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Promega gst/halotag coding sequence
Gst/Halotag Coding Sequence, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gst+coding+sequence/luciferase+coding+sequence/pmc09820303-337-8-15
Average 90 stars, based on 1 article reviews
gst/halotag coding sequence - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Synthesized:

Article Title: Marburg Virus VP30 Is Required for Transcription Initiation at the Glycoprotein Gene
Article Snippet: .. The VP24/L NCR was synthesized by GenScript, and the firefly coding sequence was obtained by PCR from the pGL4.18[luc2P/Neo] vector (Promega). ..

Sequencing:

Article Title: Marburg Virus VP30 Is Required for Transcription Initiation at the Glycoprotein Gene
Article Snippet: .. The VP24/L NCR was synthesized by GenScript, and the firefly coding sequence was obtained by PCR from the pGL4.18[luc2P/Neo] vector (Promega). ..

Article Title: Efficient healing of large osseous segmental defects using optimized chemically modified messenger RNA encoding BMP-2.
Article Snippet: .. For the FLuc cmRNA, the FLuc coding sequence (amplified from pGL4.10 plasmid; Promega, USA) was used in combination with a minimal 5′UTR sequence. ..

Polymerase Chain Reaction:

Article Title: Marburg Virus VP30 Is Required for Transcription Initiation at the Glycoprotein Gene
Article Snippet: .. The VP24/L NCR was synthesized by GenScript, and the firefly coding sequence was obtained by PCR from the pGL4.18[luc2P/Neo] vector (Promega). ..

Luciferase:

Article Title: Oxaborole analogs and uses thereof
Article Snippet: The luciferase-expressing L. major parasite used in this assay is genetically modified by adding the luciferase coding region to the pLEXSY-hyg2 vector (Jena Biosciences). .. The luciferase expression construct was created by digesting the luciferase coding region (1.66 kbp) of pGL3-Basic (Promega) by using two restriction enzymes, NcoI/EagI, followed by electrophoretic separation of the luciferase coding region on a 1% agarose gel. .. The luciferase coding region was then ligated into pLEXSY-hyg2 vector (Jena Biosciences) which had previously been digested with NcoI/NotI.

Expressing:

Article Title: Oxaborole analogs and uses thereof
Article Snippet: The luciferase-expressing L. major parasite used in this assay is genetically modified by adding the luciferase coding region to the pLEXSY-hyg2 vector (Jena Biosciences). .. The luciferase expression construct was created by digesting the luciferase coding region (1.66 kbp) of pGL3-Basic (Promega) by using two restriction enzymes, NcoI/EagI, followed by electrophoretic separation of the luciferase coding region on a 1% agarose gel. .. The luciferase coding region was then ligated into pLEXSY-hyg2 vector (Jena Biosciences) which had previously been digested with NcoI/NotI.

Construct:

Article Title: Oxaborole analogs and uses thereof
Article Snippet: The luciferase-expressing L. major parasite used in this assay is genetically modified by adding the luciferase coding region to the pLEXSY-hyg2 vector (Jena Biosciences). .. The luciferase expression construct was created by digesting the luciferase coding region (1.66 kbp) of pGL3-Basic (Promega) by using two restriction enzymes, NcoI/EagI, followed by electrophoretic separation of the luciferase coding region on a 1% agarose gel. .. The luciferase coding region was then ligated into pLEXSY-hyg2 vector (Jena Biosciences) which had previously been digested with NcoI/NotI.

Agarose Gel Electrophoresis:

Article Title: Oxaborole analogs and uses thereof
Article Snippet: The luciferase-expressing L. major parasite used in this assay is genetically modified by adding the luciferase coding region to the pLEXSY-hyg2 vector (Jena Biosciences). .. The luciferase expression construct was created by digesting the luciferase coding region (1.66 kbp) of pGL3-Basic (Promega) by using two restriction enzymes, NcoI/EagI, followed by electrophoretic separation of the luciferase coding region on a 1% agarose gel. .. The luciferase coding region was then ligated into pLEXSY-hyg2 vector (Jena Biosciences) which had previously been digested with NcoI/NotI.

other:

Article Title: A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens
Article Snippet: A luciferase-coding sequence was amplified from pGL4.10 (Promega) and inserted into the Stu I site of pAK101, resulting in a gateway-luciferase binary vector pAK102.

Article Title: Chloroquine Potentiates the Anticancer Effect of Pterostilbene on Pancreatic Cancer by Inhibiting Autophagy and Downregulating the RAGE/STAT3 Pathway
Article Snippet: A luciferase-expressing MIA PaCa-2 cell line was established via transfection with a pGL-3 vector containing a luciferase-coding sequence (Promega, Madison, WI, USA), and selected by G418.

Article Title: Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway.
Article Snippet: Clathrin-mediated vesicle trafficking plays central roles in post-Golgi transport.. In yeast (Saccharomyces cerevisiae), the AP-1 complex and GGA adaptors are predicted to generate distinct transport vesicles at the trans-Golgi network (TGN), and the epsin-related proteins Ent3p and Ent5p (collectively Ent3p/5p) act as accessories for these adaptors.. Recently, we showed that vesicle transport from the TGN is crucial for yeast Rab5 (Vps21p)-mediated endosome formation, and that Ent3p/5p are crucial for this process, whereas AP-1 and GGA adaptors are dispensable.

Article Title: α N -Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers.
Article Snippet: The RT-PCR products were cloned downstream of the GST/HaloTag® coding sequence (Flexi® Vector, Promega, Madrid, Spain) and the TEV protease site, and when sequenced, the proteins were identical to the GenBankTM sequences.

Article Title: α N -Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers
Article Snippet: The RT-PCR products were cloned downstream of the GST/HaloTag ® coding sequence (Flexi ® Vector, Promega, Madrid, Spain) and the TEV protease site, and when sequenced, the proteins were identical to the GenBankTM sequences.

Amplification:

Article Title: Efficient healing of large osseous segmental defects using optimized chemically modified messenger RNA encoding BMP-2.
Article Snippet: .. For the FLuc cmRNA, the FLuc coding sequence (amplified from pGL4.10 plasmid; Promega, USA) was used in combination with a minimal 5′UTR sequence. ..

Plasmid Preparation:

Article Title: Efficient healing of large osseous segmental defects using optimized chemically modified messenger RNA encoding BMP-2.
Article Snippet: .. For the FLuc cmRNA, the FLuc coding sequence (amplified from pGL4.10 plasmid; Promega, USA) was used in combination with a minimal 5′UTR sequence. ..



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