gradient master (Biocomp Instruments Inc)

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Biocomp Instruments Inc gradient master
Gradient Master, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient+system+master/gradient+master/pmc13065467-274-22-24
Average 86 stars, based on 1 article reviews

gradient master - by Bioz Stars, 2026-11

86/100 stars

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Article Title: Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex
Article Snippet: Two buffers were used to prepare 10–30% continuous glycerol gradients (4 ml total volume) with an additional 0.0–0.2% glutaraldehyde component using a gradient master (Biocomp Instruments): buffer A contained 20 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM TCEP, and buffer B contained 20 mM HEPES, pH 7.5, 150 mM NaCl, 30% glycerol, 1 mM TCEP, 0.2% glutaraldehyde.

Article Title: Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.
Article Snippet: Gradients were made with a gradient maker (BioComp Instruments) and left to equilibrate at 4 °C for at least 1 hour.

Article Title: Decoupling nucleolar stress from DNA-damage in HCT116 colon cancer cells by targeting the interaction between ribosomal assembly factors Bop1/WDR12
Article Snippet: The 10%– 50% sucrose linear gradients were prepared in the Gradient Master (Biocomp Instruments) and kept cold until use.

Article Title: Mechanisms of CARMIL dimerization, autoinhibition, and capping protein binding
Article Snippet: They were then loaded onto 5 to 30% glycerol gradients prepared using a Gradient Master (BioComp Instruments) and centrifuged at 40,000 rpm for ~16 hours at 4°C using a Beckman SW 60 Ti rotor.

Article Title: Mechanisms of disassembly at the actin filament pointed and barbed ends
Article Snippet: The complex, in 20 mM Hepes (pH 7.5) and 100 mM NaCl, was loaded onto a 5 to 30% glycerol gradient prepared with a Gradient Master (BioComp Instruments), centrifuged at 40,000 rpm for 16 hours at 4°C using a Beckman SW 60 Ti rotor, and fractionated with a Piston Gradient Fractionator (BioComp Instruments).

Article Title: Mechanisms of CARMIL dimerization, autoinhibition, and capping protein binding.
Article Snippet: They were then loaded onto 5 to 30% glycerol gradients prepared using a Gradient Master (BioComp Instruments) and centrifuged at 40,000 rpm for ~16 hours at 4°C using a Beckman SW 60 Ti rotor.

Article Title: Mechanisms of disassembly at the actin filament pointed and barbed ends.
Article Snippet: The complex, in 20 mM Hepes (pH 7.5) and 100 mM NaCl, was loaded onto a 5 to 30% glycerol gradient prepared with a Gradient Master (BioComp Instruments), centrifuged at 40,000 rpm for 16 hours at 4°C using a Beckman SW 60 Ti rotor, and fractionated with a Piston Gradient Fractionator (BioComp Instruments).

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Article Title: Histone surface exposure drives nucleosome stacking diversity through DNA engagement in trans.
Article Snippet: Intrinsic nucleosome dynamics, particularly changes in entry–exit DNA wrapping due to nucleosome breathing, contribute to local chromatin structural heterogeneity.. This process exposes otherwise buried histone surfaces, yet the structural mechanisms by which these changes drive diverse nucleosome stacking arrangements remain poorly understood.. Here, we determined cryo-EM structures of nucleosome core particles (NCPs) reconstituted with DNA truncated at superhelical locations (SHL) –4.5 and – 5.5, mimicking varying extents of histone surface exposure at the nucleosome entry– exit site.

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