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Promega glosensor-22f vector
Glosensor 22f Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor-22f+vector/22f+plasmid/pm26456674-71-6-10
Average 90 stars, based on 1 article reviews
glosensor-22f vector - by Bioz Stars, 2026-10
90/100 stars

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Transfection:

Article Title: Role of Helix 8 in Dopamine Receptor Signaling
Article Snippet: HEK293 cells were cultivated in DMEM medium supplemented with 1% penicillin-streptomycin solution (Wellgene, Gyeongsan, Korea) and FBS (10% v/v) at 37°C in a humidified atmosphere with 5% CO 2 . .. Early passage HEK293 cells were seeded in a 6-well plate, and cells were transfected with 1 μg of 22F plasmid (Promega, WI, USA). .. Positive clones were selected using hygromycin B (200 μg/mL), and stable expression of 22F was determined using the Glo-Sensor TM cAMP assay kit (Promega) according to the manufacturer’s instructions.

Article Title: Tirzepatide is an imbalanced and biased dual GIP and GLP-1 receptor agonist
Article Snippet: Normalized percent values were fit to the 4-parameter logistic model in Genedata Screener or GraphPad Prism 7 software. .. Kinetic cAMP assays were performed in low-density GIPR and GLP-1R cell clones transfected with the Glosensor 22F vector (Promega) ( ). ..

Article Title: Different contributions of chemokine N‐terminal features attest to a different ligand binding mode and a bias towards activation of ACKR3/CXCR7 compared with CXCR4 and CXCR3
Article Snippet: .. U87 cells stably transfected with cAMP GloSensor 22F vector (Promega, Madison, WI) (U87.Glo) were selected using hygromycin resistance (10 μg·mL −1 ), and the forskolin‐induced luminescence response was assessed. ..

Plasmid Preparation:

Article Title: Role of Helix 8 in Dopamine Receptor Signaling
Article Snippet: HEK293 cells were cultivated in DMEM medium supplemented with 1% penicillin-streptomycin solution (Wellgene, Gyeongsan, Korea) and FBS (10% v/v) at 37°C in a humidified atmosphere with 5% CO 2 . .. Early passage HEK293 cells were seeded in a 6-well plate, and cells were transfected with 1 μg of 22F plasmid (Promega, WI, USA). .. Positive clones were selected using hygromycin B (200 μg/mL), and stable expression of 22F was determined using the Glo-Sensor TM cAMP assay kit (Promega) according to the manufacturer’s instructions.

Article Title: Tirzepatide is an imbalanced and biased dual GIP and GLP-1 receptor agonist
Article Snippet: Normalized percent values were fit to the 4-parameter logistic model in Genedata Screener or GraphPad Prism 7 software. .. Kinetic cAMP assays were performed in low-density GIPR and GLP-1R cell clones transfected with the Glosensor 22F vector (Promega) ( ). ..

Article Title: Different contributions of chemokine N‐terminal features attest to a different ligand binding mode and a bias towards activation of ACKR3/CXCR7 compared with CXCR4 and CXCR3
Article Snippet: .. U87 cells stably transfected with cAMP GloSensor 22F vector (Promega, Madison, WI) (U87.Glo) were selected using hygromycin resistance (10 μg·mL −1 ), and the forskolin‐induced luminescence response was assessed. ..

Article Title: Distinct phosphorylation sites in a prototypical GPCR differently orchestrate β-arrestin interaction, trafficking, and signaling
Article Snippet: .. For measuring cAMP response for V 2 R constructs, HEK-293 cells were cotransfected with the indicated receptor construct and 22F plasmid (Promega). .. Twenty-four hours after transfection, cells were detached from the plates, centrifuged, and resuspended in buffer [1× HBSS supplemented with 20 mM Hepes (pH 7.4)] containing luciferin (0.5 mg/ml; GoldBio).

Clone Assay:

Article Title: Tirzepatide is an imbalanced and biased dual GIP and GLP-1 receptor agonist
Article Snippet: Normalized percent values were fit to the 4-parameter logistic model in Genedata Screener or GraphPad Prism 7 software. .. Kinetic cAMP assays were performed in low-density GIPR and GLP-1R cell clones transfected with the Glosensor 22F vector (Promega) ( ). ..

Stable Transfection:

Article Title: Different contributions of chemokine N‐terminal features attest to a different ligand binding mode and a bias towards activation of ACKR3/CXCR7 compared with CXCR4 and CXCR3
Article Snippet: .. U87 cells stably transfected with cAMP GloSensor 22F vector (Promega, Madison, WI) (U87.Glo) were selected using hygromycin resistance (10 μg·mL −1 ), and the forskolin‐induced luminescence response was assessed. ..

Construct:

Article Title: Distinct phosphorylation sites in a prototypical GPCR differently orchestrate β-arrestin interaction, trafficking, and signaling
Article Snippet: .. For measuring cAMP response for V 2 R constructs, HEK-293 cells were cotransfected with the indicated receptor construct and 22F plasmid (Promega). .. Twenty-four hours after transfection, cells were detached from the plates, centrifuged, and resuspended in buffer [1× HBSS supplemented with 20 mM Hepes (pH 7.4)] containing luciferin (0.5 mg/ml; GoldBio).

other:

Article Title: Partial ligand-receptor engagement yields functional bias at the human complement receptor, C5aR1
Article Snippet: 50–60% confluent HEK-293 cells were co-transfected with 3.5 μg each of C5aR1 or C5a-V2R and 22F (Promega) plasmids.

Article Title: Differences in the Antinociceptive Effects and Binding Properties of Propranolol and Bupranolol Enantiomers.
Article Snippet: Received 2015.. This rese (J.S.M.).. L dian Inst J.S.M. is founders providin tics.



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