACKR4 peptide (mACKR4-KLH) with Alhydrogel adjuvant 2 % (InvivoGen). .. After three additional weekly immunizations (100 μg/rat) and final booster immunizations (100 μg/rat), hybridomas were produced and cultured in the medium containing hypoxanthine, aminopterin, and thymidine (HAT; Thermo Fisher Scientific Inc., Waltham, MA), 10 % FBS, and 5 % BriClone (NICB, Dublin, Ireland). ..

    Cell Culture:

    Article Title: Development of specific anti-mouse atypical chemokine receptor 4 monoclonal antibodies
    Article Snippet: Two five-week-old Sprague-Dawley rats (CLEA Japan, Tokyo, Japan) were intraperitoneally immunized with 100 μg of the KLH-conjugated mACKR4 peptide (mACKR4-KLH) with Alhydrogel adjuvant 2 % (InvivoGen). .. After three additional weekly immunizations (100 μg/rat) and final booster immunizations (100 μg/rat), hybridomas were produced and cultured in the medium containing hypoxanthine, aminopterin, and thymidine (HAT; Thermo Fisher Scientific Inc., Waltham, MA), 10 % FBS, and 5 % BriClone (NICB, Dublin, Ireland). ..

    HAT Assay:

    Article Title: Development of specific anti-mouse atypical chemokine receptor 4 monoclonal antibodies
    Article Snippet: Two five-week-old Sprague-Dawley rats (CLEA Japan, Tokyo, Japan) were intraperitoneally immunized with 100 μg of the KLH-conjugated mACKR4 peptide (mACKR4-KLH) with Alhydrogel adjuvant 2 % (InvivoGen). .. After three additional weekly immunizations (100 μg/rat) and final booster immunizations (100 μg/rat), hybridomas were produced and cultured in the medium containing hypoxanthine, aminopterin, and thymidine (HAT; Thermo Fisher Scientific Inc., Waltham, MA), 10 % FBS, and 5 % BriClone (NICB, Dublin, Ireland). ..

    Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
    Article Snippet: After several additional immunizations of PODXLec, the spleen cells were harvested and fused with P3U1 cells using PEG1500 (Roche Diagnostics, Indianapolis, IN, USA). .. Hybridomas were grown in RPMI-1640 medium, including L-glutamine with hypoxanthine, aminopterin, and thymidine (HAT) selection medium supplement (Thermo Fisher Scientific Inc.). ..

    Selection:

    Article Title: Overcoming antibody-resistant SARS-CoV-2 variants with bispecific antibodies constructed using non-neutralizing antibodies
    Article Snippet: The procedure included three additional immunization procedures (100 μg/mouse), followed by a final booster i.p. injection (100 μg/mouse) 2 days before harvesting the spleen cells, which were subsequently fused with P3U1 cells using polyethylene glycol 1500 (PEG1500; Roche Diagnostics; Indianapolis, IN, USA). .. Hybridomas were grown in RPMI medium supplemented with hypoxanthine, aminopterin, and thymidine for selection (Thermo Fisher Scientific). ..

    Article Title: A Cancer-Specific Monoclonal Antibody against Podocalyxin Exerted Antitumor Activities in Pancreatic Cancer Xenografts.
    Article Snippet: After several additional immunizations of PODXLec, the spleen cells were harvested and fused with P3U1 cells using PEG1500 (Roche Diagnostics, Indianapolis, IN, USA). .. Hybridomas were grown in RPMI-1640 medium, including L-glutamine with hypoxanthine, aminopterin, and thymidine (HAT) selection medium supplement (Thermo Fisher Scientific Inc.). ..

    Article Title: Establishment of a high-affinity anti-mouse CXCR5 monoclonal antibody for flow cytometry
    Article Snippet: The CXC chemokine receptor 5 (CXCR5) is a member of the G protein-coupled receptor family that is highly expressed in B cells and a subset of T cells, such as T follicular helper cells.. Various types of cancers, including non-small cell lung cancer, breast cancer, and prostate cancer, also express CXCR5.. Therefore, antibodies that specifically bind to CXCR5 could be useful for clarification of the mechanisms of cancer progression.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Establishment of a high-affinity anti-mouse CXCR5 monoclonal antibody for flow cytometry
    Article Snippet: The CXC chemokine receptor 5 (CXCR5) is a member of the G protein-coupled receptor family that is highly expressed in B cells and a subset of T cells, such as T follicular helper cells.. Various types of cancers, including non-small cell lung cancer, breast cancer, and prostate cancer, also express CXCR5.. Therefore, antibodies that specifically bind to CXCR5 could be useful for clarification of the mechanisms of cancer progression.

    Flow Cytometry:

    Article Title: Establishment of a high-affinity anti-mouse CXCR5 monoclonal antibody for flow cytometry
    Article Snippet: The CXC chemokine receptor 5 (CXCR5) is a member of the G protein-coupled receptor family that is highly expressed in B cells and a subset of T cells, such as T follicular helper cells.. Various types of cancers, including non-small cell lung cancer, breast cancer, and prostate cancer, also express CXCR5.. Therefore, antibodies that specifically bind to CXCR5 could be useful for clarification of the mechanisms of cancer progression.



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