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Corning Life Sciences microarray slide
Microarray Slide, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/genepix+pro+6%2E0+microarray+image+analysis+software/microarray+slides/us09587256-1275-7-9
Average 90 stars, based on 1 article reviews
microarray slide - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Purification:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Before scanning at 532 nm for Cy3 and 633 nm for Cy5 (Agilent Technologies, Foster City, CA), slides were successively washed at room temperature in 0.5× SSC/0.1% SDS for 2 min, 0.5× SSC for 2 min (twice), and 0.06× SSC for 2 min.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Reverse Transcription:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Transcriptional response analysis Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Labeling:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Transcriptional response analysis Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42°C. .. Slides were scanned on a GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA), and raw intensities were quantitated using GenePix pro version 6.0.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Microarray:

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). ..

Article Title: A mutant (‘lab strain’) of the hyperthermophilicarchaeon Pyrococcusfuriosus , lacking flagella, has unusual growthphysiology
Article Snippet: RNA was purified using an RNEasy kit (Qiagen, Valencia, CA). .. RNA was then reverse transcribed to cDNA using Superscript III (Invitrogen), random primers (Invitrogen), and the incorporation ofaminoallyl-DUTP, 5-(3-aminoallyl)-2’-deoxyuridine 5’-triphosphate (Ambion, Austin, TX), as described previously ( Chou et al. 2007 ). cDNA was labeled with either Cy3 or Cy5 dye (GE Healthcare, Little Chalfont, United Kingdom), and hybridized to one of six microarray slides (Corning, Acton, MA) in the loop design (see Figure S1 ), following protocols previously described( Chou et al. 2007 ). .. Transcriptional response analysis Slides were scanned using an Axon scanner (Sunnyvale, CA) and quantified using GenePix Pro, version 6.0 software.

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Before scanning at 532 nm for Cy3 and 633 nm for Cy5 (Agilent Technologies, Foster City, CA), slides were successively washed at room temperature in 0.5× SSC/0.1% SDS for 2 min, 0.5× SSC for 2 min (twice), and 0.06× SSC for 2 min.

Article Title: Conversion of 4-Hydroxybutyrate to Acetyl Coenzyme A and Its Anapleurosis in the Metallosphaera sedula 3-Hydroxypropionate/4-Hydroxybutyrate Carbon Fixation Pathway
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse transcribed with Superscript III (Invitrogen), repurified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42°C. .. Slides were scanned on a GenePix 4000B microarray scanner (Molecular Devices, Sunnyvale, CA), and raw intensities were quantitated using GenePix pro version 6.0.

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: .. Total RNA was extracted and purified (RNeasy; Qiagen), reverse transcribed (Superscript III; Invitrogen), re-purified, labeled with either Cy3 or Cy5 dye (GE Healthcare), and hybridized to the microarray slides (Corning). .. Formation of the CoA ester was also confirmed using HPLC (Waters).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Article Title: Sequestration of carbon dioxide with hydrogen to useful products
Article Snippet: Total RNA was extracted and purified using an RNeasy kit (Qiagen), reverse-transcribed with Superscript III (Invitrogen), re-purified, and labeled with either Cy3 or Cy5 dye (GE Healthcare). .. Labeled cDNA was then hybridized to the microarray slide (Corning) at 42° C. Slides were scanned on a GenePix 4000B Microarray Scanner (Molecular Devices, Sunnyvale, Calif.) and raw intensities were quantitated using GenePix Pro version 6.0. .. Data normalization and statistical analysis were performed using JMP Genomics 5 (SAS, Cary, N.C.).

Saline:

Article Title: A cAMP-Dependent, Protein Kinase A-Independent Signaling Pathway Mediating Neuritogenesis through Egr1 in PC12 Cells
Article Snippet: Probes were then labeled with N -hydroxy-succinimide ester dye Cy3 or Cy5 (Amersham). .. After denaturation, purified Cy3/Cy5-labeled cDNA samples were combined and hybridized on a microarray slide in a humidified chamber (Corning Life Sciences) at 65°C overnight in the presence of 5× saline-sodium citrate (SSC), 0.1% SDS, 25% formamide and polyA (25 ng/ μ l). .. Before scanning at 532 nm for Cy3 and 633 nm for Cy5 (Agilent Technologies, Foster City, CA), slides were successively washed at room temperature in 0.5× SSC/0.1% SDS for 2 min, 0.5× SSC for 2 min (twice), and 0.06× SSC for 2 min.



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