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Jackson Laboratory genotyping protocol
Genotyping Protocol, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genotyping+protocols/genotyping+protocol/pm40328357-143-6-26
Average 90 stars, based on 1 article reviews
genotyping protocol - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Immunoprecipitation:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Western Blot:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Transfection:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

shRNA:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Fluorescence:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Transduction:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Concentration Assay:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Membrane:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Expressing:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Comparison:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

MANN-WHITNEY:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Cell Culture:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Knockdown:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Purification:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Virus:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Plasmid Preparation:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Recombinant:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

In Situ:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.

Software:

Article Title: Pharmacological regeneration of sensory hair cells restores afferent innervation and vestibular function
Article Snippet: Littermates were genotyped using protocols from The Jackson Laboratory ( ) and mated with Pou4f3 DTR/+ mice to obtain Pou4f3 DTR/+ ;Plp1 CreER ; mTmG mice.

Article Title: Liver TET1 promotes metabolic dysfunction-associated steatotic liver disease.
Article Snippet: The genotyping protocols for these mice were as the Jackson laboratory described, except TET1 KO.

Article Title: Phase-dependent iron depletion differentially regulates the niche of intestinal stem cells in experimental colitis via ERK/STAT3 signaling pathway
Article Snippet: The genotyping procedures were performed following the protocols provided by Jackson Laboratory.

Article Title: Oxytocinergic input from the paraventricular nucleus to the nucleus accumbens core modulates methamphetamine-conditioned place preference
Article Snippet: All OXT-Cre mice (6–8 weeks) were genotyped according to the protocols provided by Jackson Laboratory.

Article Title: Implications of endoplasmic reticulum stress and beta-cell loss in immunodeficient diabetic NRG-Akita mice for understanding monogenic diabetes
Article Snippet: Genotyping was performed according to Jackson Laboratory protocol (The Jackson Laboratory).

Article Title: Asialofetuin-Coupled Lipid-Based nanosystems to target the Asialoglycoprotein receptor: Delivering genes to hepatocytes for the treatment of Fabry disease.
Article Snippet: Exploiting the protein production capacity of hepatocytes for de novo expression of αGalactosidase A (α-Gal A) by gene supplementation therapy represents one of the most promising strategies for the treatment of Fabry disease (FD).. The asialoglycoprotein receptor (ASGPr) has proven to be one of the target receptors of choice for hepatocyte-directed nanomedicines, and natural glycoproteins such as asialofetuin (AF) can be used as specific ligands.. Herein, we have developed AF-decorated solid lipid nanoparticles (SLNs), prepared by different techniques and cationic lipid compositions, for restoring the enzyme deficiency in FD by gene supplementation targeted to hepatocytes.

Article Title: SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons
Article Snippet: Genotyping protocol provided by the Jackson Laboratory was used for the maintenance of the SNAP25 (+/−) mice.



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Image Search Results


Cre recombinase-expression and fluorescence signal in a Cd2-CreERT2 -inducible tdTomato mouse model. (A) Schematic diagram depicting the Cd2-CreERT2 mouse crossbred with a tdTomato reporter mouse line. (B) Schematic diagram depicting the Tamoxifen-induced Cre-recombinase. (C) Representative images of thymus, spleen and mesenteric lymph node (mLN) from Cd2-CreERT2;Rosa26-LSL-tdTomato mice visualized directly for tdTomato fluorescence. (D) Representative immunofluorescence images of tissue sections for tdTomato expression in CD2 + cells (green).

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: Cre recombinase-expression and fluorescence signal in a Cd2-CreERT2 -inducible tdTomato mouse model. (A) Schematic diagram depicting the Cd2-CreERT2 mouse crossbred with a tdTomato reporter mouse line. (B) Schematic diagram depicting the Tamoxifen-induced Cre-recombinase. (C) Representative images of thymus, spleen and mesenteric lymph node (mLN) from Cd2-CreERT2;Rosa26-LSL-tdTomato mice visualized directly for tdTomato fluorescence. (D) Representative immunofluorescence images of tissue sections for tdTomato expression in CD2 + cells (green).

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques: Expressing, Fluorescence, Immunofluorescence

tdTomato + /CD2 + cells analysis from flow cytometry measurements. Representative flow cytometry plots of tdTomato + /CD2 + cells from CD45 + subpopulations of peripheral blood (PB), thymus, spleen (SP) and mesenteric lymph node (mLN) in Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment. (C) Quantification of tdTomato + /CD2 + % cells, n=4.

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: tdTomato + /CD2 + cells analysis from flow cytometry measurements. Representative flow cytometry plots of tdTomato + /CD2 + cells from CD45 + subpopulations of peripheral blood (PB), thymus, spleen (SP) and mesenteric lymph node (mLN) in Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment. (C) Quantification of tdTomato + /CD2 + % cells, n=4.

