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peroxidase igg fraction monoclonal mouse anti goat igg  (Jackson Immuno)


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    Structured Review

    Jackson Immuno peroxidase igg fraction monoclonal mouse anti goat igg

    Peroxidase Igg Fraction Monoclonal Mouse Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+specific+analysis+gsa+algorithm/Peroxidase+IgG+Fraction+Monoclonal+Mouse+Anti-Goat+IgG%2C+light+chain+specific/pmc08647022-75-0-11
    Average 93 stars, based on 59 article reviews
    peroxidase igg fraction monoclonal mouse anti goat igg - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Promyelocytic leukemia protein targets MK2 to promote cytotoxicity"

    Article Title: Promyelocytic leukemia protein targets MK2 to promote cytotoxicity

    Journal: EMBO Reports

    doi: 10.15252/embr.202052254


    Figure Legend Snippet:

    Techniques Used: Generated, Transgenic Assay, Recombinant, Plasmid Preparation, Blocking Assay, Transfection, Antibody Labeling, Software, Cell Viability Assay

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    Article Snippet: The following chemicals were used for this study: PIK-93 (MedChem, HY-12046), RO-3306 (VWR, 102,516-264), GSK8612 (Cayman Chemical, 28,813–5), bafilomycin A 1 (Fisher Scientific, NC0910155), and Halo TMR ligand (Promega, G8252).

    Western Blot:

    Article Title: Functional interaction between Vangl2 and N-cadherin regulates planar cell polarization of the developing neural tube and cochlear sensory epithelium
    Article Snippet: List of antibodies Antigen Host Manufacturer Clone ID or Product number Application (Dilution) Conjugation Activin RIIB Mouse R&D systems MAB3393 IF (1:200) b-catenin Mouse BD Biosciences 14/Beta-Catenin WB (1:1000) Digoxigenin Sheep (Fab fragment) Roche 11093274910 in situ (1:2000) AP c-Jun Rabbit Cell Signaling Technology 60A8 IF (1:200) Phospho-c-Jun (Phospho-Ser63) Rabbit Cell Signaling Technology E617P IF (1:200) Myo7A Rabbit Proteus Biosciences 25-6790 IF (1:500) Mypt1 Rabbit Cell Signaling Technology D6C1 WB (1:500) Phsopho-Mypt1 (Phospho-Thr696) Rabbit Cell Signaling Technology #5163 WB (1:500) Phospho-Mypt1 (Phospho-Thr853) Rabbit Cell Signaling Technology #4563 WB(1:1000) N-cadherin Mouse BD Biosciences Clone 32/N-Cadherin WB, IF (1:1000) Nestin Mouse Thermo scientific Rat401 (4D4) IF (1:200)WB (1:500) a-Tubulin Mouse Proteintech 66031-1-Ig WB (1:1000) Vangl2 Goat Santa Cruz N-13 IP, IF (1:100)WB (1:500) N.A. .. Goat Santa Cruz sc-3887 (normal Goat IgG) IP (1:200) Goat IgG Donkey Life Technologies A-32814 IF (1:500) Alexa 488 Goat IgG Donkey Santa Cruz sc-2020 WB (1:5000) HRP Goat IgG Light Chain Specific Mouse Jackson ImmunoResearch 205-032-176 WB (1:5000) HRP Mouse IgG Donkey Life Technologies A-10037 IF (1:500) Alexa 568 Mouse IgG Goat Life Technologies A-11004 IF (1:500) Alexa 568 Mouse IgG Goat Bio-Rad 170-6516 WB (1:5000) HRP Rabbit IgG Goat Life Technologies A-11008 IF (1:500) Alexa 488 Rabbit IgG Goat Santa Cruz sc-2030 WB (1:5000) HRP ..

