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peroxidase igg fraction monoclonal mouse anti rabbit igg  (Jackson Immuno)


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    Jackson Immuno peroxidase igg fraction monoclonal mouse anti rabbit igg

    Peroxidase Igg Fraction Monoclonal Mouse Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 532 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+specific+analysis+gsa+algorithm/Peroxidase+IgG+Fraction+Monoclonal+Mouse+Anti-Rabbit+IgG%2C+light+chain+specific/pmc11703504-9-2-13
    Average 96 stars, based on 532 article reviews
    peroxidase igg fraction monoclonal mouse anti rabbit igg - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy"

    Article Title: Plural molecular and cellular mechanisms of pore domain KCNQ2 encephalopathy

    Journal: eLife

    doi: 10.7554/eLife.91204


    Figure Legend Snippet:

    Techniques Used: Transfection, Construct, Mutagenesis, Recombinant, Expressing, Plasmid Preparation, Gene Expression, Real-time Polymerase Chain Reaction, Sequencing, Western Blot, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Incubation, Software

    Related Articles

    Western Blot:

    Article Title: Gait analysis for functional evaluation in a surgical hindlimb suspension model of muscle atrophy
    Article Snippet: WGA‐488 , IF , Lectin conjugate , IgG , 1:500 , Invitrogen , W11261. .. Peroxidase IgG fraction monoclonal mouse anti‐rabbit IgG, light chain specific , WB , Rabbit (monoclonal) , IgG , 1:3000 , Jackson ImmunoResearch , 211‐032‐171. .. AffiniPure goat anti‐mouse IgG, light chain specific , WB , Mouse (monoclonal) , IgG , 1:3000 , Jackson ImmunoResearch , 115‐005‐174.

    Article Title: Chromatin stability safeguards mitochondrial homeostasis and restrains TORC1 signaling.
    Article Snippet: .. Membranes then were incubated with HRP-conjugated secondary antibody (mouse anti-rabbit peroxidase-conjugated IgG (cat# 211-032-171) or goat anti-mouse peroxidase-conjugated IgG (cat# 115-035-174) from Jackson Immuno Research), washed extensively, and then IBs were developed using the Immobilon Western Chemiluminescent HRP Substrate system. .. IBs were analyzed with ImageJ, and the band intensity data were used to generate graphical data using GraphPad Prism.

    Article Title: Gait analysis for functional evaluation in a surgical hindlimb suspension model of muscle atrophy
    Article Snippet: .. Target/marker Application Target species/type Ab isotype Dilution Company Catalogue no. PAX7 IF Mouse (monoclonal) mIgG1 1:10 DSHB PAX7 MyHC (type I) IF Mouse (monoclonal) mIgG2b 1:50 DSHB BA-F8 MyHC (type IIA) IF Mouse (monoclonal) mIgG1 1:500 DSHB SC-71 MyHC (type IIB) IF Mouse (monoclonal) mIgM 1:50 DSHB BF-F3 MG29 (Sypl2) IF/WB Mouse (monoclonal, custom) IgG IF: 1:100/WB: 1:1000 Custom-made – MG53 (Trim72) IF/WB Rabbit (monoclonal, custom) IgG IF: 1:200/WB: 1:3000 Custom-made – GAPDH WB Mouse (monoclonal) IgG 1:5000 ABclonal AC002 WGA-488 IF Lectin conjugate IgG 1:500 Invitrogen W11261 Peroxidase IgG fraction monoclonal mouse anti-rabbit IgG, light chain specific WB Rabbit (monoclonal) IgG 1:3000 Jackson ImmunoResearch 211-032-171 AffiniPure goat anti-mouse IgG, light chain specific WB Mouse (monoclonal) IgG 1:3000 Jackson ImmunoResearch 115-005-174 Goat anti-mouse IgG2b cross-adsorbed secondary antibody, Alexa Fluor 488 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21141 Goat anti-mouse IgG1 cross-adsorbed secondary antibody, Alexa Fluor 568 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21124 Goat anti-mouse IgM (heavy chain) secondary antibody, Alexa Fluor 647 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21238 Note: Primary and secondary antibodies used in this study are listed with their target, host species, application, isotype, working dilution, source and catalogue number. ..

    Incubation:

    Article Title: Chromatin stability safeguards mitochondrial homeostasis and restrains TORC1 signaling.
    Article Snippet: .. Membranes then were incubated with HRP-conjugated secondary antibody (mouse anti-rabbit peroxidase-conjugated IgG (cat# 211-032-171) or goat anti-mouse peroxidase-conjugated IgG (cat# 115-035-174) from Jackson Immuno Research), washed extensively, and then IBs were developed using the Immobilon Western Chemiluminescent HRP Substrate system. .. IBs were analyzed with ImageJ, and the band intensity data were used to generate graphical data using GraphPad Prism.