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques: Flow Cytometry

CD2-Cre activity in mature leukocytes (T cells). Co-expression of CD2 and tdTomato in T cells from peripheral blood (PB), thymus, spleen (SP) and mesenteric lymph node (mLN) gated on CD3 + cells from Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment (A, B) and graph bar (C) . (D–F) gated on CD4 + cells from Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment (D, E) and graph bar (F) , (G–I) Gated on CD8 + cells from Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice, with or without tamoxifen treatment (G, H) and graph bar (I) . (J) Co-expression of CD2 and tdTomato in CD4 + CD8 + double-positive (DP) T cells from the thymus of Cd2-CreERT2;Rosa26-LSL-tdTomato female or male mice with or without tamoxifen treatment. n=4.

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: CD2-Cre activity in mature leukocytes (T cells). Co-expression of CD2 and tdTomato in T cells from peripheral blood (PB), thymus, spleen (SP) and mesenteric lymph node (mLN) gated on CD3 + cells from Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment (A, B) and graph bar (C) . (D–F) gated on CD4 + cells from Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) or male (B) mice with or without tamoxifen treatment (D, E) and graph bar (F) , (G–I) Gated on CD8 + cells from Cd2-CreERT2;Rosa26-LSL-tdTomato female (A) or male (B) mice, with or without tamoxifen treatment (G, H) and graph bar (I) . (J) Co-expression of CD2 and tdTomato in CD4 + CD8 + double-positive (DP) T cells from the thymus of Cd2-CreERT2;Rosa26-LSL-tdTomato female or male mice with or without tamoxifen treatment. n=4.

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques: Activity Assay, Expressing

tdTomato signal in B leukocyte populations. Numbers depict the percent of CD2 + tdTomato + cells in Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) and male mice (B) in peripheral blood (PB), Spleen (SP) and mesenteric lymph node (mLN) gated on CD19 + and the graph bar (C) . (D–H) Gated on CD19 + IgD - (D, E) , CD19 + IgD + (F, G) and graph bar (H) n=4.

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: tdTomato signal in B leukocyte populations. Numbers depict the percent of CD2 + tdTomato + cells in Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) and male mice (B) in peripheral blood (PB), Spleen (SP) and mesenteric lymph node (mLN) gated on CD19 + and the graph bar (C) . (D–H) Gated on CD19 + IgD - (D, E) , CD19 + IgD + (F, G) and graph bar (H) n=4.

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques:

tdTomato signal in NK leukocyte populations. Numbers depict the percent of CD2 + tdTomato + cells in Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) and male mice (B) in peripheral blood (PB), Spleen (SP) and mesenteric lymph node (mLN) gated on NK1.1 + CD3 - and the graph bar (C) n=4.

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: tdTomato signal in NK leukocyte populations. Numbers depict the percent of CD2 + tdTomato + cells in Cd2-CreERT2; Rosa26-LSL-tdTomato female (A) and male mice (B) in peripheral blood (PB), Spleen (SP) and mesenteric lymph node (mLN) gated on NK1.1 + CD3 - and the graph bar (C) n=4.

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques:

tdTomato expression in the indicated myeloid populations from Cd2-CreERT2;Rosa26-LSL-tdTomato mice. Flow cytometric analysis of tdTomato expression in macrophages (A) , granulocytes (B) monocytes (C) and dendritic cells (D) lineages. (E) The proportion of tdTomato + /CD2 + was charted as mean ± SEM, n = 4.

Journal: Frontiers in Immunology

Article Title: Generation and characterization of a tamoxifen-inducible lineage tracing tool Cd2-P2A-CreERT2 knock-in mice

doi: 10.3389/fimmu.2025.1482070

Figure Lengend Snippet: tdTomato expression in the indicated myeloid populations from Cd2-CreERT2;Rosa26-LSL-tdTomato mice. Flow cytometric analysis of tdTomato expression in macrophages (A) , granulocytes (B) monocytes (C) and dendritic cells (D) lineages. (E) The proportion of tdTomato + /CD2 + was charted as mean ± SEM, n = 4.

Article Snippet: The Cd2-CreERT2 mice were crossed with a loxP -flanked STOP cassette (LSL) Rosa26-LSL-tdTomato reporter line (AI9, Jackson Labs) ( ) to yield a Cd2 -driven tdTomato reporter mice ( Cd2-CreERT2;Rosa26-LSL-tdTomato ).The genotype of Cd2-CreERT2;Rosa26-LSL-tdTomato mice was identified using the same strategy as genotyping of Cd2-CreERT2 F2 generation for the Cd2 locus, and the protocol given by Jackson Labs for the Rosa26 locus.

