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gsdmd antibody  (Novus Biologicals)


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    Novus Biologicals gsdmd antibody
    Figure 4: KORs inhibit <t>cell</t> <t>pyroptosis</t> through mediating cerebral pyrophosphorylation in CPB rats. (a) Expression levels of <t>GSDMD</t> and cleaved GSDMD-N-terminal in rats’ brain tissue samples were quantified with western blotting. (b) Counterstaining of GSDMD and TUNEL (scale bar 50 μm) was carried out for evaluation of pyrophoric and apoptotic rate, respectively. Relative pyroptotic and apoptotic rates were represented in the form of a bar chart. All data in charts were represented as mean ± standard deviation; “∗” was labeled when the comparative significance between groups (p value) was less than 0.05.
    Gsdmd Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gsdmd/pm34630980-110-0-3?v=Novus+Biologicals
    Average 94 stars, based on 10 article reviews
    gsdmd antibody - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k -Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway."

    Article Title: Reversing Postcardiopulmonary Bypass Associated Cognitive Dysfunction Using k -Opioid Receptor Agonists to Regulate Microglial Polarization via the NLRP3/Caspase-1 Pathway.

    Journal: Journal of healthcare engineering

    doi: 10.1155/2021/3048383

    Figure 4: KORs inhibit cell pyroptosis through mediating cerebral pyrophosphorylation in CPB rats. (a) Expression levels of GSDMD and cleaved GSDMD-N-terminal in rats’ brain tissue samples were quantified with western blotting. (b) Counterstaining of GSDMD and TUNEL (scale bar 50 μm) was carried out for evaluation of pyrophoric and apoptotic rate, respectively. Relative pyroptotic and apoptotic rates were represented in the form of a bar chart. All data in charts were represented as mean ± standard deviation; “∗” was labeled when the comparative significance between groups (p value) was less than 0.05.
    Figure Legend Snippet: Figure 4: KORs inhibit cell pyroptosis through mediating cerebral pyrophosphorylation in CPB rats. (a) Expression levels of GSDMD and cleaved GSDMD-N-terminal in rats’ brain tissue samples were quantified with western blotting. (b) Counterstaining of GSDMD and TUNEL (scale bar 50 μm) was carried out for evaluation of pyrophoric and apoptotic rate, respectively. Relative pyroptotic and apoptotic rates were represented in the form of a bar chart. All data in charts were represented as mean ± standard deviation; “∗” was labeled when the comparative significance between groups (p value) was less than 0.05.

    Techniques Used: Expressing, Western Blot, TUNEL Assay, Standard Deviation, Labeling



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    Image Search Results


    Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: Composite hydrogel promotes the polarization of BV2 cells to M2 types and alleviates PC12 cell pyroptosis in vitro inflammatory environment. (A) Representative western blots showing protein expression of iNOS and Arg-1 in each group, β-actin was utilized as a loading control. (B) Quantitative analysis of relative expression of iNOS and Arg-1. (C) Representative immunofluorescence images of CD68 positive and iNOS positive BV2 cells (scale bar: 20 μm). (D) Representative immunofluorescence images of CD68 positive and Arg-1 positive BV2 cells (scale bar: 20 μm). (E, F) Quantitative analysis of relative fluorescence intensity of iNOS and Arg-1. (G) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein associated with pyroptosis, β-actin was utilized as a loading control. (H) PI staining of PC12 cells in each group (scale bar, 100 μm). (I) Quantitative analysis of PI staining of PC12 cells (n = 3). (J) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: Antibodies against GSDMD-N (cat. no. DF12275), CD44 (cat. no. BF9213), and Integrin beta1 (cat. no. AF5379) were obtained from Affinity Biosciences in Ohio, USA.

    Techniques: In Vitro, Western Blot, Expressing, Control, Immunofluorescence, Fluorescence, Staining, Comparison

    The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Journal: Bioactive Materials

    Article Title: 3D-MSCs apoptotic bodies-integrated conductive hydrogel mitigates spinal cord injury via immunoregulation and alleviating neuronal pyroptosis

    doi: 10.1016/j.bioactmat.2026.01.043

    Figure Lengend Snippet: The composite hydrogel inhibits post-SCI pyroptosis in neurons. (A) Immunofluorescence images of residual neurons existing in the anterior horn of the spinal cord (scale bar, 500 μm and 200 μm). (B) Immunofluorescence image of Caspase-1 expression of neurons 3 days after SCI (scale bar, 20 μm). (C) Quantitative analysis of relative fluorescence intensity of Caspase-1 protein expression in neurons within the specified groups (n = 3). (D) Immunofluorescence image of GSDMD-N expression of neurons 3 days after SCI (scale bar, 20 μm). (E) Quantitative analysis of relative fluorescence intensity of GSDMD-N protein expression in neurons within the specified groups (n = 3). (F) Representative western blots showing the expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N, and IL-18 protein 3 days after SCI, GAPDH was utilized as a loading control. (G) Quantitative analysis of relative expression of NLRP3, Caspase-1, IL-1β, ASC, GSDMD-N and IL-18 (n = 3). The data are presented as the means ± SEMs (n = 3); ∗p < 0.05, indicates significant differences; ns, is not significant. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test.

    Article Snippet: Antibodies against GSDMD-N (cat. no. DF12275), CD44 (cat. no. BF9213), and Integrin beta1 (cat. no. AF5379) were obtained from Affinity Biosciences in Ohio, USA.

    Techniques: Immunofluorescence, Expressing, Fluorescence, Western Blot, Control, Comparison