    Article Title: Muscleblind-like proteins dimerize by forming disulfide bonds to regulate alternative splicing and pathogenic RNA foci formation
    Article Snippet: .. The following day, membranes were incubated with IRDye 680LT donkey anti-mouse (1:20000, LICOR, 926-68072) and either IRDye 680LT donkey anti-rabbit (1:20000, LICOR, 926-68073) or HRP mouse anti-rabbit IgG light chain (1:5000, Jackson ImmunoResearch, 211-032-171), diluted in 5% BSA in PBS-T, for 1 hour at RT. .. All blots were also incubated with Rhodamine-conjugated α-GAPDH human Fab fragment (1:1000, Bio-Rad, 12004168).

    other:

    Article Title: PTEN-L inhibits mitophagy and GSDME-dependent pyroptosis via Parkin dephosphorylation.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models HeLa cells ATCC CCL-2 HeLa stably expressing YFP-Parkin (HeLa YFPParkin) Dr. Richard J. Youle N/A HeLa YFP-Parkin/PTENL OE This study N/A HeLa YFP-Parkin/PTENL KO This study N/A HeLa YFP-Parkin/PTEN OE This study N/A HeLa YFP-Parkin/PTEN and PTEN-L DKO This study N/A HeLa YFP-Parkin/PTEN only This study N/A HeLa YFP-Parkin/ GSDME KO This study N/A HeLa YFP-Parkin/PTENL OE/GSDME KO This study N/A HeLa YFP-Parkin/ BAX and BAK DKD This study N/A HeLa YFP-Parkin/PTENL OE/BAX and BAK DKD This study N/A HeLa YFP-Parkin/MCL-1 KD This study N/A HeLa YFP-Parkin/PTENL OE/MCL-1 KD This study N/A HeLa YFP-Parkin/NOXA KD This study N/A HeLa YFP-Parkin/PTENL OE/NOXA KD This study N/A HeLa YFP-Parkin/mitokeima This study N/A HeLa YFP-Parkin/mitokeima/PTEN-L OE This study N/A HeLa stably expressing HA-Parkin (HeLa HAParkin) This study N/A SH-SY5Y cells ATCC CRL-2266 SH-SY5Y stably expressing YFP-Parkin (SH-SY5Y YFP-Parkin) This study N/A SH-SY5Y/PTEN-L OE This study N/A SH-SY5Y/ PTEN-L KO This study N/A SH-SY5Y/GSDME KD This study N/A Reagent/resource Reference or source Identifier or catalog number HEK293T cells ATCC CL-0005 Recombinant DNA pCMV-Tag2B-PTEN-L This study N/A pCMV-Tag2B-PTENLC297S This study N/A pCMV-Tag2B-PTENLG302R This study N/A pCMV-Tag2B-PTEN This study N/A pCMV-Tag2B-Parkin This study N/A pmcherry-PTEN-L This study N/A pET30a-WT PTEN-L This study N/A pET30a-PTEN-LC297S This study N/A pET30a-PTEN-LG302R This study N/A pCDH-HA-GSDME This study N/A pCDH-HA-GSDME D267A/ D270A This study N/A lentiCRISPR v2-sgPTEN-L This study N/A lentiCRISPR v2sgGSDME This study N/A lentiCRISPR v2-sgPTEN and PTEN-L This study N/A pPLKO.1-shBAX and BAK This study N/A pPLKO.1-shGSDME This study N/A pPLKO.1-shMCL-1 This study N/A pPLKO.1-shNOXA This study N/A pEBG vector Dr. Jongkyeong Chung N/A pEBG-Parkin WT Dr. Jongkyeong Chung N/A pEBG-Parkin S65D Dr. Jongkyeong Chung N/A eYFP-C1-Parkin Dr. Richard J. Youle N/A pCDH-YFP-Parkin-puro Dr. Han-Ming Shen N/A pEGFP-C1-MCL-1 This study N/A pRK5-HA-Ub Addgene 17608 pEGFP-N1-PINK1 Dr. Kah-Leong Lim N/A Antibodies Rabbit anti-Caspase-3 Cell Signalling Technology 9662 Rabbit anti-Cleaved Caspase-3 Cell Signalling Technology 9661 Rabbit anti-Caspase-9 Cell Signalling Technology 9502 Rabbit anti-PARP Cell Signalling Technology 9542 Rabbit anti-PINK1 Cell Signalling Technology 6946 Rabbit anti-GFP Cell Signalling Technology 2956 © The Author(s) EMBO reports 17 Reagent/resource Reference or source Identifier or catalog number Mouse anti-Parkin Cell Signalling Technology 4211 Rabbit anti-PTEN Cell Signalling Technology 138G6 Rabbit anti-PTEN Cell Signalling Technology 9559 Rabbit anti-BCl-2 Cell Signalling Technology 2876 Rabbit anti-BAK Cell Signalling Technology 12105 Rabbit anti-GSDMD Cell Signalling Technology 39754 Rabbit anti-GAPDH Cell Signalling Technology 2118 Mouse anti-Actin Sigma-Aldrich A5441 Mouse anti-Tubulin Sigma-Aldrich T6199 Rabbit anti-GSDMD Proteintech 20770-1-AP Mouse anti-Flag ABclonal AE005 Rabbit anti-LDHA ABclonal A0861 Mouse anti-HA ABclonal AE008 Rabbit anti-PMAIP1 (NOXA) ABclonal A9801 Mouse anti-α1 sodium potassium ATPase Abcam ab215191 Rabbit anti-pSer65Parkin Abcam ab7671 Rabbit anti-BFL-1 (BCL2A1) Abcam