    Article Title: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
    Article Snippet: Membranes were incubated overnight at 4 °C with antibodies reactive to the following proteins: IGFBP7 polyclonal antibody (Thermo Fisher Scientific, PA1-86872, 1:1,000); anti-IGFBP7 (EPR11913(B)) (Abcam, ab170932, 1:1,000); Rb mAb to IGFBP3 (Abcam, ab193910, 1:1,000); CTGF (Abcam, ab6992, 1:1,000); anti-53BP1 (Abcam, ab36823, 1:1,000); anti-p21 (EPR18021) (Abcam, ab188224, 1:1,000); anti-p16ARC (EP1551Y) (Abcam, ab51243, 1:1,000); anti-histone H3 (Abcam, ab1791, 1:1,000); phospho-p53 (Ser392) (Cell Signaling Technology, 9281, 1:1,000); acetyl-p53 (Lys379) (Cell Signaling Technology, 2570, 1:1,000); p53 (Developmental Studies Hybridoma Bank (DSHB) product PCRP-TP53-1F7, 0.5 μg ml−1; PCRP-TP53-1F7 was deposited to the DSHB by Protein Capture Reagents Program, produced by Johns Hopkins University/CDI Laboratories); phospho-IGF-1 receptor β (Thy1135) (Cell Signaling Technology, 3918, 1:500); IGF-1 receptor β (Cell Signaling Technology, 9750, 1:500); phospho-IRS-1 (Ser612) (Cell Signaling Technology, 3203, 1:500); phospho-IRS-1 (Ser318) (Cell Signaling Technology, 5610, 1:500); IRS-1 (Cell Signaling Technology, 3407, 1:500); anti-IRS1 (Millipore, 06-248, 1:1,000); IRS-2 antibody (Cell Signaling Technology, 4502, 1:1,000); INSR antibody (18-44) (Thermo Fisher Scientific, MA1-10865, 1:1,000); phospho-Akt (Ser473) (D9E) (Cell Signaling Technology, 4060, 1:1,000); phospho-Akt (Thr308) (Cell Signaling Technology, 4056, 1:1,000); Akt (pan) (C67E7) (Cell Signaling Technology, 4691, 1:1,000); phosphor-FOXO3A (S253) (EPR1951(2)) (Abcam, ab154786, 1:1,000); FOXO3A (Abcam, ab109629,1ː1,000); insulin receptor β (C18C4) (Enzo, ADI-905-683, 1:1,000); anti-cGAS (Cell Signaling Technology, 15102, 1:1,000); anti-STING (MilliporeSigma, MABF270, 1 μg ml−1); anti-DDB1 (Abcam, ab109027, 1:50,000); anti-SOD2 (acetyl K68) (Abcam, ab137037, 1:1,000); and anti-SOD2 (Abcam, ab68155, 1:1,000). .. Blots were incubated with HRP-conjugated goat anti-mouse IgG (Bio-Rad, 170-5047, 1:100,000); goat anti-rabbit IgG (Bio-Rad, 170-5046, 1:100,000); and monoclonal mouse anti-goat IgG ( Jackson ImmunoResearch, 205-032-176, 1:50,000) and developed using Clarity Western ECL Substrate (Bio-Rad, 170-5061) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34580). .. To detect low-abundant protein, SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) or SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate (Thermo Fisher Scientific, A38544) was used.

    Article Title: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
    Article Snippet: Membranes were incubated overnight at 4 °C with antibodies reactive to the following proteins: IGFBP7 polyclonal antibody (Thermo Fisher Scientific, PA1-86872, 1:1,000); anti-IGFBP7 (EPR11913(B)) (Abcam, ab170932, 1:1,000); Rb mAb to IGFBP3 (Abcam, ab193910, 1:1,000); CTGF (Abcam, ab6992, 1:1,000); anti-53BP1 (Abcam, ab36823, 1:1,000); anti-p21 (EPR18021) (Abcam, ab188224, 1:1,000); anti-p16ARC (EP1551Y) (Abcam, ab51243, 1:1,000); anti-histone H3 (Abcam, ab1791, 1:1,000); phospho-p53 (Ser392) (Cell Signaling Technology, 9281, 1:1,000); acetyl-p53 (Lys379) (Cell Signaling Technology, 2570, 1:1,000); p53 (Developmental Studies Hybridoma Bank (DSHB) product PCRP-TP53-1F7, 0.5 μg ml −1 ; PCRP-TP53-1F7 was deposited to the DSHB by Protein Capture Reagents Program, produced by Johns Hopkins University/CDI Laboratories); phospho-IGF-1 receptor β (Thy1135) (Cell Signaling Technology, 3918, 1:500); IGF-1 receptor β (Cell Signaling Technology, 9750, 1:500); phospho-IRS-1 (Ser612) (Cell Signaling Technology, 3203, 1:500); phospho-IRS-1 (Ser318) (Cell Signaling Technology, 5610, 1:500); IRS-1 (Cell Signaling Technology, 3407, 1:500); anti-IRS1 (Millipore, 06-248, 1:1,000); IRS-2 antibody (Cell Signaling Technology, 4502, 1:1,000); INSR antibody (18-44) (Thermo Fisher Scientific, MA1-10865, 1:1,000); phospho-Akt (Ser473) (D9E) (Cell Signaling Technology, 4060, 1:1,000); phospho-Akt (Thr308) (Cell Signaling Technology, 4056, 1:1,000); Akt (pan) (C67E7) (Cell Signaling Technology, 4691, 1:1,000); phosphor-FOXO3A (S253) (EPR1951(2)) (Abcam, ab154786, 1:1,000); FOXO3A (Abcam, ab109629,1ː1,000); insulin receptor β (C18C4) (Enzo, ADI-905-683, 1:1,000); anti-cGAS (Cell Signaling Technology, 15102, 1:1,000); anti-STING (MilliporeSigma, MABF270, 1 μg ml −1 ); anti-DDB1 (Abcam, ab109027, 1:50,000); anti-SOD2 (acetyl K68) (Abcam, ab137037, 1:1,000); and anti-SOD2 (Abcam, ab68155, 1:1,000). .. Blots were incubated with HRP-conjugated goat anti-mouse IgG (Bio-Rad, 170-5047, 1:100,000); goat anti-rabbit IgG (Bio-Rad, 170-5046, 1:100,000); and monoclonal mouse anti-goat IgG (Jackson ImmunoResearch, 205-032-176, 1:50,000) and developed using Clarity Western ECL Substrate (Bio-Rad, 170-5061) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34580). .. To detect low-abundant protein, SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) or SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate (Thermo Fisher Scientific, A38544) was used.

