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giemsa stain  (Thermo Fisher)


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    Thermo Fisher giemsa stain
    Giemsa Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/giemsa-diff-quick/pm39010826-71-10-14?v=Thermo+Fisher
    Average 96 stars, based on 1 article reviews
    giemsa stain - by Bioz Stars, 2026-08
    96/100 stars

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    giemsa staining kit diff-quick  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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    Nanjing Jiancheng Bioengineering Research Institute Co Ltd giemsa staining kit diff-quick
    Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) <t>Giemsa</t> <t>staining</t> was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.
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    Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

    Journal: International Journal of Oncology

    Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

    doi: 10.3892/ijo.2022.5368

    Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . (A) Giemsa staining was used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the activation and fusion of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate activated macrophages and red arrows indicate multinucleated giant cells. (B) Immunofluorescence staining were used to examine F4/80 and CD163 expression to identify the characteristics of activated macrophages and multinucleated giant cells. White arrows indicate activated macrophages and red arrow indicate multinucleated giant cells. Quantification of (C and D) the percentage of activated macrophages and (E and F) the percentage of multinucleated giant cells in the different groups. Data are presented as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01, *** P<0.001 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

    Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

    Techniques: Activation Assay, In Vitro, Staining, Immunofluorescence, Expressing

    Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

    Journal: International Journal of Oncology

    Article Title: Crosstalk between cancer and different cancer stroma subtypes promotes the infiltration of tumor-associated macrophages into the tumor microenvironment of oral squamous cell carcinoma

    doi: 10.3892/ijo.2022.5368

    Figure Lengend Snippet: Effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . (A) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages. Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (B) Transwell (migration) and Giemsa staining were used to examine the effects of VSCC-SCs, SCC-SCs and HDFs on the migration of macrophages following crosstalk with HSC-3 cells in vitro . Black arrows indicate spindle-shaped macrophages and red arrows indicate round-shaped macrophages. (C and D) Quantification of cell migration number in the different groups. Data are shown as the mean ± SD of one independent experiment and the independent experiments were repeated in triplicate. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05 and **** P<0.0001. ns, not significant (P>0.05); VSCC-SCs, verrucous squamous cell carcinoma-associated stromal cells; SCC-SCs, squamous cell carcinoma-associated stromal cells; HDFs, human dermal fibroblasts.

    Article Snippet: Giemsa staining was conducted using a Giemsa staining kit (Diff-Quick, Nanjing Jiancheng Bioengineering Institute).

    Techniques: Migration, In Vitro, Staining