minknow gui software (Oxford Nanopore)
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Minknow Gui Software, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gui+software/minknow+gui+software/pmc08248633-65-26-29
Average 90 stars, based on 1 article reviews
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Sequencing:Article Title: Isolation and characterization of a novel Wolbachia bacteriophage from Allonemobius socius crickets in Missouri Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using Article Title: Isolation and characterization of a novel bacteriophage WO from Allonemobius socius crickets in Missouri Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using Article Title: Isolation and characterization of a novel bacteriophage WO from Allonemobius socius crickets in Missouri. Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using Software:Article Title: Isolation and characterization of a novel Wolbachia bacteriophage from Allonemobius socius crickets in Missouri Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using Article Title: Proof of Concept Study: Comparability of Microbiome Diversity in Self- and Physician-Collected HPV-Positive and HPV-Negative Cervicovaginal Samples Article Snippet: Next, the library was incubated with Library Loading Beads, the mixture was added to Flow Cell version R.9.4, and sequenced using a MinIon sequencer for approximately 4–6 h (Oxford Nanopore Technologies—ONT, Oxford, UK). .. The nanopore electrical signal was converted into nucleotides with Article Title: Isolation and characterization of a novel bacteriophage WO from Allonemobius socius crickets in Missouri Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using Article Title: Isolation and characterization of a novel bacteriophage WO from Allonemobius socius crickets in Missouri. Article Snippet: Library was prepared according to Oxford Nanopore’s 1D Genomic DNA Ligation Protocol (Version GDE_9063_v109_revA) using the LSK-109 Ligation Sequencing Kit (Oxford Nanopore Technologies, Cambridge, England) with DNA fragments of all sizes purified using the Short Fragment Buffer. .. 60 μL of library containing 12 μL genomic DNA was loaded as input into the flow cell and the sequencing reaction run for 20 hours using |
![a, b . Epifluorescence-based widefield imaging of CD8 + T-cells interacting with a monolayer of Lo U-2 OS WT (a) and Lo U-2 OS ICAM-1 (b) cells presenting N-Med ImmTACs. White arrowheads indicate the first detectable close-contacts marked by accumulation of fluorescent CD58-HaloTag expressed by the U-2 OS cells. Images are displayed as maximum-intensity projections. Scale bars, 5 µm. c . Fraction of CD8 + T-cells forming close contacts with N-Med ImmTAC-presenting U-2 OS cells after 5’ (n=3 videos). Error bars show SD. Data were compared using a Welch t test. d . Killing of U-2 OS cells by CD8 + T-cells over time measured using <t>Incucyte</t> imaging. Killing assays were performed using CD8 + T-cells from three donors, in triplicate. Error bars show SEM. Data for Lo U-2 OS WT and Lo U-2 OS ICAM-1 cells at selected time points (t = 3, 6, 9 h) were compared using a Wilcoxon signed-rank test. e . N-Med EC 50 values measured in co-cultures of CD8 + T-cells with U-2 OS WT and U-2 OS ICAM-1 cells. EC 50 values were determined as in Fig. 1; see also Fig. S4. Error bars correspond to the 95% CI. EC 50 values were compared using an F-test of normalised data. Three co-cultures were performed as biological repeats, with measurements in triplicate. Data were collected using CD8 + T-cells from at least two donors. f-n . Image-based analysis of CD8 + T-cells interacting with Lo U-2 OS WT and Lo U-2 OS ICAM-1 cells [n=6 videos each with >20 interacting T cells; comparisons are made with T cells interacting with non ImmTAC-presenting U-2 OS cells (Fig. 2e-g)]. f, g . Average number of close contacts formed per T cell versus time (f) and 5’ after detecting the first close contact (g). h, i . Average summed area of close contacts per T cell versus time (h) and 5’ after detecting the first close contact (i). j, k . Average areas (j) and diameters (k) of all close contacts l . Average area of individual close contacts versus time. m, n . Average cumulative area of the target-cell surface scanned by a single T cell versus time (m) and 5’ after detecting the first close contact (n). Data were compared using a Kruskal-Wallis test and post-hoc Dunn’s test for multiple comparisons. Violin plots indicate median and quartiles. Imaging experiments were performed using CD8 + T-cells from at least two donors.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_15/10__1101_slash_2025__08__29__673015/10__1101_slash_2025__08__29__673015___F3.large.jpg)