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GenScript corporation codon optimized cdna of full-length spike
Codon Optimized Cdna Of Full Length Spike, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genscript+cpass+kit/cpass+sars+cov+2+neutralization+antibody+detection+kit/pmc07872346-42-5-7
Average 90 stars, based on 1 article reviews
codon optimized cdna of full-length spike - by Bioz Stars, 2026-09
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Article Title: Trajectory of humoral responses to two doses of the ChAdOx1 nCoV-19 vaccination in patients receiving maintenance hemodialysis
Article Snippet: A US-FDA-authorized sVNT assay (cPass SARS-CoV-2 Neutralization Antibody Detection Kit, GenScript, Singapore) was used to quantify neutralizing antibodies against ancestral virus, delta and omicron (BA.1) at V2M1 and V2M5.

Article Title: Targeting heptad repeats and fusion peptide: nanoparticle vaccine elicits mucosal immune response against SARS-CoV-2 variants.
Article Snippet: Isolated cells were stimulated with a pool of SARS-CoV-2 spike (subunit 1 + subunit 2) peptide pools (GenScript, RP30223CN) for 1 h, in the presence of anti-CD28 (Biolegend, 302934) at a concentration of 1 μg/mL.

Article Title: Evaluating the distribution and clustering of SARS-CoV-2 antibodies in dogs across the United States of America.
Article Snippet: Serum samples collected from household dogs in this study were tested using blocking enzyme-linked immunosorbent assay (bELISA)31, Serum neutralization (SN)7, LumitTM Dx SARS-CoV-2 Immunoassay (Promega, Madison, Wisconsin), and cPassTM SARS-CoV-2 Neutralization Antibody Detection Assay (GenScript, Piscataway, New Jersey).

Article Title: Evaluating the distribution and clustering of SARS-CoV-2 antibodies in dogs across the United States of America
Article Snippet: Serum samples collected from household dogs in this study were tested using blocking enzyme-linked immunosorbent assay (bELISA) , Serum neutralization (SN) , LumitTM Dx SARS-CoV-2 Immunoassay (Promega, Madison, Wisconsin), and cPassTM SARS-CoV-2 Neutralization Antibody Detection Assay (GenScript, Piscataway, New Jersey).

Article Title: Quantitative characterisation of extracellular vesicles designed to decoy or compete with SARS-CoV-2 reveals differential mode of action across variants of concern and highlights the diversity of Omicron.
Article Snippet: As shown in Supplementary Table 1c, two different assays were applied for anti-SARS-CoV-2 screening according to manufacturer’s instructions: the Elecsys Anti-SARS-CoV-2 total anti-N antibody ECLIA (Roche Diagnostics) and a SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) (GenScript, Piscataway Town-ship, New Jersey, USA) using an ETIMax3000 platform (Diasorin, Saluggia, Italy).

Article Title: Comparative analysis of humoral immunity kinetics following three COVID-19 vaccines in a multi-ethnic cohort of medical students and healthcare professionals across Malaysia.
Article Snippet: A02161 was utilized as the positive control when the SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit (GenScript, Cat.No.

Inhibition:

Article Title: Compositions including Sbi adjuvants and methods of use thereof
Article Snippet: .. ACE2:RBD inhibition assay: The ability of vaccinated sera to inhibit ACE2:RBD binding was measured essentially as described in Nie, et al. (Nat Biotech 15: 3699-3715, 2020), using the SARS-CoV-2 Surrogate Virus Neutralization Test kit (GenScript). ..

Binding Assay:

Article Title: Compositions including Sbi adjuvants and methods of use thereof
Article Snippet: .. ACE2:RBD inhibition assay: The ability of vaccinated sera to inhibit ACE2:RBD binding was measured essentially as described in Nie, et al. (Nat Biotech 15: 3699-3715, 2020), using the SARS-CoV-2 Surrogate Virus Neutralization Test kit (GenScript). ..

Virus:

Article Title: Compositions including Sbi adjuvants and methods of use thereof
Article Snippet: .. ACE2:RBD inhibition assay: The ability of vaccinated sera to inhibit ACE2:RBD binding was measured essentially as described in Nie, et al. (Nat Biotech 15: 3699-3715, 2020), using the SARS-CoV-2 Surrogate Virus Neutralization Test kit (GenScript). ..

Neutralization:

Article Title: Compositions including Sbi adjuvants and methods of use thereof
Article Snippet: .. ACE2:RBD inhibition assay: The ability of vaccinated sera to inhibit ACE2:RBD binding was measured essentially as described in Nie, et al. (Nat Biotech 15: 3699-3715, 2020), using the SARS-CoV-2 Surrogate Virus Neutralization Test kit (GenScript). ..

Sequencing:

Article Title: HTRF-based identification of small molecules targeting SARS-CoV-2 E protein interaction with ZO-1 PDZ2
Article Snippet: .. The cDNA sequence encoding amino acids 45-75 of the SARS-CoV-2 E protein was synthesized and then cloned into the pET-15b vector by GenScript. ..

Synthesized:

Article Title: HTRF-based identification of small molecules targeting SARS-CoV-2 E protein interaction with ZO-1 PDZ2
Article Snippet: .. The cDNA sequence encoding amino acids 45-75 of the SARS-CoV-2 E protein was synthesized and then cloned into the pET-15b vector by GenScript. ..

Clone Assay:

Article Title: HTRF-based identification of small molecules targeting SARS-CoV-2 E protein interaction with ZO-1 PDZ2
Article Snippet: .. The cDNA sequence encoding amino acids 45-75 of the SARS-CoV-2 E protein was synthesized and then cloned into the pET-15b vector by GenScript. ..



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Comparison of ID50 values determined by the <t>sVNT</t> <t>ELISA</t> kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.
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Comparison of ID50 values determined by the <t>sVNT</t> <t>ELISA</t> kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.
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Comparison of ID50 values determined by the sVNT ELISA kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.

Journal: Frontiers in Immunology

Article Title: A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity

doi: 10.3389/fimmu.2022.1041860

Figure Lengend Snippet: Comparison of ID50 values determined by the sVNT ELISA kit and iACE2/RBD-Fc model. ID50 values of 7 vaccine serum samples and 4 convalescent plasma samples were determined by the commercial ELISA based sVNT kit and the iACE2/RBD-Fc model. A panel of raw data for determination of neutralization activity of one vaccine serum by the sVNT ELISA kit and the iACE2/RBD-Fc model was shown in (A, B) , respectively. Two sample dilution response inhibition curves generated from the data shown in Panel (A, B) were plotted and shown in (C) . ID50 values of 11 samples determined by the two assays were compared and shown in (D) . The dash line was used to indicated the serum dilution fold with an activity to inhibit 50% binding of the RBD-Fc on the ACE2 bead. p value <0.05 is represented as *.

Article Snippet: The ID50 (50% of maximal inhibitory dose) of samples were determined using an ELISA based sVNT kit (GenScript cPass SARS-CoV-2 neutralization Antibody detection kit, GenScript, China) according to instruction of the manufacturer with a minor modification.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Neutralization, Activity Assay, Inhibition, Generated, Binding Assay