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caspase1 activity assay kit  (Beyotime)


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    Beyotime caspase1 activity assay kit
    Fig. 1. EV-A71 infection triggers cell death of SH-SY5Y cells. (A) The percentage of cells undergoing cell death was calculated through Annexin V-FITC/PI double staining. (B) The cell viability was conducted with Cell Counting Kit-8. (C) Levels of LDH released into the cell culture medium from membrane pores were examined using an LDH Cytotoxicity Assay Kit. (D) <t>Caspase1</t> activity was assessed with a Caspase1 Activity Assay Kit. .
    Caspase1 Activity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+rt+rt-pcr+kit/Caspase+1+Activity+Assay+Kit/pm37579846-86-10-14
    Average 96 stars, based on 546 article reviews
    caspase1 activity assay kit - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis."

    Article Title: The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis.

    Journal: Virus research

    doi: 10.1016/j.virusres.2023.199195

    Fig. 1. EV-A71 infection triggers cell death of SH-SY5Y cells. (A) The percentage of cells undergoing cell death was calculated through Annexin V-FITC/PI double staining. (B) The cell viability was conducted with Cell Counting Kit-8. (C) Levels of LDH released into the cell culture medium from membrane pores were examined using an LDH Cytotoxicity Assay Kit. (D) Caspase1 activity was assessed with a Caspase1 Activity Assay Kit. .
    Figure Legend Snippet: Fig. 1. EV-A71 infection triggers cell death of SH-SY5Y cells. (A) The percentage of cells undergoing cell death was calculated through Annexin V-FITC/PI double staining. (B) The cell viability was conducted with Cell Counting Kit-8. (C) Levels of LDH released into the cell culture medium from membrane pores were examined using an LDH Cytotoxicity Assay Kit. (D) Caspase1 activity was assessed with a Caspase1 Activity Assay Kit. .

    Techniques Used: Infection, Double Staining, Cell Counting, Cell Culture, Membrane, LDH Cytotoxicity Assay, Activity Assay

    Fig. 2. EV-A71 infection induces NLRP3-dependent pyroptosis in SH-SY5Y cells. (A) NLRP3, ASC, Casepase1, Gasdermin D, IL-1β and IL-18 mRNA levels were analyzed by qRT-PCR. (B) The abundance of NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 was determined by WB analysis. (C) At 48 and 72 h post infection, infected SH-SY5Y cells were subjected to indirect immunofluorescence assays with anti-VP1 (red) and anti-Caspase-1 (green) antibodies. (D) Secretion of IL-1β and IL-18 upon infection with EV-A71 was measured with a commercial ELISA kit. .
    Figure Legend Snippet: Fig. 2. EV-A71 infection induces NLRP3-dependent pyroptosis in SH-SY5Y cells. (A) NLRP3, ASC, Casepase1, Gasdermin D, IL-1β and IL-18 mRNA levels were analyzed by qRT-PCR. (B) The abundance of NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 was determined by WB analysis. (C) At 48 and 72 h post infection, infected SH-SY5Y cells were subjected to indirect immunofluorescence assays with anti-VP1 (red) and anti-Caspase-1 (green) antibodies. (D) Secretion of IL-1β and IL-18 upon infection with EV-A71 was measured with a commercial ELISA kit. .

    Techniques Used: Infection, Quantitative RT-PCR, Immunofluorescence, Enzyme-linked Immunosorbent Assay

    Fig. 8. has_circ_0045431/has-miR-584/NLRP3 regulatory axis promotes EV-A71-induced pyroptosis formation. (A) SH-SY5Y cells with different treatments were subjected to WB analysis for NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 expression. (B) The cell culture supernatant was subjected to the examination of ELISA for IL-1β and IL-18 concentration.
    Figure Legend Snippet: Fig. 8. has_circ_0045431/has-miR-584/NLRP3 regulatory axis promotes EV-A71-induced pyroptosis formation. (A) SH-SY5Y cells with different treatments were subjected to WB analysis for NLRP3, ASC, pro-Caspase1, cleaved Caspase 1, Gasdermin D, IL-1β and IL-18 expression. (B) The cell culture supernatant was subjected to the examination of ELISA for IL-1β and IL-18 concentration.