    Incubation:

    Article Title: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
    Article Snippet: Membranes were incubated overnight at 4 °C with antibodies reactive to the following proteins: IGFBP7 polyclonal antibody (Thermo Fisher Scientific, PA1-86872, 1:1,000); anti-IGFBP7 (EPR11913(B)) (Abcam, ab170932, 1:1,000); Rb mAb to IGFBP3 (Abcam, ab193910, 1:1,000); CTGF (Abcam, ab6992, 1:1,000); anti-53BP1 (Abcam, ab36823, 1:1,000); anti-p21 (EPR18021) (Abcam, ab188224, 1:1,000); anti-p16ARC (EP1551Y) (Abcam, ab51243, 1:1,000); anti-histone H3 (Abcam, ab1791, 1:1,000); phospho-p53 (Ser392) (Cell Signaling Technology, 9281, 1:1,000); acetyl-p53 (Lys379) (Cell Signaling Technology, 2570, 1:1,000); p53 (Developmental Studies Hybridoma Bank (DSHB) product PCRP-TP53-1F7, 0.5 μg ml−1; PCRP-TP53-1F7 was deposited to the DSHB by Protein Capture Reagents Program, produced by Johns Hopkins University/CDI Laboratories); phospho-IGF-1 receptor β (Thy1135) (Cell Signaling Technology, 3918, 1:500); IGF-1 receptor β (Cell Signaling Technology, 9750, 1:500); phospho-IRS-1 (Ser612) (Cell Signaling Technology, 3203, 1:500); phospho-IRS-1 (Ser318) (Cell Signaling Technology, 5610, 1:500); IRS-1 (Cell Signaling Technology, 3407, 1:500); anti-IRS1 (Millipore, 06-248, 1:1,000); IRS-2 antibody (Cell Signaling Technology, 4502, 1:1,000); INSR antibody (18-44) (Thermo Fisher Scientific, MA1-10865, 1:1,000); phospho-Akt (Ser473) (D9E) (Cell Signaling Technology, 4060, 1:1,000); phospho-Akt (Thr308) (Cell Signaling Technology, 4056, 1:1,000); Akt (pan) (C67E7) (Cell Signaling Technology, 4691, 1:1,000); phosphor-FOXO3A (S253) (EPR1951(2)) (Abcam, ab154786, 1:1,000); FOXO3A (Abcam, ab109629,1ː1,000); insulin receptor β (C18C4) (Enzo, ADI-905-683, 1:1,000); anti-cGAS (Cell Signaling Technology, 15102, 1:1,000); anti-STING (MilliporeSigma, MABF270, 1 μg ml−1); anti-DDB1 (Abcam, ab109027, 1:50,000); anti-SOD2 (acetyl K68) (Abcam, ab137037, 1:1,000); and anti-SOD2 (Abcam, ab68155, 1:1,000). .. Blots were incubated with HRP-conjugated goat anti-mouse IgG (Bio-Rad, 170-5047, 1:100,000); goat anti-rabbit IgG (Bio-Rad, 170-5046, 1:100,000); and monoclonal mouse anti-goat IgG ( Jackson ImmunoResearch, 205-032-176, 1:50,000) and developed using Clarity Western ECL Substrate (Bio-Rad, 170-5061) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34580). .. To detect low-abundant protein, SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) or SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate (Thermo Fisher Scientific, A38544) was used.

