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Synthego Inc grna sequence 2

Grna Sequence 2, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/grna-2+sequence/grna+sequences/pmc10694670-100-0-5
Average 90 stars, based on 1 article reviews
grna sequence 2 - by Bioz Stars, 2026-09
90/100 stars

Images

1) Product Images from "TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma"

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

Journal: iScience

doi: 10.1016/j.isci.2023.108353


Figure Legend Snippet:

Techniques Used: Recombinant, Lactate Dehydrogenase Assay, Cell Based Assay, Gene Knockout, Enzyme-linked Immunosorbent Assay, shRNA, Sequencing, Software, Gene Expression

Related Articles

Sequencing:

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson’s disease–linked DJ-1
Article Snippet: .. The gRNA target sequence (5′-TAA GGT CAC CGT TGC AGG CC-3′) for DJ-1 exon three was designed using SYNTHEGO ( https://design.synthego.com/#/ ). ..

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma
Article Snippet: gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A. .. gRNA sequence 3: GGCCAUUUGUAAUGCUGACU , Synthego , N/A. ..

Article Title: The reaction mechanism for glycolysis side product degradation by Parkinson's disease-linked DJ-1.
Article Snippet: .. The gRNA target sequence (59-TAAGGT CACCGTTGCAGGCC-39) for DJ-1 exon three was designed using Watanabe et al. Journal of Cell Biology 16 of 21 Reactive lactone degradation by DJ-1/PARK7 https://doi.org/10.1083/jcb.202411078 D ow nloaded from http://rupress.org/jcb/article-pdf/224/8/e202411078/1945774/jcb_202411078.pdf by Viet N am user on 07 June 2025 SYNTHEGO (https://design.synthego.com/#/). ..

Article Title: AAA+ ATPase chaperone p97/VCP FAF2 governs basal pexophagy
Article Snippet: .. To establish FIP200 knockout HCT116 cells and PEX19/FIP200, FAF2/FIP200 double knockout HCT116 cells, the gRNA target sequence (5’- CAAGATTGCTATTCAACACC-3’) was designed using online CRISPR design tool (SYNTHEGO) to a region in exon 4 of FIP200. ..

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma
Article Snippet: gRNA sequence 1: UCUUCCCUAGGAAUGAUGAC , Synthego , N/A. .. gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A. .. gRNA sequence 3: GGCCAUUUGUAAUGCUGACU , Synthego , N/A.

Retroviral:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Transfection:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Transduction:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Construct:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Recombinant:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Plasmid Preparation:

Article Title: The transcription regulator ID3 maintains tumor-specific memory CD8 + T cells in draining lymph nodes during tumorigenesis.
Article Snippet: .. Retroviral construction, transfection and transduction To construct the pSL21-VEX-Id3-gRNA retroviral recombinant plasmid, four gRNA sequences (refer to the Table S1) targeting the mouse Id3 gene were initially designed using Synthego (https://www.synthego.com). .. Oligo fragments of the corresponding gRNA sequence (refer to the Table S1) and its reverse sequence were synthesized at Invitrogen and then linked to the pSL21-VEX reverse vector.

Generated:

Article Title: Genomic studies controvert the existence of the CUX1 p75 isoform
Article Snippet: Nitrocellulose membrane (Thermo Scientific, Catalog number: 88018) was used for protein transfer and ECL substrate (Thermo Scientific, Catalog number: 34579) was used for visualizing the proteins of interest. .. All gRNAs were designed using the Broad Institute’s sgRNA designer tool, and generated gRNA sequences were verified using Synthego’s Verify Guide Design tool. gRNA sequences used in this study were purchased from Synthego, and the sequences are listed below: CUX1 exon 4 gRNA: 5′-UGCACUGAGUAAAAGAAGCA-3′. ..

Knock-Out:

Article Title: AAA+ ATPase chaperone p97/VCP FAF2 governs basal pexophagy
Article Snippet: .. To establish FIP200 knockout HCT116 cells and PEX19/FIP200, FAF2/FIP200 double knockout HCT116 cells, the gRNA target sequence (5’- CAAGATTGCTATTCAACACC-3’) was designed using online CRISPR design tool (SYNTHEGO) to a region in exon 4 of FIP200. ..

Double Knockout:

Article Title: AAA+ ATPase chaperone p97/VCP FAF2 governs basal pexophagy
Article Snippet: .. To establish FIP200 knockout HCT116 cells and PEX19/FIP200, FAF2/FIP200 double knockout HCT116 cells, the gRNA target sequence (5’- CAAGATTGCTATTCAACACC-3’) was designed using online CRISPR design tool (SYNTHEGO) to a region in exon 4 of FIP200. ..

CRISPR:

Article Title: AAA+ ATPase chaperone p97/VCP FAF2 governs basal pexophagy
Article Snippet: .. To establish FIP200 knockout HCT116 cells and PEX19/FIP200, FAF2/FIP200 double knockout HCT116 cells, the gRNA target sequence (5’- CAAGATTGCTATTCAACACC-3’) was designed using online CRISPR design tool (SYNTHEGO) to a region in exon 4 of FIP200. ..



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Image Search Results


Journal: iScience

Article Title: TIGIT contributes to the regulation of 4-1BB and does not define NK cell dysfunction in glioblastoma

doi: 10.1016/j.isci.2023.108353

Figure Lengend Snippet:

Article Snippet: gRNA sequence 2: AUGUCACCUCUCCUCCACCA , Synthego , N/A.

Techniques: Recombinant, Lactate Dehydrogenase Assay, Cell Based Assay, Gene Knockout, Enzyme-linked Immunosorbent Assay, shRNA, Sequencing, Software, Gene Expression

Plasmids used in this work

Journal: Microbial Cell Factories

Article Title: Development of an industrial yeast strain for efficient production of 2,3-butanediol

doi: 10.1186/s12934-022-01924-z

Figure Lengend Snippet: Plasmids used in this work

Article Snippet: P58-PDC1 , P58 backbone with 2 gRNA targeting sequence for PDC1 (AGCATCCAACAATTTTTGCA and GATAAGCTTTATGAAGTCAA) , MCB, KU Leuven.

Techniques: Marker, Plasmid Preparation, Sequencing, Expressing