amaxa human cd34 cell nucleofector kit (Lonza)
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amaxa human cd34 cell nucleofector kit
Amaxa Human Cd34 Cell Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+rt+rt-pcr+kit/4d+nucleofector/pmc04196775-155-19-24
Average 90 stars, based on 1 article reviews
Amaxa Human Cd34 Cell Nucleofector Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+rt+rt-pcr+kit/4d+nucleofector/pmc04196775-155-19-24
Average 90 stars, based on 1 article reviews
amaxa human cd34 cell nucleofector kit - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "Integrated mRNA-MicroRNA Profiling of Human NK Cell Differentiation Identifies MiR-583 as a Negative Regulator of IL2Rγ Expression"
Article Title: Integrated mRNA-MicroRNA Profiling of Human NK Cell Differentiation Identifies MiR-583 as a Negative Regulator of IL2Rγ Expression
Journal: PLoS ONE
doi: 10.1371/journal.pone.0108913
Figure Legend Snippet: (a) Isolated HPC (CD34 + Lin − ) cells were cultured in IL-15-supplemented media, and the culture media was replaced every 48 h. The expression of CD56 as an NK cell surface marker was analyzed using FACS. (b) The kinetics of the mRNA expression of Id2, NKp30, NKG2D and Granzyme B were analyzed by real-time qPCR. (c) Stage 2 progenitors (CD34 + CD117 + CD94 − ) and stage 3 progenitors (CD34 − CD117 + CD94 − ) were isolated from UCB by flow sorting. The mRNA expression of IL2Rγ, NKp46 and NKG2D were analyzed by real-time qPCR. (d) A dendrogram of hierarchical clustering revealed genes that were altered more than 2-fold in 7d- and 14-d (mNK) cultured NK cell compared with 1 d-cultured cells. (E-F) The bar graphs represent the top seven functional categories of upregulated (e) or downregulated (f) genes according to the gene ontology analysis (as determined by DAVID, as described in the Methods). The data are representative of three independent experiments performed using three different UCB samples and represent the mean values ± S.E.M. of duplicates.
Techniques Used: Isolation, Cell Culture, Expressing, Marker, Functional Assay
Figure Legend Snippet: (a) Negatively correlated miRNA-mRNA interactions were visualized as a network using Magia (miRNAs and genes integrated analysis web-based tool). This network provides for the first time a theoretical outline of the concerted action of regulating miRNAs (red triangles) and their potential target mRNAs (green circles). (b) Isolated HPC (CD34 + Lin − ) cells were cultured as described in the Materials and Methods. After being cultured in IL-15-supplemented media, the cells were collected at the indicated time intervals. The expression of miR-143, miR-223, miR-150 and miR-583 was analyzed by real-time qPCR. The data are representative of five independent experiments performed using two different UCB samples and represent the mean values ± S.E.M. of duplicates.
Techniques Used: Isolation, Cell Culture, Expressing
Figure Legend Snippet: Isolated CB HPCs (CD34 + Lin − cells) were cultured as described in the Materials and Methods. After being cultured in IL-15-supplemented media, the cells were collected at 48 h intervals. (a) The expression of the IL2Rγ gene was analyzed by real-time quantitative RT-PCR. (b) The expression of IL2Rγ protein was analyzed by FACS. (c) Expression profiling of IL2Rγ, miR-583 and miR-143 after IL-15 treatment. The data are representative of three independent experiments performed using five different UCB samples and represent the mean values ± S.E.M. of duplicates.
Techniques Used: Isolation, Cell Culture, Expressing, Quantitative RT-PCR
Figure Legend Snippet: (a) Differentiating cells were transfected with miR-143, a miR-583 mimic or negative control miRNA. NK cell populations (CD56 + CD3 − cells) were analyzed by FACS 10 days after transfection. *, p <0.1. (b) The expression level of miR-583 was decreased during NK cell development as shown by qRT-PCR. The results are shown as the mean expression values normalized against stage 2 (CD34 + CD117 + CD94 − ). *, p <0.1. (c) NK cell populations (CD56 + CD3 − cells) were analyzed by FACS after miR-583 transfection at regular intervals during NK cell differentiation. The absolute numbers of differentiated NK cells are shown in the parentheses (×10 5 ). *, p <0.01 **, p <0.001. The data are representative of three independent experiments performed using three different UCB samples and represent the mean values ± S.E.M. of duplicates.
Techniques Used: Transfection, Negative Control, Expressing, Quantitative RT-PCR, Cell Differentiation
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