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Enzynomics co Ltd top scripttm cdna synthesis kit
Top Scripttm Cdna Synthesis Kit, supplied by Enzynomics co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+scripttm+reverse+transcriptase+cdna+synthesis+kit/top+scripttm+cdna+synthesis+kit/pm36008976-59-9-14
Average 90 stars, based on 1 article reviews
top scripttm cdna synthesis kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

cDNA Synthesis:

Article Title: Gene expression and biochemical response of giant reed under Ni and Cu stress.
Article Snippet: Giant reed (Arundo donax) has proved to be effective in detoxification, accumulation and tolerance of toxic metals.. The present study explored the stress response of giant reed against Cu and Ni stress.. The effect of metal stress was studied on dry weight, chlorophyll pigments antioxidant enzymes production and selected genes expression.

Article Title: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes.
Article Snippet: DNA was digested with DNase I, amplification grade (Life Technologies, Carlsbad, CA, USA). .. Reverse transcription and real-time PCR were performed using a Top scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea) and a Power SYBR® Green PCR Master Mix (Life Technologies, Warrington, UK), Downloaded from https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy091/4998850 by university of winnipeg user on 22 May 2018 respectively, according to the manufacturers’ instructions. ..

Article Title: Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition.
Article Snippet: First, 1 × 105 of HCT116 cells seeded in a 6-well plate were treated with either DMSO or GE and incubated for 24 h. Total RNA was purified using a GeneJET RNA purification kit (Thermo Scientific). .. From the purified RNA, cDNA was synthesized using a TOP scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea). .. All of the primers used in this study were obtained from Macrogen (Seoul, Korea) and are listed in Table S2.

Article Title: Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms.
Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 Download by: [60.173.133.229] Date: 05 November 2015, At: 13:36 Biofouling The Journal of Bioadhesion and Biofilm Research ISSN: 0892-7014 (Print) 1029-2454 (Online) Journal homepage: http://www.tandfonline.com/loi/gbif20 Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & JaeGyu Jeon To cite this article: Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & Jae-Gyu Jeon (2015) Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms, Biofouling, 31:7, 555-563, DOI: 10.1080/08927014.2015.1076799 To link to this article: http://dx.doi.org/10.1080/08927014.2015.1076799 Published online: 21 Aug 2015.. Submit your article to this journal Article views: 320 View related articles View Crossmark data Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Caia, Mi-A Kima, Ji-Eun Junga, Santosh Pandita, Kwang-Yeob Songb and Jae-Gyu Jeona,b* aDepartment of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Republic of Korea; bResearch Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea (Received 15 March 2015; accepted 22 July 2015) Despite the widespread use of fluoride, dental caries, a biofilm-related disease, remains an important health problem.. This study investigated whether oleic acid, a monounsaturated fatty acid, can enhance the effect of fluoride on extracellular polysaccharide (EPS) formation by Streptococcus mutans UA159 biofilms at sub-minimum inhibitory concentration levels, via microbiological and biochemical methods, confocal fluorescence microscopy, and real-time PCR.

Article Title: Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells.
Article Snippet: Extensive studies have been reported the non-canonical functions of pyruvate kinase M2 (PKM2) as kinase, transcriptional regulator and even cell-to-cell communicator, emphasizing its importance in various signaling pathways.. However, the role of secreted PKM2 in cancer progression and its signaling pathway is yet to be elucidated.. In this study, we found that extracellular PKM2 enhanced the migration of low-metastatic, benign colon cancer cells by upregulating claudin-1 expression and internalizing it to the cytoplasm and nucleus.

Reverse Transcription:

Article Title: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes.
Article Snippet: DNA was digested with DNase I, amplification grade (Life Technologies, Carlsbad, CA, USA). .. Reverse transcription and real-time PCR were performed using a Top scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea) and a Power SYBR® Green PCR Master Mix (Life Technologies, Warrington, UK), Downloaded from https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy091/4998850 by university of winnipeg user on 22 May 2018 respectively, according to the manufacturers’ instructions. ..

Article Title: Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms.
Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 Download by: [60.173.133.229] Date: 05 November 2015, At: 13:36 Biofouling The Journal of Bioadhesion and Biofilm Research ISSN: 0892-7014 (Print) 1029-2454 (Online) Journal homepage: http://www.tandfonline.com/loi/gbif20 Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & JaeGyu Jeon To cite this article: Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & Jae-Gyu Jeon (2015) Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms, Biofouling, 31:7, 555-563, DOI: 10.1080/08927014.2015.1076799 To link to this article: http://dx.doi.org/10.1080/08927014.2015.1076799 Published online: 21 Aug 2015.. Submit your article to this journal Article views: 320 View related articles View Crossmark data Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Caia, Mi-A Kima, Ji-Eun Junga, Santosh Pandita, Kwang-Yeob Songb and Jae-Gyu Jeona,b* aDepartment of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Republic of Korea; bResearch Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea (Received 15 March 2015; accepted 22 July 2015) Despite the widespread use of fluoride, dental caries, a biofilm-related disease, remains an important health problem.. This study investigated whether oleic acid, a monounsaturated fatty acid, can enhance the effect of fluoride on extracellular polysaccharide (EPS) formation by Streptococcus mutans UA159 biofilms at sub-minimum inhibitory concentration levels, via microbiological and biochemical methods, confocal fluorescence microscopy, and real-time PCR.

