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    Qiagen assays qiaamp dna blood mini kit qiagen
    Assays Qiaamp Dna Blood Mini Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/global+screening+array+dna+microarray+chips/QIAamp+DNA+Blood+Mini+Kit/pm30526881-128-80-86
    Average 99 stars, based on 3362 article reviews
    assays qiaamp dna blood mini kit qiagen - by Bioz Stars, 2026-10
    99/100 stars

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    Sequencing:

    Article Title: Meiotic gene variants contribute to recurrent blastulation failure
    Article Snippet: .. 166 Whole-exome sequencing and variant analysis 167 Genomic DNA was extracted from peripheral blood samples using standard procedures 168 (Qiagen, Hilden, Germany, 51106). .. Whole-exome capture followed by sequencing was https://mc.manuscriptcentral.com/hropen Manuscripts submitted to Human Reproduction Open D ow nloaded from https://academ ic.oup.com /hropen/advance-article/doi/10.1093/hropen/hoag039/8675487 by guest on 19 M ay 2026 169 conducted on the Illumina NovaSeq 6000 platform.

    Variant Assay:

    Article Title: Meiotic gene variants contribute to recurrent blastulation failure
    Article Snippet: .. 166 Whole-exome sequencing and variant analysis 167 Genomic DNA was extracted from peripheral blood samples using standard procedures 168 (Qiagen, Hilden, Germany, 51106). .. Whole-exome capture followed by sequencing was https://mc.manuscriptcentral.com/hropen Manuscripts submitted to Human Reproduction Open D ow nloaded from https://academ ic.oup.com /hropen/advance-article/doi/10.1093/hropen/hoag039/8675487 by guest on 19 M ay 2026 169 conducted on the Illumina NovaSeq 6000 platform.

    Recombinant:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    Protease Inhibitor:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Purification:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Plasmid Preparation:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Gel Extraction:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Mass Spectrometry:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    RNA Sequencing:

    Article Title: Presentation of identical tumor antigen peptides by canine and human MHC class I
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MHC Class I (HLA-A, HLA-B, HLA-C) – human, Clone W6/32 BioXCell Cat# BE0079; RRID: AB_1107730 Mouse anti-MHC class I monoclonal antibody, Clone DG-H58A Monoclonal Antibody Center, Washington State University Cat# DG-BOV2001; RRID: AB_3718131 Anti-Human HLA A2, B7 (MHC Class I), Clone BB7.6 Leinco Technologies RRID: AB_2893725 Bacterial and virus strains OneShot Top10 Chemically Competent ThermoFisher Cat# C404003 Biological samples Canine tumor samples Collected from veterinary hospitals with owner informed consent. .. N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 11875119 DMEM/F-12 Gibco Cat# 11320082 PBS Gibco Cat# 20012027 FBS Gibco Cat# A5256701 CNBr-activated Sepharose 4 Fast Flow Amersham Pharmacia Biotech/Cytiva Cat# 17-0981-01 Tris pH 8.0 NELS Cat# E199-500 ML IGEPAL® CA-630 Sigma-Aldrich Cat# 18896 Complete protease inhibitor cocktail Roche Cat# 11836145001 Glacial Acetic acid Sigma-Aldrich Cat# AX0077 iRT peptides Biognosys Cat# 1900615 Transporter 5 Polysciences Cat# 26008 Xho1 NEB Cat# R0146S Salt-T4 DNA Ligase NEB Cat# M0467S EcoR1 NEB Cat# R3101S LR Clonase II ThermoFisher Cat# 11791020 Polybrene Sigma-Aldrich Cat# TR-1003-50UL Blasticidin ThermoFisher Cat# A1113903 Critical commercial assays MelonTM Gel IgG Spin Purification Kit Thermo Scientific Cat# 45206 ZebaTM Spin Desalting Columns Thermo Scientific Cat# 89893 QIAfilter Plasmid Kits Qiagen Cat #12243 Monarch Plasmid Spin Miniprep Kit NEB Cat #T1110L Monarch Spin DNA Gel Extraction Kit NEB Cat #T1120L QIAamp DNA Blood Mini Kit Qiagen Cat #51106 Rapid Barcoding Kit 96 V14 Oxford Nanopore Technologies, UK Cat #SQK-RBK114.96 Deposited data Mass spectrometry data for DLAtransduced mono-allelic cells and all canine tumors This paper PXD074485 Canine tumors RNA-seq data The SRA database PRJNA1428288 (Continued on next page) e1 iScience 29, 115975, June 19, 2026 ..

