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next-generation sequencing data analysis workflow  (Thermo Fisher)


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    Structured Review

    Thermo Fisher next-generation sequencing data analysis workflow
    Next Generation Sequencing Data Analysis Workflow, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generation+sequencing+data+analysis/Thermo+Sequenase+Cycle+Sequencing+Kit/pm37224176-79-15-20
    Average 90 stars, based on 1 article reviews
    next-generation sequencing data analysis workflow - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: First Evidence of Rickettsia conorii Infection in Dogs in Northern Tunisia
    Article Snippet: The positive PCR products were purified using the ExoSAP cleanup procedure (Amersham Biosciences, Piscataway, NJ, USA). .. All nucleotide sequences were obtained using the Big Dye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) and the 3130 automated sequencer (Applied Biosystems, CA, USA). .. The sequences generated in this study were edited and aligned using BioEdit v7.7.1.0 https://bioedit.software.informer.com/7.1/ (accessed on 20 May 2023) [28] and ClustalW software programs (CLUSTAL 2.0.12 Multiple Sequence Alignments) http://www.clustal. org/clustal2/ (accessed on 20 May 2023) [29].

    Article Title: Latent infection of an active giant endogenous virus in a unicellular green alga
    Article Snippet: The two representative isolates from both lakes (Ors24 and Kgh138) were selected for purification and sequencing of the mcp PCR products using the MinElute PCR Purification Kit (Qiagen, Valencia, CA, USA), following the manufacturer’s instructions. .. Sequencing was performed in an Applied Biosystems 8-capillary 3500 Genetic Analyzer using the BigDye Terminator Cycle Sequencing Kit (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Multi-Year Comparison of VITEK® MS performance for identification of rarely encountered pathogenic gram-negative bacilli (GNBs) in a large integrated Canadian healthcare region
    Article Snippet: .. 16S rRNA gene sequencing was performed by fast PCR/cycle sequencing using fast MicroSEQTM 500 16S DNA PCR kits and an ABI Prism 3500 XL sequencer (Applied Biosystems, ThermoFisher Scientific, Foster City, CA) as previously described( ). .. SmartGene’s Integrated Database Network System (IDNS3®) (Lausanne, Switzerland) bacterial database was used to determine closely related species ( https://www.Smartgene.com ).

    Article Title: Molecular phylogeny of the Lecudinoidea (Apicomplexa): A major group of marine gregarines with diverse shapes, movements and hosts.
    Article Snippet: .. PCR products were purified using ExoSap (Applied Biosystems™), and then the purified PCR products were sequenced by Sanger sequencing by Sequencing and Bioinformatics Consortium at the University of British Columbia, Canada, using the same primers used for PCR amplification. ..

    Polymerase Chain Reaction:

    Article Title: Multi-Year Comparison of VITEK® MS performance for identification of rarely encountered pathogenic gram-negative bacilli (GNBs) in a large integrated Canadian healthcare region
    Article Snippet: .. 16S rRNA gene sequencing was performed by fast PCR/cycle sequencing using fast MicroSEQTM 500 16S DNA PCR kits and an ABI Prism 3500 XL sequencer (Applied Biosystems, ThermoFisher Scientific, Foster City, CA) as previously described( ). .. SmartGene’s Integrated Database Network System (IDNS3®) (Lausanne, Switzerland) bacterial database was used to determine closely related species ( https://www.Smartgene.com ).

    Article Title: Molecular phylogeny of the Lecudinoidea (Apicomplexa): A major group of marine gregarines with diverse shapes, movements and hosts.
    Article Snippet: .. PCR products were purified using ExoSap (Applied Biosystems™), and then the purified PCR products were sequenced by Sanger sequencing by Sequencing and Bioinformatics Consortium at the University of British Columbia, Canada, using the same primers used for PCR amplification. ..

    Article Title: Arid1a Loss Enhances Disease Progression in a Murine Model of Osteosarcoma
    Article Snippet: .. Splinkerette PCR analysis with Ion Torrent DNA sequencing was used following a published protocol for the detection of transposon insertion sites in the mouse genome [ ]. ..

    other:

    Article Title: Widespread exposure to SARS-CoV-2 in wildlife communities.
    Article Snippet: To amplify the entire SARS-CoV-2 genome of the 2022 Virginia opossum, positive samples were reverse transcribed and amplified using SuperScript IV Reverse Transcriptase (Thermofisher) and ARTIC nCoV-2019 Amplicon Panel V4.1 primers (Integrated DNA technologies).

    Article Title: Long noncoding RNA EPCART regulates translation through PI3K/AKT/mTOR pathway and PDCD4 in prostate cancer.
    Article Snippet: The Sanger sequencing was performed using BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) and 3500xL Genetic Analyzer (Applied Biosystems) according to the manufacturer’s instructions.

    Purification:

    Article Title: Molecular phylogeny of the Lecudinoidea (Apicomplexa): A major group of marine gregarines with diverse shapes, movements and hosts.
    Article Snippet: .. PCR products were purified using ExoSap (Applied Biosystems™), and then the purified PCR products were sequenced by Sanger sequencing by Sequencing and Bioinformatics Consortium at the University of British Columbia, Canada, using the same primers used for PCR amplification. ..

    Amplification:

    Article Title: Molecular phylogeny of the Lecudinoidea (Apicomplexa): A major group of marine gregarines with diverse shapes, movements and hosts.
    Article Snippet: .. PCR products were purified using ExoSap (Applied Biosystems™), and then the purified PCR products were sequenced by Sanger sequencing by Sequencing and Bioinformatics Consortium at the University of British Columbia, Canada, using the same primers used for PCR amplification. ..

    DNA Sequencing:

    Article Title: Arid1a Loss Enhances Disease Progression in a Murine Model of Osteosarcoma
    Article Snippet: .. Splinkerette PCR analysis with Ion Torrent DNA sequencing was used following a published protocol for the detection of transposon insertion sites in the mouse genome [ ]. ..



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