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Cosmo Bio USA gradient master
Gradient Master, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient+master+gradient+maker/gradient+master/pmc07042231-281-73-75
Average 90 stars, based on 1 article reviews
gradient master - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Suspension:

Article Title: Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells
Article Snippet: .. To prepare crude lysate, the cell suspension was incubated on ice for 10 min, and the insoluble fraction was removed by centrifugation at 7,300 g, 4 °C for 15 min. Sucrose gradients (10–50% sucrose in 20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM DTT, 100 μg/mL CHX) were prepared in 14 × 89 mm open-top polyclear centrifuge tubes (#7030, Seton Scientific, Petaluma, CA, USA) using a Gradient Master (Cosmo Bio, Koto, Tokyo, Japan). .. The lysates (equivalent of 50 A260 units) were placed on top of the gradients and then centrifuged at 280,000 g in a SW41Ti rotor (Beckman Coulter, Brea, CA, USA) for 1.5 h at 4 °C.

Incubation:

Article Title: Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells
Article Snippet: .. To prepare crude lysate, the cell suspension was incubated on ice for 10 min, and the insoluble fraction was removed by centrifugation at 7,300 g, 4 °C for 15 min. Sucrose gradients (10–50% sucrose in 20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM DTT, 100 μg/mL CHX) were prepared in 14 × 89 mm open-top polyclear centrifuge tubes (#7030, Seton Scientific, Petaluma, CA, USA) using a Gradient Master (Cosmo Bio, Koto, Tokyo, Japan). .. The lysates (equivalent of 50 A260 units) were placed on top of the gradients and then centrifuged at 280,000 g in a SW41Ti rotor (Beckman Coulter, Brea, CA, USA) for 1.5 h at 4 °C.

Centrifugation:

Article Title: Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells
Article Snippet: .. To prepare crude lysate, the cell suspension was incubated on ice for 10 min, and the insoluble fraction was removed by centrifugation at 7,300 g, 4 °C for 15 min. Sucrose gradients (10–50% sucrose in 20 mM HEPES-KOH pH 7.4, 100 mM KCl, 5 mM MgCl 2 , 1 mM DTT, 100 μg/mL CHX) were prepared in 14 × 89 mm open-top polyclear centrifuge tubes (#7030, Seton Scientific, Petaluma, CA, USA) using a Gradient Master (Cosmo Bio, Koto, Tokyo, Japan). .. The lysates (equivalent of 50 A260 units) were placed on top of the gradients and then centrifuged at 280,000 g in a SW41Ti rotor (Beckman Coulter, Brea, CA, USA) for 1.5 h at 4 °C.



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