    Isolation:

    Article Title: Heparanase (HPSE) genetic variants as prognostic indicators in ovarian cancer: evidence from discovery and validation cohorts
    Article Snippet: .. DNA was isolated using the QIAamp DNA Blood Mini Kit (Cat. No. 51106, Qiagen, Hilden, Germany), and RNA was extracted from peripheral blood components (PBCs) using the GRS RNA kit - Blood & cultured cells (#GK08.0100, Grisp Research Resolutions ® , Porto, Portugal), following manufacturers’ protocols. .. Nucleic acid concentration and sample purity were confirmed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

    Cell Culture:

    Article Title: Heparanase (HPSE) genetic variants as prognostic indicators in ovarian cancer: evidence from discovery and validation cohorts
    Article Snippet: .. DNA was isolated using the QIAamp DNA Blood Mini Kit (Cat. No. 51106, Qiagen, Hilden, Germany), and RNA was extracted from peripheral blood components (PBCs) using the GRS RNA kit - Blood & cultured cells (#GK08.0100, Grisp Research Resolutions ® , Porto, Portugal), following manufacturers’ protocols. .. Nucleic acid concentration and sample purity were confirmed using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

    RNA Extraction:

    Article Title: Comprehensive Molecular Characterization of High-Grade Endometrial Cancer in an Ancestrally Diverse Cohort
    Article Snippet: The isolated PBMCs were cryopreserved following resuspension in Recovery Cell Culture Freezing Medium (ThermoFisher, 12648010). .. DNA and RNA extraction was primarily conducted at NYGC using the following protocol: PBMC normal samples were extracted with the QIAamp DNA Blood Mini Kit (Qiagen, 51106). .. Frozen tissue samples were extracted with Qiagen’s AllPrep kit (80204) if both DNA and RNA were needed, the QIAamp DNA Mini Kit (Qiagen, 51306) if only DNA was needed, or the RNeasy Mini kit (Qiagen, 74106) if only RNA was needed.

    Blocking Assay:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    Western Blot:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    DC Protein Assay:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    TUNEL Assay:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    Real-time Polymerase Chain Reaction:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..

    Derivative Assay:

    Article Title: AAV-based gene therapy with modified HEXB confers lasting therapeutic benefits in GM2 gangliosidosis models.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GM2-specific mouse mAb (GMB28: IgM) Provided by Dr. Tadashi Tai Kotani et al.65 Anti-NAG(A), antiserum against human HexA Provided by Dr. Akihiko Tsuji Izumi et al.,66 Utsumi et al.67 Anti-GFP antibody (JL-8) Takara Bio Cat# 632381; RRID: AB_2313808 anti-NeuN antibody Merck Millipore Cat# ABN78; RRID: AB_10807945 anti-GAPDH antibody (6C5) Santa Cruz Biotechnology Cat# ab8245; RRID: AB_2107448 anti-HexB antibody Abcam Cat# ab140649; RRID: AB_3065101 Anti-CD68 antibody (FA-11) Thermo Fisher Scientific Cat# 14-0681-82; RRID: AB_2572857 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7076; RRID: AB_330924 Goat Anti-Mouse IgG+IgM H&L (FITC) preadsorbed Abcam Cat# ab47830; RRID: AB_955209 Chemicals, peptides, and recombinant proteins 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide Sigma-Aldrich Cat# 474502 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide Merck Cat# 454428 Blocking One Nacalai Tesque Cat# 03953-95 Western Lightning Plus-ECL Perkin Elmer Cat# NEL104001EA Western Lightning Ultra Perkin Elmer Cat# NEL111001EA Hoechst33258 Sigma-Aldrich Cat# 94403 Tissue-Tek O.C.T. compound Sakura Finetek Japan Cat# 4583 HistoVT One Nacalai Tesque Cat# 06380-76 antigen retrieval buffer Abcam Cat# ab93684 AS-BI N-acetyl-β-D-glucosaminide Merck Cat# N4006 Critical commercial assays DC Protein Assay Bio-Rad Cat# 5000112 DeadEnd Colorimetric TUNEL system Promega Cat# G7360 VECTASTAIN Elite ABC Kit Vector Laboratories Cat# PK-6100 Tissue-Tek Mayer Hematoxylin For Prisma standard solution reservoir Sakura Finetek Japan Cat# 6186-4P Mouse CCL3/MIP-1 alpha ELISA Kit - Quantikine R&D Systems Cat# MMA00 QIAamp DNA Blood Mini Kit QIAGEN Cat# 51106 QIAamp 96 DNA QIAcube HT Kit QIAGEN Cat# 51331 QIAamp Fast DNA Stool Mini Kit QIAGEN Cat# 51604 TaqPath qPCR Master Mix Thermo Fisher Scientific Cat# A15297 Experimental models: Cell lines SH-SY5Y ATCC Cat# 94030304 RRID: CVCL_0019 iPSCs derived from patients with TSD Tanaka et al. (19) N/A (Continued on next page) Cell Reports Medicine 7, 102762, May 19, 2026 e1 Article ll OPEN ACCESS ..



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