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bca protein estimation kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bca protein estimation kit
    Bca Protein Estimation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+rt+rt-pcr+kit/BCA+Protein+Assay+Kit/pm32635858-39-10-52
    Average 99 stars, based on 1 article reviews
    bca protein estimation kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    BIA-KA:

    Article Title: Screening of a quinonoid compounds library identifies decylubiquinone as an antioxidant and anti-apoptotic agent against glucocorticoid-induced osteoporosis via CD39/CD73/adenosine axis
    Article Snippet: Nuclear and cytoplasmic proteins were separated using Cytoplasm Abstraction Reagent and NE-PER Nuclear Extraction Reagents (P0028, Beyotime, Shanghai, China). .. Protein concentrations were determined using a BCA assay kit (23225, Thermo, Rockford, IL, USA). .. 20 μg total protein of each sample was separated by SDS-PAGE and transferred to PVDF membranes (ISEQ00010, Millipore Sigma, MA, USA).

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The protein concentration was determined by Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). ..

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation
    Article Snippet: For the quantitative measurement of ALP activity, the Alkaline Phosphatase Assay Kit (P0321S, Beyotime, China) was used according to the manufacturer's instructions. .. The total protein content of each sample was quantified with a Micro BCA Protein Assay Kit (Thermo Fisher Scientific, USA). .. After 21 days of co-culture with osteogenic extracts, mineralized calcium nodules in MC3T3-E1 cells were stained using Alizarin Red S Solution (OriCell, China).

    Article Title: A bioactive magnesium alloy scaffold integrated with BMSCs-Loaded 3D microspheres synergistically promotes femoral head osteonecrosis repair by improving the osteogenic-angiogenic microenvironment
    Article Snippet: .. Protein concentrations were determined using a BCA assay kit (Thermo Fisher Scientific, USA). .. Equal amounts of total protein (30 μg per sample) were separated by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA).

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle
    Article Snippet: .. Protein content of the supernatants was determined by BCA Protein Assay Kit (Pierce Biotechnology, Rockford, IL, USA). ..

    Protein Concentration:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The protein concentration was determined by Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). ..

    Article Title: Title Pending 17760
    Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05).

    Article Title: Targeting VEGFR2 inhibition within a spatially-confined conduit promotes nerve self-resolution and alleviates mechanical allodynia
    Article Snippet: Total protein was extracted from the rat DRG cell line FH-R182 (Fuheng Biology, China) and from HUVECs (NICR, catalog number 4201PAT-CCTCC02060, Beijing, China) using Cell Lysis Buffer for Western Blot and IP (Beyotime, P0013J). .. Protein concentration was measured with a BCA Protein Assay Kit (Thermo Fisher Scientific, 23227). .. Equal amounts of protein were separated on 7.5% or 15% SDS–PAGE gels and transferred onto PVDF membranes (Millipore, IPVH00010).

    Bicinchoninic Acid Protein Assay:

    Article Title: Title Pending 17760
    Article Snippet: This study investigated the effect of freezing and subsequent aging on beef quality, particularly focusing on the extent of postmortem proteolysis and tenderization.. The longissimus lumborum muscle was collected from 8 steers 24 h postmortem, sliced into 8 2.5-cm-thick steaks, and randomly allocated into 4 groups.. Treatment groups consisted of 1) aging at 4°C for 24 h; 2) aging for 168 h; 3) freezing at −20°C for 24 h followed by thawing/aging for 24 h; and 4) freezing for 24 h followed by thawing/aging for 168 h. In general, freezing decreased the color intensity of the steaks, whereas aging increased it (P< 0.05).

    Article Title: Targeting VEGFR2 inhibition within a spatially-confined conduit promotes nerve self-resolution and alleviates mechanical allodynia
    Article Snippet: Total protein was extracted from the rat DRG cell line FH-R182 (Fuheng Biology, China) and from HUVECs (NICR, catalog number 4201PAT-CCTCC02060, Beijing, China) using Cell Lysis Buffer for Western Blot and IP (Beyotime, P0013J). .. Protein concentration was measured with a BCA Protein Assay Kit (Thermo Fisher Scientific, 23227). .. Equal amounts of protein were separated on 7.5% or 15% SDS–PAGE gels and transferred onto PVDF membranes (Millipore, IPVH00010).



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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) <t>PCR</t> demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , <t>RT-PCR</t> of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.
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    a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Journal: Nature microbiology

    Article Title: A metabolic pathway for catabolizing levulinic acid in bacteria

    doi: 10.1038/s41564-017-0028-z

    Figure Lengend Snippet: a , Organization of the lvaRABCDEFG (9,323 bp) operon. b , Reverse Transcriptase (RT) PCR demonstrates that each gene is expressed in cells grown on LA. Samples were compared with the negative control (-RT) where reverse transcriptase was omitted from the reaction ( n =1). c , RT-PCR of cDNA created with primer JMR237 demonstrates that the operon is polycistronic. Note that a product spanning each intergenic region was observed ( n =1). d , lva operon induction assay. GFP fluorescence was measured from LB-cultures supplemented with various organic acids (20 mM) ( n =3, biological). Error bars represent s.d. Insert shows the schematic of transcriptional GFP fusion used to test induction of the lva operon. lvaR was cloned onto a plasmid containing its native constitutive promoter and the native promoter region for lvaA . The fluorescent protein sfGFP was cloned in place of lvaA . e , Proposed pathway for LA metabolism. LA, levulinic acid; 4HV, 4-hydroxyvalerate; 3HV, 3-hydroxyvalerate; LA-CoA, levulinyl-CoA; 4HV-CoA, 4-hydroxyvaleryl-CoA; CoA, coenzyme-A; ATP, adenosine triphosphate; 4PV-CoA, 4-phosphovaleryl-CoA; 3KV-CoA, 3-ketovaleryl-CoA; NAD(P)H, Nicotinamide adenine dinucleotide (phosphate) reduced; GFP, green fluorescent protein.

    Article Snippet: The Promega GoScript RT PCR kit was used to generate cDNA using 1 μL of a 10 μM gene specific oligo (JMR2 for lvaR and JMR287 for lvaA ) instead of the random oligo mixture.

    Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Negative Control, Fluorescence, Clone Assay, Plasmid Preparation