Techniques: Expressing

Kaplan-Meier survival curve comparing ( A ) male 129 vEDS Map2k6 +/+ ( n = 17), vEDS Map2k6 +/– ( n = 20), and vEDS Map2k6 –/– mice ( n = 10) and ( B ) female 129 vEDS Map2k6 +/+ ( n = 19), vEDS Map2k6 +/– ( n = 15), and vEDS Map2k6 –/– mice ( n = 12). Significant differences were calculated using log-rank (Mantel-Cox) analysis (* P < 0.05; ** P < 0.01). ( C ) Immunoblot analysis of phosphorylated PKC at residue Ser 660 (pPKC) and phosphorylated ERK (pERK1/2) comparing aortic lysates obtained from the proximal descending aortas of mice at 2 months of age. ( D ) Quantification of p-PKC and p-ERK normalized to β-actin of control Map2k6 +/+ ( n = 3), vEDS Map2k6 +/+ ( n = 4), control Map2k6 –/– ( n = 7), and vEDS Map2k6 –/– ( n = 5) mice. P value refers to 2-way ANOVA with Holm-Šídák post hoc test (* P < 0.05, *** P < 0.001). ( E ) Immunofluorescence of sections from the proximal descending thoracic aorta of vEDS Map2k6 +/+ and vEDS Map2k6 –/– mice. The dashed line marks the approximate boundaries of the aortic wall. Scale bar is 50 microns. ( F ) Mean and total protein phosphatase 1 (PP1) dephosphorylation activity in aortic protein lysates from 129 vEDS Map2k6 +/+ mice ( n = 6) and 129 vEDS Map2k6 –/– ( n = 7). P value refers to unpaired t test with Welch’s correction (* P < 0.05, ** P < 0.01).DiFMU, 6,8-difluoro-7-hydroxy-4-methylcoumarin. In D and F , each symbol represents an independent biological replicate, with unfilled symbols representing male samples. Error bars show mean ± SEM. ( G ) Kaplan-Meier survival curve comparing control 129 vEDS Map2k6 –/– mice ( n = 33, 17 females and 16 males) with 129 vEDS Map2k6 –/– ( n = 11, 8 females and 3 males) mice receiving ruboxistaurin (PKC inhibitor) starting at postnatal day 21. Significant differences were calculated using log-rank (Mantel-Cox) analysis (* P < 0.05).

Journal: JCI Insight

Article Title: Map2k6 is a potent genetic modifier of arterial rupture in vascular Ehlers-Danlos syndrome mice

doi: 10.1172/jci.insight.187315

Figure Lengend Snippet: Kaplan-Meier survival curve comparing ( A ) male 129 vEDS Map2k6 +/+ ( n = 17), vEDS Map2k6 +/– ( n = 20), and vEDS Map2k6 –/– mice ( n = 10) and ( B ) female 129 vEDS Map2k6 +/+ ( n = 19), vEDS Map2k6 +/– ( n = 15), and vEDS Map2k6 –/– mice ( n = 12). Significant differences were calculated using log-rank (Mantel-Cox) analysis (* P < 0.05; ** P < 0.01). ( C ) Immunoblot analysis of phosphorylated PKC at residue Ser 660 (pPKC) and phosphorylated ERK (pERK1/2) comparing aortic lysates obtained from the proximal descending aortas of mice at 2 months of age. ( D ) Quantification of p-PKC and p-ERK normalized to β-actin of control Map2k6 +/+ ( n = 3), vEDS Map2k6 +/+ ( n = 4), control Map2k6 –/– ( n = 7), and vEDS Map2k6 –/– ( n = 5) mice. P value refers to 2-way ANOVA with Holm-Šídák post hoc test (* P < 0.05, *** P < 0.001). ( E ) Immunofluorescence of sections from the proximal descending thoracic aorta of vEDS Map2k6 +/+ and vEDS Map2k6 –/– mice. The dashed line marks the approximate boundaries of the aortic wall. Scale bar is 50 microns. ( F ) Mean and total protein phosphatase 1 (PP1) dephosphorylation activity in aortic protein lysates from 129 vEDS Map2k6 +/+ mice ( n = 6) and 129 vEDS Map2k6 –/– ( n = 7). P value refers to unpaired t test with Welch’s correction (* P < 0.05, ** P < 0.01).DiFMU, 6,8-difluoro-7-hydroxy-4-methylcoumarin. In D and F , each symbol represents an independent biological replicate, with unfilled symbols representing male samples. Error bars show mean ± SEM. ( G ) Kaplan-Meier survival curve comparing control 129 vEDS Map2k6 –/– mice ( n = 33, 17 females and 16 males) with 129 vEDS Map2k6 –/– ( n = 11, 8 females and 3 males) mice receiving ruboxistaurin (PKC inhibitor) starting at postnatal day 21. Significant differences were calculated using log-rank (Mantel-Cox) analysis (* P < 0.05).

Article Snippet: Map2k6 –/– mice were genotyped according to The Jackson Laboratory protocols.

Techniques: Western Blot, Residue, Control, Immunofluorescence, De-Phosphorylation Assay, Activity Assay