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ribo script mrna/lncrna qrt-pcr starter kit c11030-2  (Ribobio co)

 
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    Ribobio co ribo script mrna/lncrna qrt-pcr starter kit c11030-2
    Ribo Script Mrna/Lncrna Qrt Pcr Starter Kit C11030 2, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/go+script+reverse+transcriptase+kits/qpcr+starter+kit/pmc11228922-48-1-11
    Average 90 stars, based on 1 article reviews
    ribo script mrna/lncrna qrt-pcr starter kit c11030-2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    Reverse Transcription:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    Incubation:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    cDNA Synthesis:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    Real-time Polymerase Chain Reaction:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    Polymerase Chain Reaction:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).

    Expressing:

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRT-PCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mn 2+ -modified black phosphorus nanosensor for detection of exosomal microRNAs and exosomes.
    Article Snippet: The development and performance of a DNA probe adsorbing Mn2+-modified black phosphorus (BP@Mn2+/DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes.. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry.. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals.

    Article Title: Mechanism of YAP1 in the senescence and degeneration of endplate chondrocytes induced by intermittent cyclic mechanical tension.
    Article Snippet: A quantitative reverse transcription polymerase chain reaction (qRTPCR) starter kit (C11030, RiboBio, China) was used to synthesize first-strand cDNA from 2 μg RNA, and the qPCR conditions for the reaction protocol were as follows: stage 1, 95 °C for 5 min; stage 2, 95 °C for 5 s; stage 3, 60 °C for 30 s; and stage 4, 72 °C for 30 s. The relative expression levels of genes were normalized to the value of Gapdh using the ΔΔcycle threshold (ΔΔCq) method.

    Article Title: Mucin 1 expression is regulated by hsa_circ_0055054/microRNA‑122‑5p and promotes hepatocellular carcinoma development
    Article Snippet: The ribo SCRIPT mRNA/lncRNA qRT-PCR Starter Kit (cat. no. C11030-2; Guangzhou RiboBio Co., Ltd. was also employed; 1 μg of RNA template, 1 μl Random Primer, 1 μl Oligo (dT) 18 , 2 μl 5X reverse transcription buffer and 2 μl RTase Mix, made up to 10 μl using (RNase)-free double-distilled H 2 O and incubated at 42°C for 60 min and then 70°C for 10 min.

    Article Title: Connexin32 gap junction channels deliver miR155-3p to mediate pyroptosis in renal ischemia-reperfusion injury
    Article Snippet: Subsequently, a total of 10 ng RNA was used for cDNA synthesis and the RT-qPCR reactions were performed by C11030–1 riboSCRIPT mRNA/lncRNA RT-qPCR Starter Kit (RiboBio, China).

    Article Title: Identification and analysis of key circRNAs in the mouse embryonic ovary provides insight into primordial follicle development
    Article Snippet: Total RNA was reverse transcribed into cDNA, and qRT-PCR was performed using the qRT-PCR Starter Kit (RiboBio Co., Ltd, C11030-1) in a quantitative real-time PCR (qRT-PCR) instrument (QuantStudio TM3).



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