gus staining solution (Gold Biotechnology Inc)

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Gold Biotechnology Inc gus staining solution
<t>Histochemical</t> <t>staining</t> of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to <t>GUS,</t> thereby producing a single fusion protein). Scale bars are 5 mm.

<t>Histochemical</t> <t>staining</t> of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to <t>GUS,</t> thereby producing a single fusion protein). Scale bars are 5 mm.

Gus Staining Solution, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gus+staining+solution/L-Xylose/pmc12845159-211-19-46
Average 95 stars, based on 1 article reviews

gus staining solution - by Bioz Stars, 2026-11

95/100 stars

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1) Product Images from "Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya"

Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya

Journal: Plants

doi: 10.3390/plants15020209

Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

Figure Legend Snippet: Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

Techniques Used: Staining, Construct, Expressing, Sequencing

other:

Article Title: Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis.
Article Snippet: Cultures were incubated for 3 h with 200 μM Biotin (Sigma, B4501) with shaking at 37 °C.

Incubation:

Article Title: Identification of a GA-Related Cis-Element Regulating Male Peduncle Elongation in Papaya
Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K3FE(CN)6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

Article Title: Membrane-associated effluxosomes coordinate multi-metal resistance in Mycobacterium tuberculosis
Article Snippet: Lysis was performed by mechanical disruption 3 × 45 s (Biospec Minibeadbeater) with 5 min icing between runs. .. For biotinylated protein capture, protein lysates were mixed with 80 μL of Streptavidin-Agarose (GoldBio, S-105-10) prewashed 3X in 1 ml TBS and incubated rotating at 4 °C for 18 h. Agarose beads were collected at 250× g for 1 min at 4 °C and washed 2xs with 1 ml RIPA/SDS (TBS + 0.1% Triton X-100, 0.1% SDS, and 0.5% sodium deoxycholate) followed by four washes with TBS. .. Pooled supernatants were mixed with 150 μL of 10x RIPA buffer detergents (TBS + 10% Triton X-100, 1% SDS, and 5% sodium deoxycholate), mixed by inversion, and centrifuged at 20,000× g . The supernatant was filtered twice through EMD Millipore TM Ultrafree TM -CL Centrifugal Filter Devices with Durapore TM Membrane to remove any residual M. tuberculosis .

Article Title: Motor learning and dopamine-dependent striatal synaptic plasticity are controlled by astrocytic MEGF10
Article Snippet: After fixation, brains were obtained and postfixated overnight (4% PFA, 4 °C), followed by incubation in 30% sucrose (Sigma-Aldrich) in DPBS for at least 48 h. After, the brain was embedded in an OCT compound (Leica) and cryo-sectioned into 30 μm slices. .. Brain slices were incubated in blocking solution (4% bovine serum albumin, GoldBio; 0.3% Triton X-100, Sigma-Aldrich; in DPBS) for an hour at room temperature, followed by incubation in primary antibody solution (48 h, 4 °C). .. Primary antibodies were appropriately diluted in blocking solution (rabbit anti-S100β (1:500), Abcam #ab52642; guinea pig anti-S100β (1:500), Synaptic Systems #287004; rabbit anti-IBA1 (1:500), WAKO #019-19741; rabbit anti-tRFP (1:500), Evrogen #AB233; rat anti-mCherry (1:500), Invitrogen # M11217 ; chicken anti-GFP (1:2000), Aves Labs #GFP-1020; rabbit anti-c-Fos (1:500), Cell signaling technology #2250S; rabbit anti-HA (1:500), Cell signaling technology #3724; chicken anti-HA (1:500), Sigma-Aldrich #AB3254; sheep anti-TH (1:500), Sigma-Aldrich #AB1542; rabbit anti-TH (1:500), Sigma-Aldrich #AB152).

Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya
Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

Article Title: ZINC-FINGER MOTIFS UNIQUE TO ARABIDOPSIS THALIANA PARALOGS RPA1C AND RPA1E ARE REQUIRED FOR RPA-DEPENDENT DNA REPAIR
Article Snippet: .. Following fixation, plants were incubated in 50 mM NaPO 4 , pH 7.2, 0.5 mM K 3 Fe(CN) 6 , 0.5 mM K 4 Fe(CN) 6 , 10 mM EDTA, 0.01% Trition X-100, and 2 mM X-Gluc (Gold Biotechnology, St. Louis, MO, USA) overnight at 37°C. ..

Staining:

Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya
Article Snippet: .. Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight. ..

Blocking Assay:

Luciferase:

Article Title: Pathogen effector disarms circadian-immune crosstalk by targeting TCP14, a dual regulator of clock and defense.
Article Snippet: .. Arabidopsis seedlings of luciferase reporter grown under 12 h light/12 h dark condition at 22◦C for 6 days were transferred to either constant light or diurnal condition and sprayed with 2.5 mM luciferin (Gold Biotechnology, LUCK-1G) in 0.02% Triton X-100 (Figures 1A, S1B, S3F, and S3G). ..

Lysis:

Article Title: The redundant protein synthesis gene Aimp1 challenges the canonical inverse relationship between translation and autophagy.
Article Snippet: .. After 4 hours of exposure to Chlo or BafA1, HAP1 cells were lysed in lysis buffer containing 50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1.0% IGE-PAL, 0.5% Triton-X100, protease inhibitor cocktail (GoldBio, GB331-5), and Simple StopTM 1 Phosphatase Inhibitor Cocktail (GoldBio, GB-450-1). .. After 4 hours of exposure to Chlo or BafA1, HAP1 cells were lysed in lysis buffer containing 50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1.0% IGE-PAL, 0.5% Triton-X100, protease inhibitor cocktail (GoldBio, GB331-5), and Simple Stop 1 Phosphatase Inhibitor Cocktail (GoldBio, GB-450-1).

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