ab315376 Rabbit anti-GSDME Abcam ab287160 Mouse anti-Tim23 BD Biosciences 611223 Mouse anti-BAX BD Biosciences 610983 Mouse anti-Cytochrome c BD Biosciences 556433 Mouse anti-Cytochrome c Promab P20788 Mouse anti-BAX(6A7) Novus Biologicals NBP1-28566 Rabbit anti-MCL-1 Santa Cruz Biotechnology S-19 Mouse anti-MCL-1 Santa Cruz Biotechnology sc-819 Mouse anti-Tom20 Santa Cruz Biotechnology sc-17764 Mouse anti-Alexa Fluor 647 Cytochrome c BioLegend 612310 Mouse anti-IgG1 kappa Isotype Control Invitrogen 51-4714-81 Mouse anti-Alexa Fluor 647 Invitrogen A-21235 Rabbit anti-Alexa Fluor 647 Invitrogen A-21245 Mouse anti-Alexa Fluor 488 Invitrogen A-32723 Reagent/resource Reference or source Identifier or catalog number Rabbit anti-Alexa Fluor 488 Invitrogen A-32731 Mouse anti-Alexa Fluor 555 Invitrogen A-32727 Rabbit anti-Alexa Fluor 555 Invitrogen A-32732 Mouse anti-IgG, light chain specific Jackson ImmunoResearch 115-035-174 Rabbit anti-IgG, light chain specific Jackson ImmunoResearch 211-032-171 Mouse anti-IgG-HRP Absin abs20039 Rabbit anti-IgG-HRP Absin abs20040 Chemicals, enzymes and other reagents Q-VD-OPh MedChemExpress HY-12305 Staurosporine MedChemExpress HY-15141 TNF-α MedChemExpress HY-P7058 Cisplatin MedChemExpress HY-17394 Doxorubicin MedChemExpress HY-15142 Ferrostatin-1 MedChemExpress HY-100579 Necrostatin-1 MedChemExpress HY-15760 Necrostatin 2 racemate MedChemExpress HY-14622 Cycloheximide MedChemExpress HY-12320 ABT-737 MedChemExpress HY-50907 TAK-243 MedChemExpress HY-100487 Oligomycin Sigma-Aldrich 75351 Antimycin Sigma-Aldrich A8674 Carbonyl cyanide 3-chlorophenylhydrazone Sigma-Aldrich C2759 Z-VAD-FMK Targetmol T6013 S63845 Targetmol T5346 MG132 Targetmol T001-10011605 DMSO Solarbio D8371 Bafilomycin A1 Selleck S1413 Phosphatase inhibitor TargetMol C0004 Proteinase inhibitor cocktail TargetMol C0001 PVDF membrane Merck TPVH00010[334485] Enhanced chemiluminescence 4A BIOTECH 4AW011-200 Fetal Bovine Serum Excell FSP500 Opti-MEM Gibco 31985-070 Medium Dulbecco’s Modified Eagle Medium Procell PM150210 TAE buffer Biosharp BL533A TS-GelRed nucleic acid gel stain Ver.2 TSINGKE TSJ003 Puromycin Dihydrochloride Yeasen 60210ES25 Polybrene Beyotime C0351 18 EMBO reports © The Author(s) Reagent/resource Reference or source Identifier or catalog number GZ-C07 Lipo8000 Beyotime C0533 AgeI-HF NEW ENGLAND Biolabs R3552S EcoRI-HF NEW ENGLAND Biolabs R3101S BamHI-HF NEW ENGLAND Biolabs R3136S BsmBI-v2 NEW ENGLAND Biolabs R0739S Propidium iodide Sangon Biotech A601112 SYTOX Green staining Thermo Fisher S7020 Oligonucleotides and other sequence-based reagents si GSDME#1 Homo sapiens GCGGUCCUAUUU GAUGAUGAA si GSDME#2 Homo sapiens GAUGAUGGAGU AUCUGAUCUU si BAX#1 Homo sapiens GGGACGAACU GGACAGUAA si BAX#2 Homo sapiens AAGGUGCCGGA ACUGAUCAGA si GSDMD#1 Homo sapiens GUGUGUCAACC UGUCUAUCAA si GSDMD#2 Homo sapiens CAGCACCUCAA UGAAUGUGUA si BAK#1 Homo sapiens CCCAUUCAC UACAGGUGAA si BAK#2 Homo sapiens GUACGAAGA UUCUUCAAAU BAX KD Homo sapiens CCGGGACGAACTG GACAGTAACATGCTCGAG CATGTTACTGTCCAG TTCGTCTTTTTG BAK KD Homo sapiens CCGGTGGTACG AAGATTCTTCAAATC TCGAGATTTGAAGAA TCTTCGTACCATTTTTG GSDME KD Homo sapiens CCGGGATGATGGA GTATCTGATCTTC TCGAGAAGATCAG ATACTCCATCAT CTTTTTG MCL-1 KD Homo sapiens CCGGGCCTAGTTTATCA CCAATAATCTCGAG ATTATTGGTGATA AACTAGGCTTTTTG NOXA KD Homo sapiens CCGGGCATTGTTGTTGT TGCTGTTTCTCGAGAAACA GCAACAACAACAAT GCTTTTTG GSDME KO Homo sapiens CACCGTAAGTTAC AGCTTCTAAGTC PTEN-L KO Homo sapiens CACCGCGGCGGCAC ATCCAGGGACC PTEN/PTEN-L DKO Homo sapiens CACCGTCATCTGG ATTATAGACCAG PTEN-L only Homo sapiens CCAGACATAACA GCCATCATCAA Reagent/resource Reference or source Identifier or catalog number Real-time quantitative polymerase chain reaction oligonucleotides Homo GSDME TGATCTCTGA GCACATGCAG TCCTTGGTG ACATTCCCATC Homo MCL-1 CCAAGAAAGCT GCATCGAACCAT CAGCACATTCC TGATGCCACCT Homo BAX TGACGGCAAC TTCAACTGGG AGCACTCCC GCCACAAAGA Homo GAPDH GACCCCTTCA TTGACCTCAAC CTTCTCCATG GTGGTGAAGA Software GraphPad Prism https://www.

