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glosensor camp kit  (Promega)

 
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    Promega glosensor camp kit
    Glosensor Camp Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/glosensor+kit/glosensor+camp+assay/pm40670604-246-1-5
    Average 90 stars, based on 1 article reviews
    glosensor camp kit - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    cAMP Assay:

    Article Title: Compositions and methods for anti-PACAP antibodies
    Article Snippet: .. In addition, the antibody-independent GloSensor cAMP assay from Promega employs semi-split luciferase, which reassembles when bound to CAMP. ..

    Luciferase:

    Article Title: Compositions and methods for anti-PACAP antibodies
    Article Snippet: .. In addition, the antibody-independent GloSensor cAMP assay from Promega employs semi-split luciferase, which reassembles when bound to CAMP. ..

    Transfection:

    Article Title: Molecular basis for ligand recognition and receptor activation of the prostaglandin D2 receptor DP1.
    Article Snippet: After overnight growth, the cells were transfected with a plasmid mixture consisting of the DP1/EP2/EP4 construct and the cAMP biosensor GloSensor22F (Promega) at a 1:1 ratio. .. Following 24 h of transfection, the cells were adapted to CO2- independent media containing 2% GloSensor cAMP Reagent (Promega) and plated onto a 384- well assay plate at a density of 4,000 cells/10 μL/well. ..

    other:

    Article Title: Discovery of a functionally selective serotonin receptor (5-HT 1A R) agonist for the treatment of pain.
    Article Snippet: Cells with a confluency of 60 to 80% (10- cm culture dish) were transfected with 0.5 μg (HEK293A, HEK293T, and HEK293AΔARRB1/B2) or 1 μg of 5- HT1AR plasmid DNA (HEK293AΔGs and HEK293AΔGi/o), 0.5 μg (HEK293A, HEK293T, and HEK293AΔARRB1/B2) or 2 μg (HEK293AΔGs and HEKS293AΔGi/o) of Glo- 22F cAMP plasmid DNA (Promega), and 2 μg of MOCK DNA (HEK293A, HEK293T, and HEK293AΔARRB1/B2).

    Plasmid Preparation:

    Article Title: Pharmacological modulation of MRAP2 protein on murine melatonin receptor signaling
    Article Snippet: .. The pGloSensor-22F cAMP plasmid and GloSensor cAMP reagent were procured from Promega (Madison, WI, USA), while melatonin was synthesized by MedChemExpress (Monmouth Junction, NJ, USA). .. Hydrochloric acid and sulfuric acid were sourced from Sinopharm Chemical Reagent Co., Ltd (Beijing, China).

    Synthesized:

    Article Title: Pharmacological modulation of MRAP2 protein on murine melatonin receptor signaling
    Article Snippet: .. The pGloSensor-22F cAMP plasmid and GloSensor cAMP reagent were procured from Promega (Madison, WI, USA), while melatonin was synthesized by MedChemExpress (Monmouth Junction, NJ, USA). .. Hydrochloric acid and sulfuric acid were sourced from Sinopharm Chemical Reagent Co., Ltd (Beijing, China).

    Incubation:

    Article Title: Discovery of a functionally selective serotonin receptor (5-HT 1A R) agonist for the treatment of pain
    Article Snippet: .. After incubation, CP4 medium was exchanged with Hanks’ balanced salt solution [with added glucose (1 g/liter) and NaHCO 3 (0.35 g/liter)] containing GloSensor cAMP Reagent (3%, Promega) and plates were incubated for 60 min. ..



