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10X Genomics barcoding
Mouse spermatogenesis single-cell RNA-seq datasets.
Barcoding, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genomics/barcoding+feature/pmc07302054-8-88-89
Average 86 stars, based on 1 article reviews
barcoding - by Bioz Stars, 2026-11
86/100 stars

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1) Product Images from "What has single-cell RNA-seq taught us about mammalian spermatogenesis?"

Article Title: What has single-cell RNA-seq taught us about mammalian spermatogenesis?

Journal: Biology of Reproduction

doi: 10.1093/biolre/ioz088

Mouse spermatogenesis single-cell RNA-seq datasets.
Figure Legend Snippet: Mouse spermatogenesis single-cell RNA-seq datasets.

Techniques Used: Biomarker Discovery, Knock-Out, RNAscope, Transplantation Assay, Sequencing

Human spermatogenesis single-cell RNA-seq datasets.
Figure Legend Snippet: Human spermatogenesis single-cell RNA-seq datasets.

Techniques Used: Biomarker Discovery, Sequencing

Related Articles

Sequencing:

Article Title: Comparative Analysis of Single-Nucleus and Single-Cell RNA Sequencing in Human Bone Marrow Mononuclear Cells: Methodological Insights and Trade-offs
Article Snippet: .. This feature enables the application of methods like Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), which utilises 10x Genomics’ Feature Barcoding technology. ..

Article Title: Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation.
Article Snippet: .. CROP-seq screening: Single-cell sequencing Excess supernatant was reduced using the VR NxT instrument (Menarini Silicon Biosystems) and processed using the Chromium Next GEM Single Cell 5’ v2 Dual Indexing Kit with Feature Barcoding (10x Genomics cat. No. 1000263), with the following modifications. ..

Single Cell:

Article Title: BACH2 shapes tissue resident memory T cell programs to promote HIV-1 persistence
Article Snippet: Raw fastq sequences from Chromium Single Cell Multiome ATAC + Gene Expression sequencing (ATAC & RNA) were demultiplexed with CellRanger-Arc v2 (10x Genomics) mkfastq and sequences were aligned to the hg38 reference genome using CellRanger-Arc count. .. Raw fastq sequences from Single Cell Gene Expression with Feature Barcoding (RNA + ADT/HTO) were demultiplexed with CellRanger v5 (10x Genomics) mkfastq and reads were aligned to the hg38 reference genome using CellRanger count. ..

Article Title: Tissue-adapted NK cells shape pathogenic cDC1 niches in early arthritis
Article Snippet: .. Innate lymphoid cell libraries were generated using the Chromium Next GEM Single Cell 5’ v2 kit with feature barcoding (10x Genomics, CG000330). .. DC and total immune cell libraries were generated using the Chromium Next GEM Single Cell 3’ v3.1 kit (10x Genomics, CG000204), modified for ADT and HTO recovery.

Article Title: Neutrophils drive vascular occlusion, tumour necrosis and metastasis
Article Snippet: Tumours were digested as previously stated, sorted for viability and stained with TotalSeq-B Mouse Universal Cocktail, V1.0 (199902, BioLegend), TotalSeq-B0109 anti-mouse CD16/32 antibody (101345, BioLegend) and TotalSeq-B0301-5 anti-mouse Hashtag 3 (BioLegend), all according to the manufacturer’s instructions. .. Samples were then used as input into the Chromium 3′ single-cell kit for feature barcoding (PN-1000121, 10X Genomics). ..

Article Title: Human LFA-1 governs T cell immune surveillance of the skin
Article Snippet: Cells were washed three times with PBS supplemented with 0.2% BSA and subjected to single-cell capture on the 10X Genomics Chromium platform. .. Libraries were generated with the Chromium Next GEM Automated Single-Cell 5′ Kit v2 with Feature Barcoding (10X Genomics) according to the manufacturer’s protocol. .. The purified libraries were sequenced on the Illumina NovaSeq 6000 System (S4 flow cell, 150-bp paired-end reads), with a median depth of 50,000 reads per cell for gene expression.

Article Title: Integrated time-series analysis and high-content CRISPR screening delineate the dynamics of macrophage immune regulation.
Article Snippet: .. CROP-seq screening: Single-cell sequencing Excess supernatant was reduced using the VR NxT instrument (Menarini Silicon Biosystems) and processed using the Chromium Next GEM Single Cell 5’ v2 Dual Indexing Kit with Feature Barcoding (10x Genomics cat. No. 1000263), with the following modifications. ..

Article Title: Spontaneous network activity links misrouted interneuron migration to cortical maturation abnormalities
Article Snippet: A papain dissociation system (Worthington, LK003150) was used as described ( ) on the gentleMACS Octo Dissociator (Miltenyi Biotec) to generate a cell suspension. tdT + cells were sorted on BD FACSAria III (BD FACSDiva Software, v.8.0.2) with a 100 μm nozzle and subjected to downstream processing on the 10x Genomics Chromium platform. .. After sorting in PBS (Lonza, 17-516) with 0.02% BSA (B9000, NEB), ∼12’000 individual cells per sample were loaded onto a 10X Genomics Chromium platform for gel beads-in-emulsion and complementary DNA generation, carrying cell- and transcript-specific barcodes using the Chromium Single Cell 3’ Reagent Kit v.3.1 with Feature Barcoding technology (10X Genomics, PN-1000121) following the manufacturer’s protocol (document no. CG000205, 10X Genomics). .. 10x Genomics libraries were sequenced on an Illumina NovaSeq at the Genomics Core Facility of the Helmholtz Center Munich.

Gene Expression:

Article Title: BACH2 shapes tissue resident memory T cell programs to promote HIV-1 persistence
Article Snippet: Raw fastq sequences from Chromium Single Cell Multiome ATAC + Gene Expression sequencing (ATAC & RNA) were demultiplexed with CellRanger-Arc v2 (10x Genomics) mkfastq and sequences were aligned to the hg38 reference genome using CellRanger-Arc count. .. Raw fastq sequences from Single Cell Gene Expression with Feature Barcoding (RNA + ADT/HTO) were demultiplexed with CellRanger v5 (10x Genomics) mkfastq and reads were aligned to the hg38 reference genome using CellRanger count. ..

Generated:

Article Title: Tissue-adapted NK cells shape pathogenic cDC1 niches in early arthritis
Article Snippet: .. Innate lymphoid cell libraries were generated using the Chromium Next GEM Single Cell 5’ v2 kit with feature barcoding (10x Genomics, CG000330). .. DC and total immune cell libraries were generated using the Chromium Next GEM Single Cell 3’ v3.1 kit (10x Genomics, CG000204), modified for ADT and HTO recovery.

Article Title: Human LFA-1 governs T cell immune surveillance of the skin
Article Snippet: Cells were washed three times with PBS supplemented with 0.2% BSA and subjected to single-cell capture on the 10X Genomics Chromium platform. .. Libraries were generated with the Chromium Next GEM Automated Single-Cell 5′ Kit v2 with Feature Barcoding (10X Genomics) according to the manufacturer’s protocol. .. The purified libraries were sequenced on the Illumina NovaSeq 6000 System (S4 flow cell, 150-bp paired-end reads), with a median depth of 50,000 reads per cell for gene expression.



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