    Techniques Used: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Related Articles

    Activity Assay:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Article Title: Ningxintong and its active component calycosin stabilize vulnerable atherosclerotic plaques by targeting SIRT7 and inhibiting pyroptosis.
    Article Snippet: Objective: To investigate the regulatory effect of Ningxintong (NXT) on monocyte/macrophage pyroptosis in atherosclerosis (AS), elucidate its molecular mechanisms, and identify its key active components.. Methods: The expression of inflammationand pyroptosis-related molecules was detected in peripheral blood monocytes from patients with AS.. An atherosclerotic vulnerable plaque (AS-VP) mouse model was established to evaluate the effects of NXT on blood lipid levels, plaque formation, and stability.

    Article Title: High‐Throughput Screening Reveals That CeeNU Acts as a New NLRP3 Inflammasome Inhibitor
    Article Snippet: Anti‐mouse IL‐1β, Human IL‐1β, IL‐6, and TNF‐α ELISA kits (CEK1788, CEK1731, CEK1785, CEK1783) were from Bioworld (USA). .. Cytotoxicity (LDH) assay kit ( C10007 ), Caspase‐1 activity assay kit (C1102), and Flagellin (P7388) were from Beyotime (Shanghai, China). .. Anti‐CD4‐APC (317416, 1:400) and anti‐CD45‐PE‐Cyanine7 (25‐0451‐82, 1:400) antibodies were from Biolegend (San Diego, CA, USA).

    Article Title: DS-10 mitigates cerebral ischemia-reperfusion injury in mice through suppressing caspase-1-mediated pyroptosis.
    Article Snippet: Ischemic stroke (IS) is an acute cerebrovascular event with substantial mortality and morbidity, yet effective therapies are lacking.. This study investigates the protective properties of DS-10, a decapeptide from Dunaliella salina, against cerebral ischemia-reperfusion injury (CIRI).. Using a mouse model of middle cerebral artery occlusion/reperfusion (MCAO/R) and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated BV2 microglial cells, we evaluated the effects of DS-10.

    Staining:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Immunostaining:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Blocking Assay:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    CCK-8 Assay:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Lysis:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Western Blot:

    Article Title: Infectious bronchitis virus accessory protein 3a induces renal inflammatory injury by activating the NLRP3 inflammasome via ER calcium mobilization and mitochondrial ROS production.
    Article Snippet: Lipofectamine 2000 Transfection Reagent (11668019) was purchased from Invitrogen. .. Caspase 1 Activity Assay Kit, Immunol Staining Fix Solution, Immunostaining Permeabilization Buffer containing Triton X-100, Immunol Staining Blocking Buffer, CCK-8 solution, Cell Lysis Buffer for Western and IP, Mito-Tracker Green, ER-Tracker Red, Mito-ROS, and Hoechst were purchased from Beyotime, China. ..

    Lactate Dehydrogenase Assay:

    Article Title: High‐Throughput Screening Reveals That CeeNU Acts as a New NLRP3 Inflammasome Inhibitor
    Article Snippet: Anti‐mouse IL‐1β, Human IL‐1β, IL‐6, and TNF‐α ELISA kits (CEK1788, CEK1731, CEK1785, CEK1783) were from Bioworld (USA). .. Cytotoxicity (LDH) assay kit ( C10007 ), Caspase‐1 activity assay kit (C1102), and Flagellin (P7388) were from Beyotime (Shanghai, China). .. Anti‐CD4‐APC (317416, 1:400) and anti‐CD45‐PE‐Cyanine7 (25‐0451‐82, 1:400) antibodies were from Biolegend (San Diego, CA, USA).



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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    Image Search Results


    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Journal: Nature microbiology

    Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

    doi: 10.1038/s41564-017-0028-z

    Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

    Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation