iscript reverse transcriptase kit (Bio-Rad)
Bio-Rad is a verified supplier 
99
Structured Review
Bio-Rad
iscript reverse transcriptase kit
Iscript Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+reverse+transcriptase+rt-pcr+kit/iScript+cDNA+Synthesis+Kit/pmc08544493-38-3-19
Average 99 stars, based on 49575 article reviews
Iscript Reverse Transcriptase Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goscript+reverse+transcriptase+rt-pcr+kit/iScript+cDNA+Synthesis+Kit/pmc08544493-38-3-19
Average 99 stars, based on 49575 article reviews
iscript reverse transcriptase kit - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "Improved SARS-CoV-2 PCR detection and genotyping with double-bubble primers"
Article Title: Improved SARS-CoV-2 PCR detection and genotyping with double-bubble primers
Journal: Biotechniques
doi: 10.2144/btn-2021-0063
Figure Legend Snippet: (A–C) Dose–response curves using double-bubble primer mix #8 and VIC-TqM probe #12 and decimal dilutions of SARS-CoV-2 synthetic RNA N. (A) Real-time amplification plot. C t values: 8 × 10 5 copies, 22.82; 8 × 10 4 copies, 26.27; 8 × 10 3 copies, 30.55; 8 × 10 2 copies, 32.51. (B) 5% agarose gel of the PCR amplification products. Lane 1: ultra-low-range ladder; lanes 2–5: 139-bp amplicons of 8 × 10 5 to 8 × 10 2 RNA copies per tube. Also included in the PCR reaction mix were 2× TqM buffer without UDP and iScript reverse-transcriptase. (C) Efficiency plot of the PCR reaction depicted in panel A. Slope = -3.3359 represents PCR reaction efficiency of 99.42%; R 2 = 0.9816. (D) One-tube RT-qPCR using double-bubble primer mix #10 from region 2 using SARS-CoV-2 synthetic RNA (2 × 10 4 copies per tube) and FAM-TqM probe #13. RT-qPCR was performed with standard conditions.
Techniques Used: Amplification, Agarose Gel Electrophoresis, Reverse Transcription, Quantitative RT-PCR
Figure Legend Snippet: (A) qPCR using reverse-transcribed cDNA template and double-bubble (D-B) primer mix #10, FAM-TqM probe #13, TqM fast kit+ uracil-DNA glycosylase (UDG) assayed in duplicates. Insert depicts 5% agarose gel; lane 1: ultra-low-range (ULR) ladder; lanes 2 & 3: template cDNA; lanes 4 & 5: non-template control (NTC). C t average values: cDNA, 28.60; NTC, undetermined. (B) One-tube RT-qPCR using SARS-CoV-2 synthetic RNA N template and D-B primer mix #8, VIC-TqM probe #12, TqM fast kit without UDG iScript reverse transcriptase, assayed in duplicate. Insert depicts 5% agarose gel; lane 1: ULR ladder; lanes 2 and 3: template RNA; lanes 4 and 5: NTC. C t average values: RNA, 24.63, NTC, undetermined. (C) Duplex qPCR using SARS-CoV-2 synthetic RNA N template and TqM fast kit with UDG. C t values: primer 8, 26.13; primer 10, 32.19; duplex primers 8 + 10, 26.36 and 30.27, respectively; NTC, undetermined. (D) 5% agarose gel of samples shown in panel C. Lane 1: ULR ladder; lane 2: primer D-B mix #8 (amplicon 139 bp); lane 3: D-B primer mix #10 (amplicon 158 bp); lane 4: duplex of both primers #8 and #10 (amplicons 139 and 158 bp). Arrows (C & D) show duplex amplifications in the same tube with D-B primers #8 and #10. PCR was performed using fast conditions with 40 (A, C & D) and 30 (B) cycles.
