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script cdna synthesis kit  (Jena Bioscience)


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    Jena Bioscience script cdna synthesis kit
    Script Cdna Synthesis Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 225 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/go+script+reverse+transcriptase+kits/SCRIPT+cDNA+Synthesis+Kit/pm29520026-207-37-41
    Average 96 stars, based on 225 article reviews
    script cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Prevalence, Clinical Signs, Diagnosis and Treatment of Post-Pandemic SARS-CoV-2 Infection in Cats in 2023: Co-Infection with FHV, FCV, Mycoplasma spp. and Chlamydia felis —A Single-Center Study in Bulgaria
    Article Snippet: To obtain DNA/RNA from clinical samples and cell cultures, we used a viral RNA/DNA extraction kit (Jena Bioscience, Jena, Germany) according to the manufacturer’s instructions.

    Article Title: Recolonization dynamics of the middle ear microbiota following MESNA-assisted dissection in pediatric cholesteatomatous chronic otitis media
    Article Snippet: A uniform input of 1 μg total RNA per reaction was used for cDNA synthesis with random hexamers (SCRIPT cDNA Synthesis Kit, Jena Bioscience), ensuring comparable cDNA inputs across all samples.

    Article Title: Modulation of Drug Resistance and Apoptotic Pathways Underlies the Enhanced Antitumor Effect of Ellagic Acid-Irinotecan Combination in Glioma.
    Article Snippet: Caspase-3 immunostaining was performed to assess protein-level apoptotic activation, using anti-caspase-3 antibody (AB3623; Millipore, Darmstadt, Germany) applied overnight at 4 ◦C.

    cDNA Synthesis:

    Article Title: Chromatin accessibility classification of TAD boundaries discloses new architectural proteins
    Article Snippet: The RNAs were treated with RQ1 RNase-free DNase (Promega). .. The complementary DNA (cDNA) synthesis were performed with SCRIPT cDNA Synthesis Kit (Jena Bioscience). ..

    Article Title: Trichomonas vaginalis cathepsin D-like aspartic proteinase (Tv-CatD) is regulated by iron at different levels.
    Article Snippet: RT‒qPCR assays Total RNA was obtained from 2 × 107 PBS‒washed parasites grown under IR and HI conditions using TRIzol (Invitrogen). .. cDNA was synthesized using the SCRIPT cDNA Synthesis Kit (Jena Bioscience) following the manufacturer’s instructions. .. RT‒qPCR was performed using PanGreen Universal SYBR Green Master Mix (Bio-Helix) with 150 ng of cDNA from the IR or HI conditions as a template and 500 nM specific tv-catd primers (sense: 5′-GAA CAT CTC CGT TCA GGG CA-3′; antisense: 5′-AGC ATC TGG GTT GTA GCC AC-3′) to amplify a 145 bp fragment.

    Article Title: NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.
    Article Snippet: .. The RNA integrity was validated by visual inspection of RNA bands on agarose gel electrophoresis. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S). qPCRs were carried out using the qPCR Green Master Mix with UNG/High ROX kit (PCR-374S). .. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S).

    Synthesized:

    Article Title: Trichomonas vaginalis cathepsin D-like aspartic proteinase (Tv-CatD) is regulated by iron at different levels.
    Article Snippet: RT‒qPCR assays Total RNA was obtained from 2 × 107 PBS‒washed parasites grown under IR and HI conditions using TRIzol (Invitrogen). .. cDNA was synthesized using the SCRIPT cDNA Synthesis Kit (Jena Bioscience) following the manufacturer’s instructions. .. RT‒qPCR was performed using PanGreen Universal SYBR Green Master Mix (Bio-Helix) with 150 ng of cDNA from the IR or HI conditions as a template and 500 nM specific tv-catd primers (sense: 5′-GAA CAT CTC CGT TCA GGG CA-3′; antisense: 5′-AGC ATC TGG GTT GTA GCC AC-3′) to amplify a 145 bp fragment.

    Process/Product Development:

    Article Title: NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.
    Article Snippet: .. The RNA integrity was validated by visual inspection of RNA bands on agarose gel electrophoresis. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S). qPCRs were carried out using the qPCR Green Master Mix with UNG/High ROX kit (PCR-374S). .. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S).

    Agarose Gel Electrophoresis:

    Article Title: NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.
    Article Snippet: .. The RNA integrity was validated by visual inspection of RNA bands on agarose gel electrophoresis. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S). qPCRs were carried out using the qPCR Green Master Mix with UNG/High ROX kit (PCR-374S). .. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S).

    Polymerase Chain Reaction:

    Article Title: NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.
    Article Snippet: .. The RNA integrity was validated by visual inspection of RNA bands on agarose gel electrophoresis. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S). qPCRs were carried out using the qPCR Green Master Mix with UNG/High ROX kit (PCR-374S). .. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S).

    Real-time Polymerase Chain Reaction:

    Article Title: NR5A2 controls gene expression and chromatin contacts of essential circadian metabolic genes in the liver.
    Article Snippet: .. The RNA integrity was validated by visual inspection of RNA bands on agarose gel electrophoresis. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S). qPCRs were carried out using the qPCR Green Master Mix with UNG/High ROX kit (PCR-374S). .. cDNA synthesis was performed using random primers with the Jena Bioscience SCRIPT RT cDNA synthesis kit (PCR-511S).



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