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Microptic SL diff-quick staining
Diff Quick Staining, supplied by Microptic SL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa+staining+diff+quik+fix/diff+quick++staining/pmc07697408-77-19-21
Average 90 stars, based on 1 article reviews
diff-quick staining - by Bioz Stars, 2026-10
90/100 stars

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Diff-Quik:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Staining:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Concentration Assay:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.

Light Microscopy:

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria (Kruger et al. 1986, Kruger et al. 1988) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Outcomes of Intracytoplasmic Sperm Injection Cycles for Complete Teratozoospermia: A Case-Control Study Using Paired Sibling Oocytes
Article Snippet: In the current study, the Diff-Quick stain (Microptic S.L., Barcelona, Spain) was also used to confirm morphology results.

Article Title: Cumulus matrix selection leads to isolation of spermatozoa with better motility, morphology, and lower DNA fragmentation
Article Snippet: Sperm morphology in each sample was evaluated according to Kruger’s strict criteria ( Kruger et al. 1986 , Kruger et al. 1988 ) using the Diff-Quick staining Kit (Microptic SL, Barcelona, Spain).

Article Title: Effects of Ureaplasma urealyticum infection on semen quality and sperm morphology
Article Snippet: Semen smears were dyed using a Diff-Quick reagent (MICROPTIC S.L.

Article Title: Effect of season on the dynamics of cat sperm DNA fragmentation.
Article Snippet: Sperm morphology was examined with Diff-Quick® (Microptic S.L., Barcelona, Spain) staining.

Article Title: Colloid centrifugation reduces bacterial load in chilled dog semen.
Article Snippet: Conventional semen extenders contain antibiotics to prevent bacterial growth.. Finding alternatives would be beneficial to minimize the development of bacterial resistance mechanisms.. The aim of this study was to determine the effect of Single Layer Centrifugation (SLC) with Canicoll of dog semen on microbial load and sperm quality during cooled storage.

Article Title: Correlation of Prostatic Artery Blood Flow Assessed by Doppler Ultrasonography with Semen Characteristics in Beagle Dogs
Article Snippet: Sperm morphology was examined via light-microscopic evaluation (Olympus ® BH12, Olympus Optical Co., Barcelona, Spain) of smears stained with Diff-Quick ® (MICROPTIC S.L., Barcelona, Spain) staining.

Article Title: Bartonella infections in three species of Microtus : prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Article Snippet: Smears were air-dried, fixed in absolute methanol and stained with Diff Quick (Microptic SL, Barcelona, Spain) and Hemacolor (Merck, Darmstadt, Germany) staining kits, according to each manufacturer’s instructions.



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Medion Diagnostics giemsa staining diff quik fix
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Giemsa Staining Diff Quik Fix, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/giemsa+staining+diff+quik+fix/giemsa+staining+diff+quik+fix/pmc08217666-60-17-23
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Dade Behring wright-giemsa staining of fixed blood cells diff-quik
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
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Image Search Results


Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Journal: Frontiers in Immunology

Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

doi: 10.3389/fimmu.2021.636954

Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy