o ctober 2025 coding sequence (Addgene inc)

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Addgene inc o ctober 2025 coding sequence
O Ctober 2025 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+coding+sequence/Ub-G76V-GFP+(Plasmid+%2311941)/pm40795920-59-21-26
Average 92 stars, based on 65 article reviews

o ctober 2025 coding sequence - by Bioz Stars, 2026-10

92/100 stars

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Sequencing:

Article Title: Substrate structure determines p97- and RAD23A/B-mediated proteasomal degradation in human cells
Article Snippet: .. The Ub-G76V-GFP coding sequence (Addgene #11941) or Ub-G76V-GFP fused with a 65-amino-acid sequence derived from cyclin B containing an ssrA tag (sequence: AANDENYALAAHGGKHTFNNENVSARLGGACSIAVQAPAQHTFNNENVSARLGGALSIAVQAPAQ) ( ) was inserted into the pAID6.3N. ..

Article Title: Substrate Structure Determines p97- and RAD23A/B-Mediated Proteasomal Degradation in Human Cells.
Article Snippet: .. The Ub-G76V-GFP D ow nloaded from https://academ ic.oup.com /jb/advance-article/doi/10.1093/jb/m vaf046/8230199 by U niversidade Federal da Paraiba (U FPB) user on 14 O ctober 2025 coding sequence (Addgene #11941) or Ub-G76V-GFP fused with a 65-amino-acid sequence derived from cyclin B containing an ssrA tag (sequence: AANDENYALAAHGGKHTFNNENVSARLGGACSIAVQAPAQHTFNNENVSARLGGALSIAVQAPAQ) (42) was inserted into the pAID6.3N. ..

Derivative Assay:

Transfection:

Article Title: Carfilzomib-specific proteasome β5/β2 inhibition drives cardiotoxicity via remodeling of protein homeostasis and the renin-angiotensin-system
Article Snippet: .. H9c2 cardiomyoblasts were transfected by the calcium-phosphate method with the Ub G76V -GFP vector (Addgene #11941, USA, a kind gift from Nico Dantuma), encoding a fluorogenic proteasome substrate that accumulates proportionally to the degree of functional proteasome inhibition, as specified before. .. Cells with stable Ub G76V -GFP expression were selected with G418 (500 ng/ml, Gibco/Thermo Fisher Scientific Waltham, MA, USA) for 5 days.

Functional Assay:

Inhibition:

Plasmid Preparation:

Article Title: Predictable Modulation of a Spontaneous Post‐translational Modification in Living Cells
Article Snippet: A pDEST17 (Invitrogen) plasmid containing the dialAGEWT Ub sequence was used as a template for site-directed mutagenesis. .. All dialAGE variants were prepared using a QuikChange XL II kit (Agilent) with the following 5’-3’ primers: dialAGER42(+) Q49Y forward TCCGGCCATCTTCCAGATACTTTCCGGCGAAGATC dialAGER42(+) Q49Y reverse GATCTTCGCCGGAAAGTATCTGGAAGATGGCCGGA dialAGER42(-) Q49E forward GCCATCTTCCAGCTCCTTTCCGGCGAAGA dialAGER42(-) Q49E reverse TCTTCGCCGGAAAGGAGCTGGAAGATGGC dialAGER54(-) T55E forward GATGTTGTAGTCGCTCAGTTCCCGGCCATCTTCCA GCTG dialAGER54(-) T55E reverse CAGCTGGAAGATGGCCGGGAACTGAGCGACTACA ACATC dialAGER54(+) T55Y forward GTTGTAGTCGCTCAGGTACCGGCCATCTTCCAGC dialAGER54(+) T55Y reverse GCTGGAAGATGGCCGGTACCTGAGCGACTACAAC dialAGEQ49D Q49D forward CATCTTCCAGGTCCTTTCCGGCGAAGATCAG dialAGEQ49D Q49D reverse CTGATCTTCGCCGGAAAGGACCTGGAAGATG dialAGEQ49S Q49S forward CATCTTCCAGACTCTTTCCGGCGAAGATCAG dialAGEQ49S Q49S reverse CTGATCTTCGCCGGAAAGAGTCTGGAAGATG dialAGET55F T55F forward GTTGTAGTCGCTCAGGAACCGGCCATCTTCCAGC dialAGET55F T55F reverse GCTGGAAGATGGCCGGTTCCTGAGCGACTACAAC Ub-GFP G76V plasmid was obtained from Nico Dantuma (Addgene #11941). ..

Review