Real-time Polymerase Chain Reaction:

Article Title: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes.
Article Snippet: DNA was digested with DNase I, amplification grade (Life Technologies, Carlsbad, CA, USA). .. Reverse transcription and real-time PCR were performed using a Top scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea) and a Power SYBR® Green PCR Master Mix (Life Technologies, Warrington, UK), Downloaded from https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy091/4998850 by university of winnipeg user on 22 May 2018 respectively, according to the manufacturers’ instructions. ..

Article Title: Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms.
Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 Download by: [60.173.133.229] Date: 05 November 2015, At: 13:36 Biofouling The Journal of Bioadhesion and Biofilm Research ISSN: 0892-7014 (Print) 1029-2454 (Online) Journal homepage: http://www.tandfonline.com/loi/gbif20 Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & JaeGyu Jeon To cite this article: Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & Jae-Gyu Jeon (2015) Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms, Biofouling, 31:7, 555-563, DOI: 10.1080/08927014.2015.1076799 To link to this article: http://dx.doi.org/10.1080/08927014.2015.1076799 Published online: 21 Aug 2015.. Submit your article to this journal Article views: 320 View related articles View Crossmark data Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Caia, Mi-A Kima, Ji-Eun Junga, Santosh Pandita, Kwang-Yeob Songb and Jae-Gyu Jeona,b* aDepartment of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Republic of Korea; bResearch Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea (Received 15 March 2015; accepted 22 July 2015) Despite the widespread use of fluoride, dental caries, a biofilm-related disease, remains an important health problem.. This study investigated whether oleic acid, a monounsaturated fatty acid, can enhance the effect of fluoride on extracellular polysaccharide (EPS) formation by Streptococcus mutans UA159 biofilms at sub-minimum inhibitory concentration levels, via microbiological and biochemical methods, confocal fluorescence microscopy, and real-time PCR.

SYBR Green Assay:

Article Title: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes.
Article Snippet: DNA was digested with DNase I, amplification grade (Life Technologies, Carlsbad, CA, USA). .. Reverse transcription and real-time PCR were performed using a Top scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea) and a Power SYBR® Green PCR Master Mix (Life Technologies, Warrington, UK), Downloaded from https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy091/4998850 by university of winnipeg user on 22 May 2018 respectively, according to the manufacturers’ instructions. ..

Article Title: Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms.
Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 Download by: [60.173.133.229] Date: 05 November 2015, At: 13:36 Biofouling The Journal of Bioadhesion and Biofilm Research ISSN: 0892-7014 (Print) 1029-2454 (Online) Journal homepage: http://www.tandfonline.com/loi/gbif20 Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & JaeGyu Jeon To cite this article: Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & Jae-Gyu Jeon (2015) Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms, Biofouling, 31:7, 555-563, DOI: 10.1080/08927014.2015.1076799 To link to this article: http://dx.doi.org/10.1080/08927014.2015.1076799 Published online: 21 Aug 2015.. Submit your article to this journal Article views: 320 View related articles View Crossmark data Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Caia, Mi-A Kima, Ji-Eun Junga, Santosh Pandita, Kwang-Yeob Songb and Jae-Gyu Jeona,b* aDepartment of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Republic of Korea; bResearch Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea (Received 15 March 2015; accepted 22 July 2015) Despite the widespread use of fluoride, dental caries, a biofilm-related disease, remains an important health problem.. This study investigated whether oleic acid, a monounsaturated fatty acid, can enhance the effect of fluoride on extracellular polysaccharide (EPS) formation by Streptococcus mutans UA159 biofilms at sub-minimum inhibitory concentration levels, via microbiological and biochemical methods, confocal fluorescence microscopy, and real-time PCR.

Polymerase Chain Reaction:

Article Title: Sucrose challenges to Streptococcus mutans biofilms and the curve fitting for the biofilm changes.
Article Snippet: DNA was digested with DNase I, amplification grade (Life Technologies, Carlsbad, CA, USA). .. Reverse transcription and real-time PCR were performed using a Top scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea) and a Power SYBR® Green PCR Master Mix (Life Technologies, Warrington, UK), Downloaded from https://academic.oup.com/femsec/advance-article-abstract/doi/10.1093/femsec/fiy091/4998850 by university of winnipeg user on 22 May 2018 respectively, according to the manufacturers’ instructions. ..

Article Title: Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms.
Article Snippet: Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 Download by: [60.173.133.229] Date: 05 November 2015, At: 13:36 Biofouling The Journal of Bioadhesion and Biofilm Research ISSN: 0892-7014 (Print) 1029-2454 (Online) Journal homepage: http://www.tandfonline.com/loi/gbif20 Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & JaeGyu Jeon To cite this article: Jian-Na Cai, Mi-A Kim, Ji-Eun Jung, Santosh Pandit, Kwang-Yeob Song & Jae-Gyu Jeon (2015) Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms, Biofouling, 31:7, 555-563, DOI: 10.1080/08927014.2015.1076799 To link to this article: http://dx.doi.org/10.1080/08927014.2015.1076799 Published online: 21 Aug 2015.. Submit your article to this journal Article views: 320 View related articles View Crossmark data Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms Jian-Na Caia, Mi-A Kima, Ji-Eun Junga, Santosh Pandita, Kwang-Yeob Songb and Jae-Gyu Jeona,b* aDepartment of Preventive Dentistry, School of Dentistry, Institute of Oral Bioscience and BK 21 Plus Program, Chonbuk National University, Jeonju, Republic of Korea; bResearch Institute of Clinical Medicine of Chonbuk National University-Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea (Received 15 March 2015; accepted 22 July 2015) Despite the widespread use of fluoride, dental caries, a biofilm-related disease, remains an important health problem.. This study investigated whether oleic acid, a monounsaturated fatty acid, can enhance the effect of fluoride on extracellular polysaccharide (EPS) formation by Streptococcus mutans UA159 biofilms at sub-minimum inhibitory concentration levels, via microbiological and biochemical methods, confocal fluorescence microscopy, and real-time PCR.

Purification:

Article Title: Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition.
Article Snippet: First, 1 × 105 of HCT116 cells seeded in a 6-well plate were treated with either DMSO or GE and incubated for 24 h. Total RNA was purified using a GeneJET RNA purification kit (Thermo Scientific). .. From the purified RNA, cDNA was synthesized using a TOP scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea). .. All of the primers used in this study were obtained from Macrogen (Seoul, Korea) and are listed in Table S2.

Article Title: Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells.
Article Snippet: Extensive studies have been reported the non-canonical functions of pyruvate kinase M2 (PKM2) as kinase, transcriptional regulator and even cell-to-cell communicator, emphasizing its importance in various signaling pathways.. However, the role of secreted PKM2 in cancer progression and its signaling pathway is yet to be elucidated.. In this study, we found that extracellular PKM2 enhanced the migration of low-metastatic, benign colon cancer cells by upregulating claudin-1 expression and internalizing it to the cytoplasm and nucleus.

Synthesized:

Article Title: Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition.
Article Snippet: First, 1 × 105 of HCT116 cells seeded in a 6-well plate were treated with either DMSO or GE and incubated for 24 h. Total RNA was purified using a GeneJET RNA purification kit (Thermo Scientific). .. From the purified RNA, cDNA was synthesized using a TOP scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea). .. All of the primers used in this study were obtained from Macrogen (Seoul, Korea) and are listed in Table S2.

Article Title: Extracellular pyruvate kinase M2 facilitates cell migration by upregulating claudin-1 expression in colon cancer cells.
Article Snippet: Extensive studies have been reported the non-canonical functions of pyruvate kinase M2 (PKM2) as kinase, transcriptional regulator and even cell-to-cell communicator, emphasizing its importance in various signaling pathways.. However, the role of secreted PKM2 in cancer progression and its signaling pathway is yet to be elucidated.. In this study, we found that extracellular PKM2 enhanced the migration of low-metastatic, benign colon cancer cells by upregulating claudin-1 expression and internalizing it to the cytoplasm and nucleus.

other:

Article Title: Anti-inflammatory effects of a novel compound, MPQP, through the inhibition of IRAK1 signaling pathways in LPS-stimulated RAW 264.7 macrophages
Article Snippet: After LPS (100 ng/ml) stimulation for 3 h, total RNA was prepared from cells and reverse-transcribed into cDNA using a TOP-scriptTM cDNA synthesis kit (Enzynomics, Daejeon, Korea).



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Promega go scripttm reverse transcriptase cdna synthesis kit
Go Scripttm Reverse Transcriptase Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/go+scripttm+reverse+transcriptase+cdna+synthesis+kit/goscripttm+reverse+transcription+system/pmc09908614-84-21-27
Average 90 stars, based on 1 article reviews
go scripttm reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-10
90/100 stars
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