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optimal single guide rna sgrna target sequences  (Benchling Inc)

 
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    Structured Review

    Benchling Inc optimal single guide rna sgrna target sequences
    Optimal Single Guide Rna Sgrna Target Sequences, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/guide+sequence/guide+rna/pm42167230-305-5-14
    Average 86 stars, based on 1 article reviews
    optimal single guide rna sgrna target sequences - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors
    Article Snippet: .. To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’-caccgCAGGACGCTGCGCACAACAT-3’ and 5’-aaacATGTTGTGCGCAGCGTCCTGc-3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961).

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors.
    Article Snippet: .. To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’- caccgCAGGACGCTGCGCACAACAT -3’ and 5’- aaacATGTTGTGCGCAGCGTCCTGc -3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961).

    Synthesized:

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors
    Article Snippet: .. To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’-caccgCAGGACGCTGCGCACAACAT-3’ and 5’-aaacATGTTGTGCGCAGCGTCCTGc-3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961).

    Article Title: Degraders of the dengue virus capsid protein exhibit differentiated pharmacology relative to capsid inhibitors.
    Article Snippet: .. To generate plasmids for CRBN knockout, the guide RNA sequences were designed using Benchling, and oligos were synthesized from Integrated DNA Technology (IDT). .. The oligos 5’- caccgCAGGACGCTGCGCACAACAT -3’ and 5’- aaacATGTTGTGCGCAGCGTCCTGc -3’ were cloned into pLentiCRISPRV2 (Addgene, Plasmid #52961).

    Article Title: FGF4 initiates X-chromosome inactivation via activating YY1 and prompting loss of pluripotency factors.
    Article Snippet: .. Using the online guide designer Benchling, we acquired and synthesized a small guide RNA (sgRNA). sgRNAs were constructed to pSpCas9n(BB)-2A-Puro vector (PX462, purchased from Addgene), at BbsI sites. .. And transfected into PGK12.1 using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

    Software:

    Article Title: Epigenetic editing of Cartpt promotes acquisition and extinction of cocaine memory
    Article Snippet: .. Cartpt guide RNA at -205 base pairs relative to TSS was designed using Benchling (Biology Software) and IDT CRISPR-Cas9 guide RNA design checker (Integrated DNA Technologies). .. All DNA fragments were purchased from IDT (Integrated DNA Technologies).

    CRISPR:

    Article Title: Epigenetic editing of Cartpt promotes acquisition and extinction of cocaine memory
    Article Snippet: .. Cartpt guide RNA at -205 base pairs relative to TSS was designed using Benchling (Biology Software) and IDT CRISPR-Cas9 guide RNA design checker (Integrated DNA Technologies). .. All DNA fragments were purchased from IDT (Integrated DNA Technologies).

    Article Title: Generation of human nucleus basalis organoids with functional nbM-cortical cholinergic projections in transplanted assembloids.
    Article Snippet: .. Generation of CHAT-tdTomato hiPSC line using CRISPR-Cas9 genome editing To generate the CHAT-tdTomato hiPSC line, guide RNA targeting the first 100 bp of the 3 ′ homology arm was designed using Zhang laboratory (https://zlab.bio/guide-design-resources and https://www.benchling.com/). ..

    Clone Assay:

    Article Title: Endocytic evasion confers resistance to antibody-drug conjugates therapy in cancer.
    Article Snippet: In brief Enfortumab vedotin, a NECTIN4targeting antibody-drug conjugate (ADC), is in clinical use for urothelial cancer.. Using patient specimens and preclinical models, Wang et al. identify an endocytic trafficking defect that limits ADC uptake and efficacy and propose a strategy to overcome this resistance.. This trafficking-based mechanism may also underlie resistance to other ADCs in the clinic.

    Plasmid Preparation:

    Article Title: Endocytic evasion confers resistance to antibody-drug conjugates therapy in cancer.
    Article Snippet: In brief Enfortumab vedotin, a NECTIN4targeting antibody-drug conjugate (ADC), is in clinical use for urothelial cancer.. Using patient specimens and preclinical models, Wang et al. identify an endocytic trafficking defect that limits ADC uptake and efficacy and propose a strategy to overcome this resistance.. This trafficking-based mechanism may also underlie resistance to other ADCs in the clinic.

    Article Title: FGF4 initiates X-chromosome inactivation via activating YY1 and prompting loss of pluripotency factors.
    Article Snippet: .. Using the online guide designer Benchling, we acquired and synthesized a small guide RNA (sgRNA). sgRNAs were constructed to pSpCas9n(BB)-2A-Puro vector (PX462, purchased from Addgene), at BbsI sites. .. And transfected into PGK12.1 using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.

    Construct:

    Article Title: FGF4 initiates X-chromosome inactivation via activating YY1 and prompting loss of pluripotency factors.
    Article Snippet: .. Using the online guide designer Benchling, we acquired and synthesized a small guide RNA (sgRNA). sgRNAs were constructed to pSpCas9n(BB)-2A-Puro vector (PX462, purchased from Addgene), at BbsI sites. .. And transfected into PGK12.1 using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.



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