Techniques Used: Reverse Transcription, Agarose Gel Electrophoresis, Control, Quantitative RT-PCR, Amplification
Figure Legend Snippet: The reaction mixture contained hot-start double-bubble (D-B) primer mix #4, VIC-TqM probe #12, cost-effective non-hot-start Taq polymerase (FroggaBio), reverse transcriptase (iScript), synthetic SARS-CoV-2 RNA gene N as template, added dNTPs and ROX dye for internal calibration. The reaction was assembled at room temperature and subjected to real time RT-qPCR using the following fast conditions: 42°C, 15 min; 95°C,1 min; 30 cycles of 95°C, 1 s → 70°C, 20 s. The amplification plot and (insert) 5% agarose gel depict the amplification of SARS-CoV-2 RNA extracted from the nasopharyngeal swabs of patient S1 (lane 2) and negative patient N1 (lane 3), no-template controls (lanes 4 and 5) and SARS-CoV-2 gene N synthetic RNA as positive control (lane 6). Note the amplification in the real-time plot and the 115-bp band in the 5% agarose gel with SARS-CoV-2 virus or synthetic RNA (lanes 2 and 6), with no amplifications in the negative control N1 (lane 3) or NTC (lanes 4 and 5). Lane 1: ultra-low-range ladder. C t values: positive patient S1: 23.95; synthetic RNA: 23.84; negative patient N1 and NTC: undetermined.
Techniques Used: Reverse Transcription, Quantitative RT-PCR, Amplification, Agarose Gel Electrophoresis, Positive Control, Virus, Negative Control
Related Articles
Reverse Transcription:Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC Article Snippet: The RNA purity was measured by NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. One μg of RNA was reverse-transcribed into cDNA using the Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. cDNA Synthesis:Article Title: C-terminal interleukin 1 alpha (IL-1α) overexpression drives EMT and a vulnerability to ferroptosis in HNSCC Article Snippet: The RNA purity was measured by NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. One μg of RNA was reverse-transcribed into cDNA using the Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1 Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An Article Title: TNKS1 mediates the PTEN-PI3K/AKT pathway to regulate glycolysis and proliferation in gliomas Article Snippet: After 24 h of drug treatment, total RNA was extracted from cells using TRIzol reagent (15596026, Invitrogen, Carlsbad, CA, USA). .. Complementary DNA (cDNA) was then transcribed using the Article Title: Bone matrix-inspired whitlockite porous ceramic with effects of autophagy activation contributes to bone regeneration Article Snippet: TRIZOL (Thermo Fisher Scientific, USA) reagent was implemented to get total RNA as manufacturer guidelines. .. Conversion of total RNA to complementary DNA (cDNA) was carried out using an Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. Cell Culture:Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the Synthesized:Article Title: Hyperacetylation escalates myocardial susceptibility to ischemia reperfusion injury by mediating mitochondrial supercomplexes assembly in T2DM Article Snippet: The signals were quantitatively analyzed with ImageJ software (NIH), and the results were normalized to the GAPDH protein levels. .. Total RNA was extracted from heart tissues or cultured cardiomyocytes using TRIzol (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the Real-time Polymerase Chain Reaction:Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the Article Title: Time- and spatially resolved LNA delivery via thermally controlled SPION technology Article Snippet: .. For quantitative detection of mRNAs, reverse transcription of total RNA prior to real-time qPCR was performed using the Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1 Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An SYBR Green Assay:Article Title: Biomimetic de novo construction of hierarchically aligned and gradient-mineralized collagen for tendon-bone integrated regeneration Article Snippet: After 14 days of culture, cells were digested with 0.25% trypsin solution, and mRNA was extracted using TRIzol reagent (Bio-Rad, USA). .. Extracted mRNA was reverse transcribed into cDNA using the Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1 Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An Amplification:Article Title: The bovine mammosphere-derived epithelial cell secretome inhibits bacterial growth in vitro and contains peptidoglycan recognition protein 1 Article Snippet: Briefly, RNA was extracted from cells with a RNeasy Mini Plus kit (Qiagen, Germantown, MD) following manufacturer’s instructions. .. An Quantitative RT-PCR:Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. |