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    FUT8 knockdown attenuates the FSH/FSHR signaling pathway. A, CCK8 cell proliferation assay. Cells were treated with 100 ng/mL rhFSH, and cell proliferation was measured by absorbance at a wavelength of 450 nm. Significant differences are shown as the means ± SD, n = 4. B, Immunofluorescence assay. KGN, KGN-KD and KGN-KD-Re cells were treated with FSH (400 ng/mL) and stained with anti-FSHR (red) (1:500) and DAPI (blue). C, Western blot. Cell lysates of KGN, KGN-KD and KGN-KD-Re cells were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as the reference gene for the cytoplasm. D, Western blot. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as a loading control. E, Quantitative real-time PCR. RNA was isolated from the granulosa cells of MOR and POR patients. The mRNA expression levels of FSHR were compared. The relative expression of mRNAs was normalized to GAPDH. Data are presented as the means ± SEM, n = 3. F, FSH-induced cAMP production was measured using a <t>GloSensor</t> cAMP Assay kit. Cells were seeded in a 6-well plate for 24 h, and then 100 μM of IBMX was added, to inhibit phosphodiesterases, for 20 min. The cAMP levels were measured in KGN, KGN-KD, and KGN-KD-Re cells following stimulation with 100 IU/L of rhFSH or 100 μM of forskolin for 5 min. Data are presented as the means ± SEM, n = 3. G, PKA-CREB signaling pathway and Akt-FoxO3a signaling pathway. Serum-starved KGN, KGN-KD, and KGN-KD-Re cells were treated with FSH for 30 min, and cell lysates were resolved by 10 % SDS-PAGE, followed by transfer to a PVDF membrane. Membranes were probed with anti-phospho-PKA (1:2000) and anti-PKA (1:2000); anti-phospho-CREB (1:2000) and anti-CREB (1:2000); and anti-KITL (1:2000); anti-phospho-Akt (1:2000) and anti-Akt (1:2000); anti-phospho-FoxO3a (1:2000) and anti-FoxO3a (1:2000), and GAPDH was used as a loading control. Quantitative data are represented as the means ± SD, n = 3. H, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE, and probed with anti-KITL (1:2000), with GAPDH used as the loading control. Quantitative data are represented as the means ± SD, n = 3. I, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE and probed with anti-pFoxO3a (1:2000) and anti-FoxO3a (1:2000). Quantitative data are represented as the means ± SD, n = 3. J, TUNEL assay in the Fut8 +/+ and Fut8 −/− ovarian follicles. Representative micrographs are shown. Quantitative data are represented as the means ± SD, n = 3. K, Western blot analysis. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane, and probed with anti-BCL2 and anti-BAX (1:2000). Quantitative data are represented as the means ± SD, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    FUT8 knockdown attenuates the FSH/FSHR signaling pathway. A, CCK8 cell proliferation assay. Cells were treated with 100 ng/mL rhFSH, and cell proliferation was measured by absorbance at a wavelength of 450 nm. Significant differences are shown as the means ± SD, n = 4. B, Immunofluorescence assay. KGN, KGN-KD and KGN-KD-Re cells were treated with FSH (400 ng/mL) and stained with anti-FSHR (red) (1:500) and DAPI (blue). C, Western blot. Cell lysates of KGN, KGN-KD and KGN-KD-Re cells were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as the reference gene for the cytoplasm. D, Western blot. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as a loading control. E, Quantitative real-time PCR. RNA was isolated from the granulosa cells of MOR and POR patients. The mRNA expression levels of FSHR were compared. The relative expression of mRNAs was normalized to GAPDH. Data are presented as the means ± SEM, n = 3. F, FSH-induced cAMP production was measured using a <t>GloSensor</t> cAMP Assay kit. Cells were seeded in a 6-well plate for 24 h, and then 100 μM of IBMX was added, to inhibit phosphodiesterases, for 20 min. The cAMP levels were measured in KGN, KGN-KD, and KGN-KD-Re cells following stimulation with 100 IU/L of rhFSH or 100 μM of forskolin for 5 min. Data are presented as the means ± SEM, n = 3. G, PKA-CREB signaling pathway and Akt-FoxO3a signaling pathway. Serum-starved KGN, KGN-KD, and KGN-KD-Re cells were treated with FSH for 30 min, and cell lysates were resolved by 10 % SDS-PAGE, followed by transfer to a PVDF membrane. Membranes were probed with anti-phospho-PKA (1:2000) and anti-PKA (1:2000); anti-phospho-CREB (1:2000) and anti-CREB (1:2000); and anti-KITL (1:2000); anti-phospho-Akt (1:2000) and anti-Akt (1:2000); anti-phospho-FoxO3a (1:2000) and anti-FoxO3a (1:2000), and GAPDH was used as a loading control. Quantitative data are represented as the means ± SD, n = 3. H, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE, and probed with anti-KITL (1:2000), with GAPDH used as the loading control. Quantitative data are represented as the means ± SD, n = 3. I, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE and probed with anti-pFoxO3a (1:2000) and anti-FoxO3a (1:2000). Quantitative data are represented as the means ± SD, n = 3. J, TUNEL assay in the Fut8 +/+ and Fut8 −/− ovarian follicles. Representative micrographs are shown. Quantitative data are represented as the means ± SD, n = 3. K, Western blot analysis. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane, and probed with anti-BCL2 and anti-BAX (1:2000). Quantitative data are represented as the means ± SD, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    FUT8 knockdown attenuates the FSH/FSHR signaling pathway. A, CCK8 cell proliferation assay. Cells were treated with 100 ng/mL rhFSH, and cell proliferation was measured by absorbance at a wavelength of 450 nm. Significant differences are shown as the means ± SD, n = 4. B, Immunofluorescence assay. KGN, KGN-KD and KGN-KD-Re cells were treated with FSH (400 ng/mL) and stained with anti-FSHR (red) (1:500) and DAPI (blue). C, Western blot. Cell lysates of KGN, KGN-KD and KGN-KD-Re cells were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as the reference gene for the cytoplasm. D, Western blot. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as a loading control. E, Quantitative real-time PCR. RNA was isolated from the granulosa cells of MOR and POR patients. The mRNA expression levels of FSHR were compared. The relative expression of mRNAs was normalized to GAPDH. Data are presented as the means ± SEM, n = 3. F, FSH-induced cAMP production was measured using a GloSensor cAMP Assay kit. Cells were seeded in a 6-well plate for 24 h, and then 100 μM of IBMX was added, to inhibit phosphodiesterases, for 20 min. The cAMP levels were measured in KGN, KGN-KD, and KGN-KD-Re cells following stimulation with 100 IU/L of rhFSH or 100 μM of forskolin for 5 min. Data are presented as the means ± SEM, n = 3. G, PKA-CREB signaling pathway and Akt-FoxO3a signaling pathway. Serum-starved KGN, KGN-KD, and KGN-KD-Re cells were treated with FSH for 30 min, and cell lysates were resolved by 10 % SDS-PAGE, followed by transfer to a PVDF membrane. Membranes were probed with anti-phospho-PKA (1:2000) and anti-PKA (1:2000); anti-phospho-CREB (1:2000) and anti-CREB (1:2000); and anti-KITL (1:2000); anti-phospho-Akt (1:2000) and anti-Akt (1:2000); anti-phospho-FoxO3a (1:2000) and anti-FoxO3a (1:2000), and GAPDH was used as a loading control. Quantitative data are represented as the means ± SD, n = 3. H, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE, and probed with anti-KITL (1:2000), with GAPDH used as the loading control. Quantitative data are represented as the means ± SD, n = 3. I, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE and probed with anti-pFoxO3a (1:2000) and anti-FoxO3a (1:2000). Quantitative data are represented as the means ± SD, n = 3. J, TUNEL assay in the Fut8 +/+ and Fut8 −/− ovarian follicles. Representative micrographs are shown. Quantitative data are represented as the means ± SD, n = 3. K, Western blot analysis. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane, and probed with anti-BCL2 and anti-BAX (1:2000). Quantitative data are represented as the means ± SD, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Core fucosylation regulates the ovarian response via FSH receptor during follicular development

