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pkd1flox flox ksp cre shibazaki  (Addgene inc)


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    Addgene inc pkd1flox flox ksp cre shibazaki
    Pkd1flox Flox Ksp Cre Shibazaki, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genomics+suite+(software+analysis+microarray+data/pEF+1-PSN-4+(Plasmid+%2320084)/pm38039961-533-113-107
    Average 92 stars, based on 1 article reviews
    pkd1flox flox ksp cre shibazaki - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Single Cell:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A

    Gene Expression:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A

    RNA Sequencing:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A

    Microarray:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A

    Polymerase Chain Reaction:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A

    Software:

    Article Title: Human iPSC-derived renal collecting duct organoid model cystogenesis in ADPKD.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER iMatrix-511 silk Nippi 892021 IWR-1 MERCK I0161-5MG KnockOut Serum Replacement Thermo Fisher Scientific 10828028 LDN193189 Axon Medchem Axon1509 L-methionine sulfone FUJIFILM Wako A17027 Mannitol Nacalai Tesque 11662–42 Methanol Nacalai Tesque 21914–03 Normal donkey serum MERCK 566460 Puromycin Nacalai Tesque 14861–71 Rapamycin FUJIFILM Wako R0161 R-spondin 1 R&D 4645-RS-250 STEM-CELLBANKER GMP grade ZENOAQ RESOURCE ZR646 Stem Fit AK02N Ajinomoto AK02N Sucrose Nacalai Tesque 30403–55 Sunflower oil Sigma Aldrich 8001-21-6 Tesevatinib MedChemExpress HY-13314 Thiazovivin Santa Cruz SCB-SC-361380-10 Tolvaptan Axon medchem Axon1591 Trimesate FUJIFILM Wako 26–2340 Triton X-100 Nacalai Tesque 35501–15 TTNPB Santa Cruz sc-203303 Venglustat Selleck biotech E0646 Y-27632 FUJIFILM Wako 034–24024 Critical commercial assays Chromium Next GEM Single Cell 30 Reagent Kit v3.1 10x Genomics PN-1000268 Ex Taq TaKaRa RR001B FuGENE6 Transfection Reagent Promega E2691 Gateway LR Clonase II Enzyme mix Thermo Fisher Scientific 11791020 HMT 5 kDa ultrafiltration tube Human Metabolome Technologies UFC3LCCNB-HMT Human 8 3 60K LncRNA expression array ArrayStar N/A In Fusion HD Cloning Kit TaKaRa 639648 KOD Fx Neo TOYOBO KFX-201 Lipofectamine Stem Transfection Reagent Thermo Fisher Scientific STEM00001 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs E7490 NucleoSpin RNA XS TaKaRa 740902.5 pENTRTM Directional TOPO Cloning Kits Thermo Fisher Scientific K240020SP ReverTra Ace TOYOBO TRT-101 RNeasy Mini kit Qiagen 74106 SMART-Seq Stranded Kit TaKaRa 634442 TB Green Premix Ex Taq II TaKaRa RR820A UN-L kit serotec A666-00 Wizard SV Gel and PCR Clean-UP System Promega A9281 Deposited data RNA sequencing data of UBTCs cultured with or without A83-01 This paper E-GEAD-558 RNA sequencing data of expanded UBTCs This paper E-GEAD-559 Single cell RNA sequencing data of CD organoids This paper GSE237528 (Continued on next page) Cell Reports 42, 113431, December 26, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Single cell gene expression profile of hPSC-derived CD organoids (day 18) Shi et al., 202212 GSE206153 RNA sequencing data of PKD1+/+ UBTCs and PKD1 / UBTCs This paper GSE215875 Microarray data of cyst cells treated with or without TTNPB This paper E-GEAD-560 Experimental models: Cell lines Human: 585A1 iPSC line RIKEN BRC HPS0354 Human: 1231A3 iPSC line RIKEN BRC HPS0381 Human: 1383D2 iPSC line RIKEN BRC HPS1005 Human: 1383D6 iPSC line RIKEN BRC HPS1006 Experimental models: Plasmids pHL-EF1a-hcPBase-A Ishida et al., 201819 N/A pPV-H1-gRNA-mEF1a-RiH Ishida et al., 201819 Addgene: 100598 pPV-TetO-SphcCas9-iC-A-EF1a-rtTA-iP Ishida et al., 201819 Addgene: 100596 PB-TAG-ERN Kim et al., 201655 Addgene: 80476 Experimental models: Organisms/strains Mouse: Pkd1flox/flox: Ksp-Cre Shibazaki et al., 200845 N/A Oligonucleotides Primers for PCR, see Table S6 This paper N/A Software and algorithms BZ-X Analyzer KEYENCE BZ-X700, BZ-X800 CellRanger v7.0.1 10x Genomics https://support.10xgenomics.com/ EZR Kanda. .. 201356 http://www.jichi.ac.jp/saitama-sct/SaitamaHP. files/statmedEN.html GeneSpring GX v12.1 Agilent N/A MasterHands Sugimoto et al., 201057 N/A Monocle v2.22.0 Qiu et al., 201758 http://cole-trapnell-lab.github.io/monocle-release/ Python 3 Python Software Foundation N/A Prism 7 GraphPad Software https://www.graphpad.com/scientific-software/ prism/ RStudio RStudio Team, 2020 N/A Seurat package v4.1.1 Stuart et al., 201959 http://satijalab.org/seurat/ WinROOF Mitani Corporation N/A



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