    Whole Genome Amplification:

    Article Title: Gait analysis for functional evaluation in a surgical hindlimb suspension model of muscle atrophy
    Article Snippet: .. Target/marker Application Target species/type Ab isotype Dilution Company Catalogue no. PAX7 IF Mouse (monoclonal) mIgG1 1:10 DSHB PAX7 MyHC (type I) IF Mouse (monoclonal) mIgG2b 1:50 DSHB BA-F8 MyHC (type IIA) IF Mouse (monoclonal) mIgG1 1:500 DSHB SC-71 MyHC (type IIB) IF Mouse (monoclonal) mIgM 1:50 DSHB BF-F3 MG29 (Sypl2) IF/WB Mouse (monoclonal, custom) IgG IF: 1:100/WB: 1:1000 Custom-made – MG53 (Trim72) IF/WB Rabbit (monoclonal, custom) IgG IF: 1:200/WB: 1:3000 Custom-made – GAPDH WB Mouse (monoclonal) IgG 1:5000 ABclonal AC002 WGA-488 IF Lectin conjugate IgG 1:500 Invitrogen W11261 Peroxidase IgG fraction monoclonal mouse anti-rabbit IgG, light chain specific WB Rabbit (monoclonal) IgG 1:3000 Jackson ImmunoResearch 211-032-171 AffiniPure goat anti-mouse IgG, light chain specific WB Mouse (monoclonal) IgG 1:3000 Jackson ImmunoResearch 115-005-174 Goat anti-mouse IgG2b cross-adsorbed secondary antibody, Alexa Fluor 488 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21141 Goat anti-mouse IgG1 cross-adsorbed secondary antibody, Alexa Fluor 568 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21124 Goat anti-mouse IgM (heavy chain) secondary antibody, Alexa Fluor 647 IF Mouse (polyclonal) IgG 1:500 Invitrogen A21238 Note: Primary and secondary antibodies used in this study are listed with their target, host species, application, isotype, working dilution, source and catalogue number. ..



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