    doi: 10.1016/j.jare.2024.01.025

    Figure Lengend Snippet: FUT8 knockdown attenuates the FSH/FSHR signaling pathway. A, CCK8 cell proliferation assay. Cells were treated with 100 ng/mL rhFSH, and cell proliferation was measured by absorbance at a wavelength of 450 nm. Significant differences are shown as the means ± SD, n = 4. B, Immunofluorescence assay. KGN, KGN-KD and KGN-KD-Re cells were treated with FSH (400 ng/mL) and stained with anti-FSHR (red) (1:500) and DAPI (blue). C, Western blot. Cell lysates of KGN, KGN-KD and KGN-KD-Re cells were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as the reference gene for the cytoplasm. D, Western blot. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane and probed with anti-FSHR (1:2000). GAPDH was used as a loading control. E, Quantitative real-time PCR. RNA was isolated from the granulosa cells of MOR and POR patients. The mRNA expression levels of FSHR were compared. The relative expression of mRNAs was normalized to GAPDH. Data are presented as the means ± SEM, n = 3. F, FSH-induced cAMP production was measured using a GloSensor cAMP Assay kit. Cells were seeded in a 6-well plate for 24 h, and then 100 μM of IBMX was added, to inhibit phosphodiesterases, for 20 min. The cAMP levels were measured in KGN, KGN-KD, and KGN-KD-Re cells following stimulation with 100 IU/L of rhFSH or 100 μM of forskolin for 5 min. Data are presented as the means ± SEM, n = 3. G, PKA-CREB signaling pathway and Akt-FoxO3a signaling pathway. Serum-starved KGN, KGN-KD, and KGN-KD-Re cells were treated with FSH for 30 min, and cell lysates were resolved by 10 % SDS-PAGE, followed by transfer to a PVDF membrane. Membranes were probed with anti-phospho-PKA (1:2000) and anti-PKA (1:2000); anti-phospho-CREB (1:2000) and anti-CREB (1:2000); and anti-KITL (1:2000); anti-phospho-Akt (1:2000) and anti-Akt (1:2000); anti-phospho-FoxO3a (1:2000) and anti-FoxO3a (1:2000), and GAPDH was used as a loading control. Quantitative data are represented as the means ± SD, n = 3. H, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE, and probed with anti-KITL (1:2000), with GAPDH used as the loading control. Quantitative data are represented as the means ± SD, n = 3. I, Western blot. Lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 10 % SDS-PAGE and probed with anti-pFoxO3a (1:2000) and anti-FoxO3a (1:2000). Quantitative data are represented as the means ± SD, n = 3. J, TUNEL assay in the Fut8 +/+ and Fut8 −/− ovarian follicles. Representative micrographs are shown. Quantitative data are represented as the means ± SD, n = 3. K, Western blot analysis. Cell lysates of Fut8 +/+ and Fut8 −/− ovaries were resolved by 7.5 % SDS-PAGE, transferred to a PVDF membrane, and probed with anti-BCL2 and anti-BAX (1:2000). Quantitative data are represented as the means ± SD, n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: FSH-induced cAMP production was measured using a GloSensor cAMP Assay kit (Promega).

    Techniques: Knockdown, Proliferation Assay, Immunofluorescence, Staining, Western Blot, SDS Page, Membrane, Control, Real-time Polymerase Chain Reaction, Isolation, Expressing, cAMP Assay, TUNEL Assay