    Article Title: Insulin-like growth factor-binding protein-7 (IGFBP7) links senescence to heart failure
    Article Snippet: Membranes were incubated overnight at 4 °C with antibodies reactive to the following proteins: IGFBP7 polyclonal antibody (Thermo Fisher Scientific, PA1-86872, 1:1,000); anti-IGFBP7 (EPR11913(B)) (Abcam, ab170932, 1:1,000); Rb mAb to IGFBP3 (Abcam, ab193910, 1:1,000); CTGF (Abcam, ab6992, 1:1,000); anti-53BP1 (Abcam, ab36823, 1:1,000); anti-p21 (EPR18021) (Abcam, ab188224, 1:1,000); anti-p16ARC (EP1551Y) (Abcam, ab51243, 1:1,000); anti-histone H3 (Abcam, ab1791, 1:1,000); phospho-p53 (Ser392) (Cell Signaling Technology, 9281, 1:1,000); acetyl-p53 (Lys379) (Cell Signaling Technology, 2570, 1:1,000); p53 (Developmental Studies Hybridoma Bank (DSHB) product PCRP-TP53-1F7, 0.5 μg ml −1 ; PCRP-TP53-1F7 was deposited to the DSHB by Protein Capture Reagents Program, produced by Johns Hopkins University/CDI Laboratories); phospho-IGF-1 receptor β (Thy1135) (Cell Signaling Technology, 3918, 1:500); IGF-1 receptor β (Cell Signaling Technology, 9750, 1:500); phospho-IRS-1 (Ser612) (Cell Signaling Technology, 3203, 1:500); phospho-IRS-1 (Ser318) (Cell Signaling Technology, 5610, 1:500); IRS-1 (Cell Signaling Technology, 3407, 1:500); anti-IRS1 (Millipore, 06-248, 1:1,000); IRS-2 antibody (Cell Signaling Technology, 4502, 1:1,000); INSR antibody (18-44) (Thermo Fisher Scientific, MA1-10865, 1:1,000); phospho-Akt (Ser473) (D9E) (Cell Signaling Technology, 4060, 1:1,000); phospho-Akt (Thr308) (Cell Signaling Technology, 4056, 1:1,000); Akt (pan) (C67E7) (Cell Signaling Technology, 4691, 1:1,000); phosphor-FOXO3A (S253) (EPR1951(2)) (Abcam, ab154786, 1:1,000); FOXO3A (Abcam, ab109629,1ː1,000); insulin receptor β (C18C4) (Enzo, ADI-905-683, 1:1,000); anti-cGAS (Cell Signaling Technology, 15102, 1:1,000); anti-STING (MilliporeSigma, MABF270, 1 μg ml −1 ); anti-DDB1 (Abcam, ab109027, 1:50,000); anti-SOD2 (acetyl K68) (Abcam, ab137037, 1:1,000); and anti-SOD2 (Abcam, ab68155, 1:1,000). .. Blots were incubated with HRP-conjugated goat anti-mouse IgG (Bio-Rad, 170-5047, 1:100,000); goat anti-rabbit IgG (Bio-Rad, 170-5046, 1:100,000); and monoclonal mouse anti-goat IgG (Jackson ImmunoResearch, 205-032-176, 1:50,000) and developed using Clarity Western ECL Substrate (Bio-Rad, 170-5061) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, 34580). .. To detect low-abundant protein, SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) or SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate (Thermo Fisher Scientific, A38544) was used.

    SDS Page:

    Article Title: Biliverdin reductase B as a new target in breast cancer
    Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN cat#AF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).

    Immunodetection:

    Article Title: Biliverdin reductase B as a new target in breast cancer
    Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN cat#AF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).

    Marker:

    Article Title: Biliverdin reductase B as a new target in breast cancer
    Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN cat#AF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).



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