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go script™ reverse transcriptase cdna synthesis kit  (Promega)

 
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    Structured Review

    Promega go script™ reverse transcriptase cdna synthesis kit
    Go Script™ Reverse Transcriptase Cdna Synthesis Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/go+script+reverse+transcriptase+kits/reverse+transcription+kit/pmc07873859-181-17-23
    Average 90 stars, based on 1 article reviews
    go script™ reverse transcriptase cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    cDNA Synthesis:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    Article Title: ALKBH5 induces macrophage activation to promote renal fibrosis via Retnla
    Article Snippet: Quantitative, real-time RT-PCR total rNa was isolated using the trIzol rNa Isolation System (Invitrogen). .. First-strand cdNa synthesis was performed using a reverse transcription system kit, following the manufacturer’s instructions (Promega, Madison, WI). .. Quantitative real-time rt-PCr (qrt-PCr) was carried out on an abI PrISM 7000 Sequence detection System (applied biosystems, Foster City, Ca). the mrNa levels of target genes were normalized to β-actin for analysis and the primers are presented in Supplementary table 1.

    Reverse Transcription:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    Article Title: ALKBH5 induces macrophage activation to promote renal fibrosis via Retnla
    Article Snippet: Quantitative, real-time RT-PCR total rNa was isolated using the trIzol rNa Isolation System (Invitrogen). .. First-strand cdNa synthesis was performed using a reverse transcription system kit, following the manufacturer’s instructions (Promega, Madison, WI). .. Quantitative real-time rt-PCr (qrt-PCr) was carried out on an abI PrISM 7000 Sequence detection System (applied biosystems, Foster City, Ca). the mrNa levels of target genes were normalized to β-actin for analysis and the primers are presented in Supplementary table 1.

    Article Title: High-Fat Diet Differentially Regulates Fibroblast Growth Factor Expression in Metabolic Tissues of Young and Aged Male Mice
    Article Snippet: RNA concentration was determined with a Nanodrop ND-1000 spectrophotometer. .. For each sample, 1 μg of RNA was reverse transcribed into cDNA using a reverse transcription kit (Promega, Madison, WI). .. RT-qPCR was performed in duplicate on a LightCycler 96 system (Roche, Indianapolis, IN) using Power SYBR Green mix, with 18s rRNA as the housekeeping gene.

    Article Title: Efficacy of urolithin B-Loaded mesoporous silica nanoparticles modified with graphene quantum Dots against MCF-7 breast Cancer cells.
    Article Snippet: The quality and quantity of the extracted RNA were assessed using a ThermoFisher Nanodrop. .. Subsequently, complementary DNA (cDNA) was synthesized from 1 μg of RNA using a reverse transcription kit (Promega). ..

    Article Title: HSP90B1 facilitates glioma radiotherapy resistance by regulating RhoC ubiquitin‒proteasome degradation
    Article Snippet: Following the respective user manual, we isolated and purified total cellular RNA using the RNAExpress Total RNA Kit (NCM Biotech). .. With the Promega reverse transcription kit, we synthesized cDNA from mRNA through reverse transcription reactions. .. The CFX96 Real-Time System (Bio-Rad) was employed to quantify mRNA expression.

    Real-time Polymerase Chain Reaction:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    SYBR Green Assay:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    Control:

    Article Title: Tripartite motif 47 promotes the development of thyroid carcinoma through ADAR ubiquitination.
    Article Snippet: .. The RNA concentration was adjusted to 1 μg/μL, aliquoted, and stored at − 80 °C. cDNA synthesis was performed using the Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. qPCR was carried out using IQTM SYBR Green Supermix (Bio-Rad, Berkeley, USA) on a Mastercycler® ep realplex (Eppendorf, Hamburg, Germany) with GAPDH as the endogenous control. ..

    Synthesized:

    Article Title: Efficacy of urolithin B-Loaded mesoporous silica nanoparticles modified with graphene quantum Dots against MCF-7 breast Cancer cells.
    Article Snippet: The quality and quantity of the extracted RNA were assessed using a ThermoFisher Nanodrop. .. Subsequently, complementary DNA (cDNA) was synthesized from 1 μg of RNA using a reverse transcription kit (Promega). ..

    Article Title: HSP90B1 facilitates glioma radiotherapy resistance by regulating RhoC ubiquitin‒proteasome degradation
    Article Snippet: Following the respective user manual, we isolated and purified total cellular RNA using the RNAExpress Total RNA Kit (NCM Biotech). .. With the Promega reverse transcription kit, we synthesized cDNA from mRNA through reverse transcription reactions. .. The CFX96 Real-Time System (Bio-Rad) was employed to quantify mRNA expression.

    other:

    Article Title: Cardiac fibrosis inhibitor CTPR390 prevents structural and morphological changes in human engineered cardiac connective tissue
    Article Snippet: Complementary DNA was prepared from 0.5 μg total RNA by random priming using a first-strand cDNA synthesis kit (Promega Corp).

    Article Title: Engineered ligase variants
    Article Snippet: Products were cleaned using the Wizard® SV PCR cleanup kit (Promega) and eluted in water.

    Polymerase Chain Reaction:

    Article Title: Characterization of glutamine synthetase involved in the fecundity of Rhopalosiphum padi.
    Article Snippet: .. The PCR products were purified from 1% agarose gels by the Wizard PCR Preps Kit (Promega, WI, USA). .. The purified fragment was subsequently cloned and inserted into a pMD19-T vector (Takara, Beijing, China) and transformed into Escherichia coli DH5α-competent cells.

    Purification:

    Article Title: Characterization of glutamine synthetase involved in the fecundity of Rhopalosiphum padi.
    Article Snippet: .. The PCR products were purified from 1% agarose gels by the Wizard PCR Preps Kit (Promega, WI, USA). .. The purified fragment was subsequently cloned and inserted into a pMD19-T vector (Takara, Beijing, China) and transformed into Escherichia coli DH5α-competent cells.



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